scholarly journals Characterization of corneal stromal stem cells with the potential for epithelial transdifferentiation

2013 ◽  
Vol 4 (3) ◽  
pp. 75 ◽  
Author(s):  
Khurram Hashmani ◽  
Matthew Branch ◽  
Laura Sidney ◽  
Permesh Dhillon ◽  
Megha Verma ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stromal Stem Cells

scholarly journals Microfluidic Tools for Enhanced Characterization of Therapeutic Stem Cells and Prediction of Their Potential Antimicrobial Secretome

Antibiotics ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 750
Author(s):  
Pasquale Marrazzo ◽  
Valeria Pizzuti ◽  
Silvia Zia ◽  
Azzurra Sargenti ◽  
Daniele Gazzola ◽  
...  
Keyword(s):  
Stem Cells ◽  
Defense Mechanisms ◽  
Live Cells ◽  
Label Free ◽  
Bacterial Diseases ◽  
Prospective Application ◽  
Therapy Strategies ◽  
Stromal Stem Cells ◽  
Different Sources

Antibiotic resistance is creating enormous attention on the development of new antibiotic-free therapy strategies for bacterial diseases. Mesenchymal stromal stem cells (MSCs) are the most promising candidates in current clinical trials and included in several cell-therapy protocols. Together with the well-known immunomodulatory and regenerative potential of the MSC secretome, these cells have shown direct and indirect anti-bacterial effects. However, the low reproducibility and standardization of MSCs from different sources are the current limitations prior to the purification of cell-free secreted antimicrobial peptides and exosomes. In order to improve MSC characterization, novel label-free functional tests, evaluating the biophysical properties of the cells, will be advantageous for their cell profiling, population sorting, and quality control. We discuss the potential of emerging microfluidic technologies providing new insights into density, shape, and size of live cells, starting from heterogeneous or 3D cultured samples. The prospective application of these technologies to studying MSC populations may contribute to developing new biopharmaceutical strategies with a view to naturally overcoming bacterial defense mechanisms.

840 DIFFERENTIAL ISOLATION AND SURFACE MARKER CHARACTERIZATION OF EPITHELIAL AND STROMAL STEM CELLS IN BENIGN PROSTATE TISSUE

2011 ◽  
Vol 10 (2) ◽  
pp. 266
Author(s):  
N. Neli ◽  
J. Hennenlotter ◽  
C. Schwentner ◽  
T. Todenhoefer ◽  
S. Grimm ◽  
...  
Keyword(s):  
Stem Cells ◽  
Surface Marker ◽  
Prostate Tissue ◽  
Stromal Stem Cells ◽  
Benign Prostate

scholarly journals Isolation, Characterization, Differentiation and Immunomodulatory Capacity of Mesenchymal Stromal/Stem Cells from Human Perirenal Adipose Tissue

Cells ◽  
2019 ◽  
Vol 8 (11) ◽  
pp. 1346 ◽  
Author(s):  
Baer ◽  
Koch ◽  
Hickmann ◽  
Schubert ◽  
Cinatl ◽  
...  
Keyword(s):  
Stem Cells ◽  
Adipose Tissue ◽  
Epithelial Cells ◽  
Lipoteichoic Acid ◽  
Differentiation Potential ◽  
Perivascular Niche ◽  
Perirenal Adipose Tissue ◽  
Multipotent Cells ◽  
Stromal Stem Cells

Mesenchymal stromal/stem cells (MSCs) are immature multipotent cells, which represent a rare population in the perivascular niche within nearly all tissues. The most abundant source to isolate MSCs is adipose tissue. Currently, perirenal adipose tissue is rarely described as the source of MSCs. MSCs were isolated from perirenal adipose tissue (prASCs) from patients undergoing tumor nephrectomies, cultured and characterized by flow cytometry and their differentiation potential into adipocytes, chondrocytes, osteoblasts and epithelial cells. Furthermore, prASCs were stimulated with lipopolysaccharide (LPS), lipoteichoic acid (LTA) or a mixture of cytokines (cytomix). In addition, prASC susceptibility to human cytomegalovirus (HCMV) was investigated. The expression of inflammatory readouts was estimated by qPCR and immunoassay. HCMV infection was analyzed by qPCR and immunostaining. Characterization of cultured prASCs shows the cells meet the criteria of MSCs and prASCs can undergo trilineage differentiation. Cultured prASCs can be induced to differentiate into epithelial cells, shown by cytokeratin 18 expression. Stimulation of prASCs with LPS or cytomix suggests the cells are capable of initiating an inflammation-like response upon stimulation with LPS or cytokines, whereas, LTA did not induce a significant effect on the readouts (ICAM-1, IL-6, TNFα, MCP-1 mRNA and IL-6 protein). HCMV broadly infects prASCs, showing a viral load dependent cytopathological effect (CPE). Our current study summarizes the isolation and culture of prASCs, clearly characterizes the cells, and demonstrates their immunomodulatory potential and high permissiveness for HCMV.

Comprehensive Phenotypic Characterization of Human Adipose-Derived Stromal/Stem Cells and Their Subsets by a High Throughput Technology

2013 ◽  
Vol 22 (2) ◽  
pp. 330-339 ◽  
Author(s):  
Patrick C. Baer ◽  
Selim Kuçi ◽  
Michael Krause ◽  
Zyrafete Kuçi ◽  
Stefan Zielen ◽  
...  
Keyword(s):  
Stem Cells ◽  
High Throughput ◽  
Phenotypic Characterization ◽  
High Throughput Technology ◽  
Stromal Stem Cells

Characterization of lung resident-mesenchymal stromal/stem cells from paediatric donors

2019 ◽  
Author(s):  
Melanie Brügger ◽  
Thomas Démoulins ◽  
Beatrice Zumkehr ◽  
Ueli Moehrlen ◽  
Thomas M. Marti ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stromal Stem Cells

scholarly journals Characterization of Extracellular Vesicles from Preconditioned Human Adipose-Derived Stromal/Stem Cells

2021 ◽  
Vol 22 (6) ◽  
pp. 2873
Author(s):  
Alec Geßner ◽  
Benjamin Koch ◽  
Kevin Klann ◽  
Dominik C. Fuhrmann ◽  
Samira Farmand ◽  
...  
Keyword(s):  
Stem Cells ◽  
Epithelial Cells ◽  
Extracellular Vesicles ◽  
Therapeutic Option ◽  
Culture Conditions ◽  
Oxidative Potential ◽  
Organ Regeneration ◽  
Stromal Stem Cells

Cell-free therapy using extracellular vesicles (EVs) from adipose-derived mesenchymal stromal/stem cells (ASCs) seems to be a safe and effective therapeutic option to support tissue and organ regeneration. The application of EVs requires particles with a maximum regenerative capability and hypoxic culture conditions as an in vitro preconditioning regimen has been shown to alter the molecular composition of released EVs. Nevertheless, the EV cargo after hypoxic preconditioning has not yet been comprehensively examined. The aim of the present study was the characterization of EVs from hypoxic preconditioned ASCs. We investigated the EV proteome and their effects on renal tubular epithelial cells in vitro. While no effect of hypoxia was observed on the number of released EVs and their protein content, the cargo of the proteins was altered. Proteomic analysis showed 41 increased or decreased proteins, 11 in a statistically significant manner. Furthermore, the uptake of EVs in epithelial cells and a positive effect on oxidative stress in vitro were observed. In conclusion, culture of ASCs under hypoxic conditions was demonstrated to be a promising in vitro preconditioning regimen, which alters the protein cargo and increases the anti-oxidative potential of EVs. These properties may provide new potential therapeutic options for regenerative medicine.

scholarly journals Evolution of ASC Immunophenotypical Subsets During Expansion In Vitro

2020 ◽  
Vol 21 (4) ◽  
pp. 1408 ◽  
Author(s):  
Qiuyue Peng ◽  
Hiva Alipour ◽  
Simone Porsborg ◽  
Trine Fink ◽  
Vladimir Zachar
Keyword(s):  
Stem Cells ◽  
Early Stage ◽  
Clinical Use ◽  
Cross Sectional ◽  
Full Characterization ◽  
Surface Molecules ◽  
Surface Marker Expression ◽  
Stromal Stem Cells

Adipose-derived stromal/stem cells (ASCs) are currently being considered for clinical use for a number of indications. In order to develop standardized clinical protocols, it is paramount to have a full characterization of the stem cell preparations. The surface marker expression of ASCs has previously been characterized in multiple studies. However, most of these studies have provided a cross-sectional description of ASCs in either earlier or later passages. In this study, we evaluate the dynamic changes of 15 different surface molecules during culture. Using multichromatic flow cytometry, ASCs from three different donors each in passages 1, 2, 4, 6, and 8 were analyzed for their co-expression of markers associated with mesenchymal stem cells, wound healing, immune regulation, ASC markers, and differentiation capacity, respectively. We confirmed that at an early stage, ASC displayed a high heterogeneity with a plethora of subpopulations, which by culturing became more homogeneous. After a few passages, virtually all ASCs expressed CD29, CD166 and CD201, in addition to canonical markers CD73, CD90, and CD105. However, even at passage 8, there were several predominant lineages that differed with respect to the expression of CD34, CD200 and CD271. Although the significance of remaining subpopulations still needs to be elucidated, our results underscore the necessity to fully characterize ASCs prior to clinical use.

Characterization of Canine Adipose-Derived Mesenchymal Stromal/Stem Cells in Serum-Free Medium

2018 ◽  
Vol 24 (7) ◽  
pp. 399-411 ◽  
Author(s):  
Zhuoming Liu ◽  
Rudell Screven ◽  
Lynne Boxer ◽  
Michael J. Myers ◽  
Lax R. Devireddy
Keyword(s):  
Stem Cells ◽  
Serum Free Medium ◽  
Free Medium ◽  
Serum Free ◽  
Stromal Stem Cells

Establishment and Characterization of Bone Marrow Mesenchymal Stromal/Stem Cells (MSCs) Derived from α-1,3-Galactosyltransferase Knock Out(GalT KO) Pig

2013 ◽  
Vol 28 (3) ◽  
pp. 281-287
Author(s):  
Sun-A Ock ◽  
◽  
Keon Bong Oh ◽  
Seongsoo Hwang ◽  
Seoki Im ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Knock Out ◽  
Stromal Stem Cells
Sign in / Sign up

Export Citation Format

Share Document