Detection of biological analytes using surface plasmon resonance as a biosensing technique for possible development of a point of care diagnostic tool

2019 ◽  
Author(s):  
Rudzani Malabi ◽  
Sello L. Manoto ◽  
Saturnin Ombinda-Lemboumba ◽  
Malik Maaza ◽  
Patience Mthunzi-Kufa
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Diagnostic Tool ◽  
Point Of Care ◽  
Biological Analytes

scholarly journals New portable smartphone-based PDMS microfluidic kit for the simultaneous colorimetric detection of arsenic and mercury

RSC Advances ◽  
2018 ◽  
Vol 8 (48) ◽  
pp. 27091-27100 ◽  
Author(s):  
Abbas Motalebizadeh ◽  
Hasan Bagheri ◽  
Sasan Asiaei ◽  
Nasim Fekrat ◽  
Abbas Afkhami
Keyword(s):  
Gold Nanoparticles ◽  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Point Of Care ◽  
Colorimetric Detection ◽  
Microfluidic Platform

A smartphone-based microfluidic platform was developed for point-of-care (POC) detection using surface plasmon resonance (SPR) of gold nanoparticles (GNPs).

Surface plasmon resonance application in prostate cancer biomarker research

2015 ◽  
Vol 69 (1) ◽  
Author(s):  
Pavel Damborský ◽  
Narayanan Madaboosi ◽  
Virginia Chu ◽  
João P. Conde ◽  
Jaroslav Katrlík
Keyword(s):  
Prostate Cancer ◽  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Point Of Care ◽  
Specific Antigen ◽  
Binding Affinities ◽  
Complex Protein ◽  
Microfluidic Immunoassay ◽  
Biomarker Research

AbstractProstate cancer (PCa) diagnostics can be effectively addressed using sensor-based approaches. Proper selection of biomarkers to be included in biosensors for accurate detection becomes the need of the hour. Such biosensor and biochip technologies enable fast and efficient determination of proteins and provide a remarkable insight into the changes in the protein structure, such as aberrant glycosylation, which can increase the performance, sensitivity and specificity of clinic assays. However, for a thorough comprehension of such complex protein modifications, it is crucial to understand their biospecific interactions. Surface plasmon resonance (SPR), one of the most rapidly developing techniques for measuring real-time quantitative binding affinities and kinetics of the interactions of antigens and antibodies, was chosen as an appropriate tool for this purpose. Herein, experiments on the interactions of antibodies specific against different epitopes of free and complexed prostate-specific antigen (PSA), a prominent PCa biomarker, are presented with two main aims: (i) to continue as lectin glycoprofiling studies and; (ii) to be used in microfluidic immunoassay-based platforms for point-of-care devices. Various PSA-specific antibodies were covalently immobilized on the biochip surface via amine coupling, and free or complexed PSA was injected into the dual-flow channels of the SPR device. Kinetic parameters and affinity constants of these interactions, as well as cross-reactivities of the used antibodies were determined. The sandwich assay for PSA determination was developed employing both primary and secondary anti-PSA antibodies. Sensitivity of the assay was 3.63 nM

A Rapid and Quantitative Serum Test for SARS-CoV-2 Antibodies with Portable Surface Plasmon Resonance Sensing

2020 ◽  
Author(s):  
Abdelhadi Djaileb ◽  
Benjamin Charron ◽  
Maryam Hojjat Jodaylami ◽  
Vincent Thibault ◽  
Julien Coutu ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Human Serum ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Point Of Care ◽  
The Novel ◽  
Label Free ◽  
Spr Sensor ◽  
Novel Coronavirus ◽  
Nanomolar Range

We report a surface plasmon resonance (SPR) sensor detecting nucleocapsid antibodies specific against the novel coronavirus 2019 (SARS-CoV-2) in undiluted human serum. When exposed to SARS-CoV-2, the immune system responds by expressing antibodies at levels that can be detected and monitored to identify the patient population immunized against SARD-CoV-2 and support efforts to deploy a vaccine strategically. A SPR sensor coated with a peptide monolayer and functionalized with SARS-CoV-2 nucleocapsid recombinant protein detected anti-SARS-CoV-2 antibodies in the nanomolar range. This bioassay was performed on a portable SPR instrument in undiluted human serum and results were collected within 15 minutes of sample/sensor contact. This strategy paves the way to point-of-care and label-free rapid testing for antibodies.

A Rapid and Quantitative Serum Test for SARS-CoV-2 Antibodies with Portable Surface Plasmon Resonance Sensing

2020 ◽  
Author(s):  
Abdelhadi Djaileb ◽  
Benjamin Charron ◽  
Maryam Hojjat Jodaylami ◽  
Vincent Thibault ◽  
Julien Coutu ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Human Serum ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Point Of Care ◽  
The Novel ◽  
Label Free ◽  
Spr Sensor ◽  
Novel Coronavirus ◽  
Nanomolar Range

We report a surface plasmon resonance (SPR) sensor detecting nucleocapsid antibodies specific against the novel coronavirus 2019 (SARS-CoV-2) in undiluted human serum. When exposed to SARS-CoV-2, the immune system responds by expressing antibodies at levels that can be detected and monitored to identify the patient population immunized against SARD-CoV-2 and support efforts to deploy a vaccine strategically. A SPR sensor coated with a peptide monolayer and functionalized with SARS-CoV-2 nucleocapsid recombinant protein detected anti-SARS-CoV-2 antibodies in the nanomolar range. This bioassay was performed on a portable SPR instrument in undiluted human serum and results were collected within 15 minutes of sample/sensor contact. This strategy paves the way to point-of-care and label-free rapid testing for antibodies.

Development of Localized Surface Plasmon Resonance-Based Point-of-Care System

Plasmonics ◽  
2014 ◽  
Vol 9 (4) ◽  
pp. 835-844 ◽  
Author(s):  
Xiaodong Zhou ◽  
Ten It Wong ◽  
Hong Yan Song ◽  
Lin Wu ◽  
Yi Wang ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Localized Surface Plasmon Resonance ◽  
Point Of Care ◽  
Localized Surface Plasmon ◽  
Point Of Care System ◽  
Care System

scholarly journals Point-of-Care Surface Plasmon Resonance Biosensor for Stroke Biomarkers NT-proBNP and S100β Using a Functionalized Gold Chip with Specific Antibody

Sensors ◽  
2019 ◽  
Vol 19 (11) ◽  
pp. 2533 ◽  
Author(s):  
Dorin Harpaz ◽  
Brescia Koh ◽  
Robert S. Marks ◽  
Raymond C.S. Seet ◽  
Ibrahim Abdulhalim ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Limit Of Detection ◽  
Close Contact ◽  
Point Of Care ◽  
Plasma Samples ◽  
Analyte Concentration ◽  
Free Electrons ◽  
Specific Detection

Surface-plasmon-resonance (SPR) is a quantum-electromagnetic phenomenon arising from the interaction of light with free electrons at a metal-dielectric interface. At a specific angle/wavelength of light, the photon’s energy is transferred to excite the oscillation of the free electrons on the surface. A change in the refractive-index (RI) may occur, which is influenced by the analyte concentration in the medium in close contact with the metal surface. SPR has been widely used for the detection of gaseous, liquid, or solid samples. In this study, a functionalized specific SPR chip was designed and used in a novel point-of-care SPR module (PhotonicSys SPR H5) for the detection of the stroke biomarkers NT-proBNP and S100β. These biomarkers have proven to be good for stroke diagnosis, with sensitivity and specificity of >85%. Specific detection was done by binding a biomolecular-recognizing antibody onto the Au SPR-chip. Detection was tested in water and plasma samples. NT-proBNP and S100β were detected in a range of concentrations for stroke, from 0.1 ng/mL to 10 ng/mL. The RI of the blank plasma samples was 1.362412, and the lowest concentration tested for both biomarkers showed a prominent shift in the RI signal (0.25 ng/mL NT-proBNP (1.364215) and S100β (1.364024)). The sensor demonstrated a clinically relevant limit-of-detection of less than ng/mL.

Sign in / Sign up

Export Citation Format

Share Document