Organic and Biological Analytes

2012 ◽  
pp. 62-93
Keyword(s):  
Biological Analytes

A Photoactivable Formaldehyde Donor with Fluorescence Monitoring Reveals Threshold to Arrest Cell Migration

2019 ◽  
Author(s):  
Lukas P Smaga ◽  
Nicholas W Pino ◽  
Gabriela E Ibarra ◽  
Vishnu Krishnamurthy ◽  
Jefferson Chan
Keyword(s):  
Wound Healing ◽  
Cell Migration ◽  
Fluorescence Enhancement ◽  
Biological Effects ◽  
Cellular Systems ◽  
Live Cells ◽  
First Report ◽  
Cell Lysate ◽  
Intracellular Release ◽  
Biological Analytes

Controlled light-mediated delivery of biological analytes enables the investigation of highly reactivity molecules within cellular systems. As many biological effects are concentration dependent, it is critical to determine the location, time, and quantity of analyte donation. In this work, we have developed the first photoactivatable donor for formaldehyde (FA). Our optimized photoactivatable donor, photoFAD-3, is equipped with a fluorescence readout that enables monitoring of FA release with a concomitant 139-fold fluorescence enhancement. Tuning of photostability and cellular retention enabled quantification of intracellular FA release through cell lysate calibration. Application of photoFAD-3 uncovered the concentration range necessary for arresting wound healing in live cells. This marks the first report where a photoactivatable donor for any analyte has been used to quantify intracellular release.

A Thread/Fabric-Based Band as A Flexible and Wearable Microfluidic Device for Sweat Sensing and Monitoring

Lab on a Chip ◽  
2021 ◽  
Author(s):  
Zhiqi Zhao ◽  
Qiujin Li ◽  
Linna Chen ◽  
Yu Zhao ◽  
Jixian Gong ◽  
...  
Keyword(s):  
Microfluidic Device ◽  
Point Of Care ◽  
Monitoring Systems ◽  
Biological Analytes

Flexible biosensors for monitoring systems have emerged as a promising portable diagnostics platform due to their potential for in situ point-of-care (POC) analytic devices. Assessment of biological analytes in sweat...

scholarly journals Carbon Nanomaterials as Versatile Platforms for Biosensing Applications

Micromachines ◽  
2020 ◽  
Vol 11 (9) ◽  
pp. 814
Author(s):  
Hye Suk Hwang ◽  
Jae Won Jeong ◽  
Yoong Ahm Kim ◽  
Mincheol Chang
Keyword(s):  
Metallic Nanoparticles ◽  
Carbon Nanomaterials ◽  
Measuring System ◽  
Mechanical And Electrical Properties ◽  
Target Analytes ◽  
Wide Range ◽  
Biological Recognition ◽  
Simple Molecules ◽  
Living Organisms ◽  
Biological Analytes

A biosensor is defined as a measuring system that includes a biological receptor unit with distinctive specificities toward target analytes. Such analytes include a wide range of biological origins such as DNAs of bacteria or viruses, or proteins generated from an immune system of infected or contaminated living organisms. They further include simple molecules such as glucose, ions, and vitamins. One of the major challenges in biosensor development is achieving efficient signal capture of biological recognition-transduction events. Carbon nanomaterials (CNs) are promising candidates to improve the sensitivity of biosensors while attaining low detection limits owing to their capability of immobilizing large quantities of bioreceptor units at a reduced volume, and they can also act as a transduction element. In addition, CNs can be adapted to functionalization and conjugation with organic compounds or metallic nanoparticles; the creation of surface functional groups offers new properties (e.g., physical, chemical, mechanical, electrical, and optical properties) to the nanomaterials. Because of these intriguing features, CNs have been extensively employed in biosensor applications. In particular, carbon nanotubes (CNTs), nanodiamonds, graphene, and fullerenes serve as scaffolds for the immobilization of biomolecules at their surface and are also used as transducers for the conversion of signals associated with the recognition of biological analytes. Herein, we provide a comprehensive review on the synthesis of CNs and their potential application to biosensors. In addition, we discuss the efforts to improve the mechanical and electrical properties of biosensors by combining different CNs.

scholarly journals The Effect of Semiconductor Morphology on the Spatial Resolution of ZnO Based Light-Addressable Potentiometric Sensors

Proceedings ◽  
2018 ◽  
Vol 2 (13) ◽  
pp. 917
Author(s):  
Ying Tu ◽  
Jianwei Li ◽  
De-Wen Zhang ◽  
Joe Briscoe ◽  
Steffi Krause
Keyword(s):  
Spatial Resolution ◽  
Chemical Vapour Deposition ◽  
Vapour Deposition ◽  
Chemical Vapour ◽  
Potentiometric Sensors ◽  
Zno Films ◽  
Semiconductor Structures ◽  
Electrical Potentials ◽  
Biological Analytes

Light-addressable potentiometric sensors (LAPS) measure ac photocurrent at electrolyte-insulator-semiconductor (EIS) and, more recently, electrolyte-semiconductor structures to produce spatiotemporal images of chemical or biological analytes, electrical potentials and impedance. One of the most important properties for LAPS is spatial resolution, which determines the smallest features that can be resolved in LAPS images. In this work, the use of nanostructured ZnO for LAPS was explored. The effect of ZnO morphology on the spatial resolution was studied with a LAPS setup. The best resolution of 2 µm was achieved in ZnO films produced by aerosol-assisted chemical vapour deposition (AACVD).

Surface Micromachined Dielectrophoretic Gates for the Front-End Device of a Biodetection System

2005 ◽  
Vol 128 (1) ◽  
pp. 14-19 ◽  
Author(s):  
Conrad D. James ◽  
Murat Okandan ◽  
Paul Galambos ◽  
Seethambal S. Mani ◽  
Dawn Bennett ◽  
...  
Keyword(s):  
Phase Separation ◽  
Radio Frequency ◽  
Fluorescent Labeling ◽  
Frequency Range ◽  
Flowing Fluid ◽  
Separation Device ◽  
Air Samples ◽  
Biological Particles ◽  
Front End ◽  
Biological Analytes

We present a novel separation device for the front-end of a biodetection system to discriminate between biological and non-biological analytes captured in air samples. By combining AC dielectrophoresis along the flow streamlines and a field-induced phase-separation, the device utilizes “dielectrophoretic gating”to separate analytes suspended in a flowing fluid based on their intrinsic polarizability properties. The gates are integrated into batch fabricated self-sealed surface-micromachined fluid channels. We demonstrate that setting the gate to a moderate voltage in the radio frequency range removed bacteria cells from a mixture containing non-biological particles without the need for fluorescent labeling or antibody-antigen hybridization, and also validate experimentally basic relations for estimating the gate performance.

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