Expression of neural cell adhesion molecule in human liver development and in congenital and acquired liver diseases

2001 ◽  
Vol 116 (3) ◽  
pp. 233-239 ◽  
Author(s):  
Louis Libbrecht ◽  
David Cassiman ◽  
Valeer Desmet ◽  
Tania Roskams
Keyword(s):  
Cell Adhesion ◽  
Adhesion Molecule ◽  
Liver Diseases ◽  
Human Liver ◽  
Cell Adhesion Molecule ◽  
Neural Cell Adhesion Molecule ◽  
Neural Cell ◽  
Liver Development ◽  
Neural Cell Adhesion

Expression of neural cell adhesion molecule (N-CAM) in perisinusoidal stellate cells of the human liver

1995 ◽  
Vol 283 (1) ◽  
pp. 159-165 ◽  
Author(s):  
Kazuki Nakatani ◽  
Shuichi Seki ◽  
Norifumi Kawada ◽  
Kenzo Kobayashi ◽  
Kenji Kaneda
Keyword(s):  
Cell Adhesion ◽  
Adhesion Molecule ◽  
Human Liver ◽  
Cell Adhesion Molecule ◽  
Neural Cell Adhesion Molecule ◽  
Stellate Cells ◽  
Neural Cell ◽  
Neural Cell Adhesion

scholarly journals Regulation of Neural Cell Adhesion Molecule Polysialylation: Evidence for Nontranscriptional Control and Sensitivity to an Intracellular Pool of Calcium

1998 ◽  
Vol 140 (5) ◽  
pp. 1177-1186 ◽  
Author(s):  
Juan L. Brusés ◽  
Urs Rutishauser
Keyword(s):  
Cell Adhesion ◽  
Adhesion Molecule ◽  
Cell Adhesion Molecule ◽  
Neural Cell Adhesion Molecule ◽  
Calcium Ionophore ◽  
Cytosolic Calcium ◽  
Neural Cell ◽  
Ciliary Ganglion ◽  
Cellular Mechanisms ◽  
Neural Cell Adhesion

The up- and downregulation of polysialic acid–neural cell adhesion molecule (PSA–NCAM) expression on motorneurons during development is associated respectively with target innervation and synaptogenesis, and is regulated at the level of PSA enzymatic biosynthesis involving specific polysialyltransferase activity. The purpose of this study has been to describe the cellular mechanisms by which that regulation might occur. It has been found that developmental regulation of PSA synthesis by ciliary ganglion motorneurons is not reflected in the levels of polysialyltransferase-1 (PST) or sialyltransferase-X (STX) mRNA. On the other hand, PSA synthesis in both the ciliary ganglion and the developing tectum appears to be coupled to the concentration of calcium in intracellular compartments. This study documents a calcium dependence of polysialyltransferase activity in a cell-free assay over the range of 0.1–1 mM, and a rapid sensitivity of new PSA synthesis, as measured in a pulse–chase analysis of tissue explants, to calcium ionophore perturbation of intracellular calcium levels. Moreover, the relevant calcium pool appears to be within a specific intracellular compartment that is sensitive to thapsigargin and does not directly reflect the level of cytosolic calcium. Perturbation of other major second messenger systems, such as cAMP and protein kinase–dependent pathways, did not affect polysialylation in the pulse chase analysis. These results suggest that the shuttling of calcium to different pools within the cell can result in the rapid regulation of PSA synthesis in developing tissues.

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