scholarly journals Methodological Problems in Whole-Body Autoradiography of Rainbow Trout

1979 ◽  
Vol 20 (4) ◽  
pp. 604-606
Author(s):  
Kristian Ingebrigtsen ◽  
Torill Bergsjø
1990 ◽  
Vol 14 (1) ◽  
pp. 84-87 ◽  
Author(s):  
WILLIAM J. WADDELL ◽  
JOHN J. LECH ◽  
CAROLYN MARLOWE ◽  
KEVIN M. KLEINOW ◽  
MARVIN A. FRIEDMAN

1988 ◽  
Vol 254 (6) ◽  
pp. R891-R896 ◽  
Author(s):  
F. P. Lafeber ◽  
G. Flik ◽  
S. E. Wendelaar Bonga ◽  
S. F. Perry

Bidirectional whole body flux and branchial Ca2+ influx were measured in freshwater rainbow trout. Intra-arterial injections of homogenates of Stannius corpuscles (CS) as well as of a 54-kDa isolated product (hypocalcin) exerted an inhibitory effect on whole body Ca2+ influx, but did not effect Ca2+ efflux. Hypocalcin was more effective in reducing Ca2+ influx in trout acclimated to low-calcium freshwater than in fish from normal-calcium water. We conclude that the isolated product (hypocalcin) represents the hypocalcemic principle of the CS. Similar doses of hypocalcin caused quantitatively similar decreases in Ca2+ influx in vivo and in the isolated perfused head preparation. This indicates that the gills form the principle target for hypocalcin in trout. The branchial transepithelial potential did not change during hormone treatments. Possible mechanisms of hypocalcin action are suggested.


1980 ◽  
Vol 13 (2) ◽  
pp. 202-210 ◽  
Author(s):  
MASAHITO WATANABE ◽  
TAKASHI KIHARA ◽  
MASAHISA SHIMADA ◽  
KIYOHISA KURIMOTO

1991 ◽  
Vol 48 (4) ◽  
pp. 583-590 ◽  
Author(s):  
Paul W. Webb

Routine swimming encompasses all volitional motions of fish. It is usually assumed to be quasi-steady, i.e. routine swimming is mechanically equivalent to steady swimming. Routine swimming of rainbow trout, Oncorhynchus mykiss, was dominated by unsteady motions of linear and centripetal (angular) acceleration. Constant-speed swimming was rare. Mean speeds and acceleration rates were small. Tail-beat frequencies were nevertheless strongly correlated with mean swimming speed, but increased more rapidly with increasing speed in routine swimming than in steady swimming. Tail-beat amplitudes and propulsive wavelengths were similar to values seen in steady swimming. The composition of routine swimming and analysis of the force balance showed that routine swimming was not quasi-steady. Therefore, forces and rates of working should be estimated from a complete description of whole-body deformation. This is impractical. Drag dominated resistance in routine swimming, such that average thrust (= resistance) may be computed from mean speed and/or averaged kinematic variables for the trailing edge with a correction factor of approximately 3. Analysis of routine swimming may permit comparisons among a wider range of vertebrates than possible with commonly used methods.


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