scholarly journals Gene expression profiling in whole-blood samples from postmenopausal women exposed to hormone replacement therapy

2005 ◽  
Vol 7 (S2) ◽  
Author(s):  
V Dumeaux ◽  
J Johansen ◽  
AL Børresen-Dale ◽  
E Lund
Keyword(s):  
Gene Expression ◽  
Hormone Replacement Therapy ◽  
Gene Expression Profiling ◽  
Replacement Therapy ◽  
Postmenopausal Women ◽  
Whole Blood ◽  
Expression Profiling ◽  
Hormone Replacement ◽  
Blood Samples ◽  
Whole Blood Samples

Gene expression profiling in human whole blood samples after controlled testosterone application and exercise

2011 ◽  
Vol 3 (10) ◽  
pp. 652-660 ◽  
Author(s):  
Martin Schönfelder ◽  
Hande Hofmann ◽  
Patricia Anielski ◽  
Detlef Thieme ◽  
Renate Oberhoffer ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Expression Profiling ◽  
Whole Blood ◽  
Expression Profiling ◽  
Blood Samples ◽  
Human Whole Blood ◽  
Whole Blood Samples

scholarly journals Gene expression profiling of human whole blood samples with the Illumina WG-DASL assay

BMC Genomics ◽  
2011 ◽  
Vol 12 (1) ◽  
Author(s):  
Mary E Winn ◽  
Marian Shaw ◽  
Craig April ◽  
Brandy Klotzle ◽  
Jian-Bing Fan ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Expression Profiling ◽  
Whole Blood ◽  
Expression Profiling ◽  
Blood Samples ◽  
Human Whole Blood ◽  
Whole Blood Samples

scholarly journals Hormone replacement therapy dependent changes in breast cancer-related gene expression in breast tissue of healthy postmenopausal women

2011 ◽  
Vol 5 (6) ◽  
pp. 504-516 ◽  
Author(s):  
Anieta M. Sieuwerts ◽  
Giuseppina De Napoli ◽  
Anne van Galen ◽  
Helenius J. Kloosterboer ◽  
Vanja de Weerd ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Gene Expression ◽  
Hormone Replacement Therapy ◽  
Replacement Therapy ◽  
Postmenopausal Women ◽  
Breast Tissue ◽  
Hormone Replacement ◽  
Related Gene ◽  
Cancer Related Gene

scholarly journals Influence of hormone replacement therapy on eccentric exercise induced myogenic gene expression in postmenopausal women

2009 ◽  
Vol 107 (5) ◽  
pp. 1381-1388 ◽  
Author(s):  
Christina M. Dieli-Conwright ◽  
Tanya M. Spektor ◽  
Judd C. Rice ◽  
Fred R. Sattler ◽  
E. Todd Schroeder
Keyword(s):  
Gene Expression ◽  
Hormone Replacement Therapy ◽  
Mrna Expression ◽  
Replacement Therapy ◽  
Postmenopausal Women ◽  
Eccentric Exercise ◽  
Hormone Replacement ◽  
Control Group ◽  
Regulatory Factor ◽  
Myogenic Regulatory Factor

Hormone replacement therapy (HRT) is used in postmenopausal women to relieve symptoms of menopause and prevent osteoporosis. We sought to evaluate changes in mRNA expression of key myogenic factors in postmenopausal women taking and not taking HRT following a high-intensity eccentric resistance exercise. Fourteen postmenopausal women were studied and included 6 control women not using HRT (59 ± 4 years, 63 ± 17 kg) and 8 women using traditional HRT (59 ± 4 yr, 89 ± 24 kg). Both groups performed 10 sets of 10 maximal eccentric repetitions of single-leg extension on a Cybex dynamometer at 60°/s. Muscle biopsies of the vastus lateralis were obtained from the exercised leg at baseline and 4 h after the exercise bout. Gene expression was determined using RT-PCR for follistatin, forkhead box 3A (FOXO3A), muscle atrophy F-box (MAFbx), muscle ring finger-1 (MuRF-1), myogenic differentiation factor (MyoD), myogenin, myostatin, myogenic factor 5 (Myf5), and muscle regulatory factor 4 (MRF4). At rest, the HRT group expressed higher levels of MyoD, myogenin, Myf5, MRF4, and follistatin ( P < 0.05). In response to eccentric exercise, follistatin, MyoD, myogenin, Myf5, and MRF4 were significantly increased ( P ≤ 0.05) and FOXO3A, MAFbx, MuRF-1, and myostatin were significantly decreased in the control and HRT groups ( P ≤ 0.05). Significantly greater changes in mRNA expression of follistatin, FOXO3A, MAFbx, MuRF-1, MyoD, myogenin, myostatin, Myf5, and MRF4 (p≤0.05) occurred in the HRT group than in the control group after exercise. These data suggest that postmenopausal women using HRT express higher myogenic regulatory factor gene expression, which may reflect an attempt to preserve muscle mass. Furthermore, postmenopausal women using HRT experienced a greater myogenic response to maximal eccentric exercise.

scholarly journals Analysis of the Influence of Hormone Replacement Therapy on Osteocalcin Gene Expression in Postmenopausal Women

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Mansur Rahnama ◽  
Izabela Jastrzębska-Jamrogiewicz ◽  
Rafał Jamrogiewicz ◽  
Grzegorz Trybek
Keyword(s):  
Gene Expression ◽  
Hormone Replacement Therapy ◽  
Replacement Therapy ◽  
Bone Metabolism ◽  
Postmenopausal Women ◽  
Bone Mineralization ◽  
Hormone Replacement ◽  
Peripheral Blood Lymphocytes ◽  
Local Bone ◽  
Osteocalcin Gene

Background.Osteocalcin (OC) contributes to the process of bone mineralization. Present study was designed to investigate the changes in OC gene expression of postmenopausal women treated with hormone replacement therapy (HRT). Study was also designed to evaluate OC gene expression in cells which are not part of connective tissue.Material and Methods.Research was carried out on 30 postmenopausal women not treated and 30 treated with HRT. Examination of OC gene expression was conducted on peripheral blood lymphocytes (PBL) and buccal epithelial lining (BEL). Densitometry was conducted on femur and mandible.Results.Tests revealed OC gene expression in BEL and PBL. BMD was higher in groups treated with HRT. Assessment of correlation between the OC gene expression in BEL and BMD of mandible revealed significant positive relation.Conclusions.OC gene expression can be stated BEL and PBL. Analysis of correlation between OC gene expression in oral cavity and mandible BMD showed significant correlation between local OC expression and local bone metabolism. The relation between OC gene expression and bone metabolism is complex and further research is needed to clear all of the uncertainties.

Effects of oral combined hormone replacement therapy on platelet aggregation in postmenopausal women

2001 ◽  
Vol 100 (2) ◽  
pp. 207-213 ◽  
Author(s):  
Helena J. TEEDE ◽  
Barry P. MCGRATH ◽  
Alan TURNER ◽  
Harry MAJEWSKI
Keyword(s):  
Nitric Oxide ◽  
Arachidonic Acid ◽  
Placebo Group ◽  
Hormone Replacement Therapy ◽  
Platelet Aggregation ◽  
Replacement Therapy ◽  
Postmenopausal Women ◽  
Whole Blood ◽  
Hormone Replacement ◽  
Impedance Aggregometry

The effects of combined oestrogen/progestin hormone replacement therapy (HRT) on platelet aggregation were studied using women on HRT or placebo. The study involved 32 postmenopausal women (aged 50–75 years) who were enrolled in a double-blind randomized controlled trial, and who received either oral continuous combined HRT (Kliogest®; 2 mg of oestradiol+1 mg of norethisterone) or placebo for a minimum of 6 months. Platelet aggregation was measured by whole-blood impedance aggregometry in response to the agonists collagen, arachidonic acid and ADP. To determine whether the effects of oestrogen on platelets were influenced by platelet-derived nitric oxide, exposure to collagen was repeated in the presence of the nitric oxide synthase inhibitor NG-monomethyl-L-arginine (L-NMMA). Mean platelet volume was similar in the two groups. Compared with the placebo group, the women on HRT had similar rates and maximum values of platelet aggregation in response to collagen, arachidonic acid and ADP. Addition of L-NMMA did not alter the aggregation response to collagen in either the HRT or the placebo group. In conclusion, postmenopausal women on oral combined continuous HRT comprising oestradiol and norethisterone had similar whole-blood platelet aggregation rates and maximum platelet aggregation responses to higher doses of platelet agonists when compared with those on placebo. The endogenous platelet nitric oxide system did not appear to affect aggregation in either group.

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