Comprehensive Phenotypic Characterization of Human Adipose-Derived Stromal/Stem Cells and Their Subsets by a High Throughput Technology

2013 ◽  
Vol 22 (2) ◽  
pp. 330-339 ◽  
Author(s):  
Patrick C. Baer ◽  
Selim Kuçi ◽  
Michael Krause ◽  
Zyrafete Kuçi ◽  
Stefan Zielen ◽  
...  
Keyword(s):  
Stem Cells ◽  
High Throughput ◽  
Phenotypic Characterization ◽  
High Throughput Technology ◽  
Stromal Stem Cells

scholarly journals Microfluidic Tools for Enhanced Characterization of Therapeutic Stem Cells and Prediction of Their Potential Antimicrobial Secretome

Antibiotics ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 750
Author(s):  
Pasquale Marrazzo ◽  
Valeria Pizzuti ◽  
Silvia Zia ◽  
Azzurra Sargenti ◽  
Daniele Gazzola ◽  
...  
Keyword(s):  
Stem Cells ◽  
Defense Mechanisms ◽  
Live Cells ◽  
Label Free ◽  
Bacterial Diseases ◽  
Prospective Application ◽  
Therapy Strategies ◽  
Stromal Stem Cells ◽  
Different Sources

Antibiotic resistance is creating enormous attention on the development of new antibiotic-free therapy strategies for bacterial diseases. Mesenchymal stromal stem cells (MSCs) are the most promising candidates in current clinical trials and included in several cell-therapy protocols. Together with the well-known immunomodulatory and regenerative potential of the MSC secretome, these cells have shown direct and indirect anti-bacterial effects. However, the low reproducibility and standardization of MSCs from different sources are the current limitations prior to the purification of cell-free secreted antimicrobial peptides and exosomes. In order to improve MSC characterization, novel label-free functional tests, evaluating the biophysical properties of the cells, will be advantageous for their cell profiling, population sorting, and quality control. We discuss the potential of emerging microfluidic technologies providing new insights into density, shape, and size of live cells, starting from heterogeneous or 3D cultured samples. The prospective application of these technologies to studying MSC populations may contribute to developing new biopharmaceutical strategies with a view to naturally overcoming bacterial defense mechanisms.

840 DIFFERENTIAL ISOLATION AND SURFACE MARKER CHARACTERIZATION OF EPITHELIAL AND STROMAL STEM CELLS IN BENIGN PROSTATE TISSUE

2011 ◽  
Vol 10 (2) ◽  
pp. 266
Author(s):  
N. Neli ◽  
J. Hennenlotter ◽  
C. Schwentner ◽  
T. Todenhoefer ◽  
S. Grimm ◽  
...  
Keyword(s):  
Stem Cells ◽  
Surface Marker ◽  
Prostate Tissue ◽  
Stromal Stem Cells ◽  
Benign Prostate

scholarly journals Isolation, Characterization, Differentiation and Immunomodulatory Capacity of Mesenchymal Stromal/Stem Cells from Human Perirenal Adipose Tissue

Cells ◽  
2019 ◽  
Vol 8 (11) ◽  
pp. 1346 ◽  
Author(s):  
Baer ◽  
Koch ◽  
Hickmann ◽  
Schubert ◽  
Cinatl ◽  
...  
Keyword(s):  
Stem Cells ◽  
Adipose Tissue ◽  
Epithelial Cells ◽  
Lipoteichoic Acid ◽  
Differentiation Potential ◽  
Perivascular Niche ◽  
Perirenal Adipose Tissue ◽  
Multipotent Cells ◽  
Stromal Stem Cells

Mesenchymal stromal/stem cells (MSCs) are immature multipotent cells, which represent a rare population in the perivascular niche within nearly all tissues. The most abundant source to isolate MSCs is adipose tissue. Currently, perirenal adipose tissue is rarely described as the source of MSCs. MSCs were isolated from perirenal adipose tissue (prASCs) from patients undergoing tumor nephrectomies, cultured and characterized by flow cytometry and their differentiation potential into adipocytes, chondrocytes, osteoblasts and epithelial cells. Furthermore, prASCs were stimulated with lipopolysaccharide (LPS), lipoteichoic acid (LTA) or a mixture of cytokines (cytomix). In addition, prASC susceptibility to human cytomegalovirus (HCMV) was investigated. The expression of inflammatory readouts was estimated by qPCR and immunoassay. HCMV infection was analyzed by qPCR and immunostaining. Characterization of cultured prASCs shows the cells meet the criteria of MSCs and prASCs can undergo trilineage differentiation. Cultured prASCs can be induced to differentiate into epithelial cells, shown by cytokeratin 18 expression. Stimulation of prASCs with LPS or cytomix suggests the cells are capable of initiating an inflammation-like response upon stimulation with LPS or cytokines, whereas, LTA did not induce a significant effect on the readouts (ICAM-1, IL-6, TNFα, MCP-1 mRNA and IL-6 protein). HCMV broadly infects prASCs, showing a viral load dependent cytopathological effect (CPE). Our current study summarizes the isolation and culture of prASCs, clearly characterizes the cells, and demonstrates their immunomodulatory potential and high permissiveness for HCMV.

Site- and structure-specific characterization ofN-glycoprotein markers of MCF-7 cancer stem cells using isotopic-labelling quantitativeN-glycoproteomics

2019 ◽  
Vol 55 (55) ◽  
pp. 7934-7937 ◽  
Author(s):  
Yue Wang ◽  
Feifei Xu ◽  
Kaijie Xiao ◽  
Yun Chen ◽  
Zhixin Tian
Keyword(s):  
Stem Cells ◽  
Cancer Stem Cells ◽  
High Throughput ◽  
Isotopic Labelling ◽  
Proteome Level ◽  
Mcf 7

High-throughput proteome-level characterization of stemness markers of MCF-7 cancer stem cells was carried out using our recently developed site- and structure-specific isotopic-labelled quantitativeN-glycoproteomics pipeline.

scholarly journals Characterization of corneal stromal stem cells with the potential for epithelial transdifferentiation

2013 ◽  
Vol 4 (3) ◽  
pp. 75 ◽  
Author(s):  
Khurram Hashmani ◽  
Matthew Branch ◽  
Laura Sidney ◽  
Permesh Dhillon ◽  
Megha Verma ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stromal Stem Cells

scholarly journals Brain tumor stem cells: phenotypic characterization and directed therapeutic approaches

2015 ◽  
Vol 3 (2) ◽  
pp. 177-183
Author(s):  
L. Belska ◽  
M. Lisyany
Keyword(s):  
Stem Cells ◽  
Brain Tumor ◽  
Intracellular Signaling ◽  
Phenotypic Characterization ◽  
Tumor Stem Cells ◽  
Therapeutic Approaches ◽  
Brain Tumor Stem Cells ◽  
The Brain ◽  
Methods Of Isolation

The review presents the current conceptions of the origin, methods of isolation and phenotypic characterization of the brain tumor stem cells. Phenotypic similarity in molecular markers between cancer and neural stem cells is shown. Therapeutic approaches of impact on the brain tumor stem cells and on the intracellular signaling pathways of cancer stem cells are described.

scholarly journals High-Throughput Phenotypic Characterization of Pseudomonas aeruginosa Membrane Transport Genes

PLoS Genetics ◽  
2008 ◽  
Vol 4 (10) ◽  
pp. e1000211 ◽  
Author(s):  
Daniel A. Johnson ◽  
Sasha G. Tetu ◽  
Katherine Phillippy ◽  
Joan Chen ◽  
Qinghu Ren ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Membrane Transport ◽  
High Throughput ◽  
Phenotypic Characterization
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