scholarly journals Analysis of the Influence of Hormone Replacement Therapy on Osteocalcin Gene Expression in Postmenopausal Women

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Mansur Rahnama ◽  
Izabela Jastrzębska-Jamrogiewicz ◽  
Rafał Jamrogiewicz ◽  
Grzegorz Trybek
Keyword(s):  
Gene Expression ◽  
Hormone Replacement Therapy ◽  
Replacement Therapy ◽  
Bone Metabolism ◽  
Postmenopausal Women ◽  
Bone Mineralization ◽  
Hormone Replacement ◽  
Peripheral Blood Lymphocytes ◽  
Local Bone ◽  
Osteocalcin Gene

Background.Osteocalcin (OC) contributes to the process of bone mineralization. Present study was designed to investigate the changes in OC gene expression of postmenopausal women treated with hormone replacement therapy (HRT). Study was also designed to evaluate OC gene expression in cells which are not part of connective tissue.Material and Methods.Research was carried out on 30 postmenopausal women not treated and 30 treated with HRT. Examination of OC gene expression was conducted on peripheral blood lymphocytes (PBL) and buccal epithelial lining (BEL). Densitometry was conducted on femur and mandible.Results.Tests revealed OC gene expression in BEL and PBL. BMD was higher in groups treated with HRT. Assessment of correlation between the OC gene expression in BEL and BMD of mandible revealed significant positive relation.Conclusions.OC gene expression can be stated BEL and PBL. Analysis of correlation between OC gene expression in oral cavity and mandible BMD showed significant correlation between local OC expression and local bone metabolism. The relation between OC gene expression and bone metabolism is complex and further research is needed to clear all of the uncertainties.

scholarly journals Hormone replacement therapy dependent changes in breast cancer-related gene expression in breast tissue of healthy postmenopausal women

2011 ◽  
Vol 5 (6) ◽  
pp. 504-516 ◽  
Author(s):  
Anieta M. Sieuwerts ◽  
Giuseppina De Napoli ◽  
Anne van Galen ◽  
Helenius J. Kloosterboer ◽  
Vanja de Weerd ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Gene Expression ◽  
Hormone Replacement Therapy ◽  
Replacement Therapy ◽  
Postmenopausal Women ◽  
Breast Tissue ◽  
Hormone Replacement ◽  
Related Gene ◽  
Cancer Related Gene

scholarly journals Influence of hormone replacement therapy on eccentric exercise induced myogenic gene expression in postmenopausal women

2009 ◽  
Vol 107 (5) ◽  
pp. 1381-1388 ◽  
Author(s):  
Christina M. Dieli-Conwright ◽  
Tanya M. Spektor ◽  
Judd C. Rice ◽  
Fred R. Sattler ◽  
E. Todd Schroeder
Keyword(s):  
Gene Expression ◽  
Hormone Replacement Therapy ◽  
Mrna Expression ◽  
Replacement Therapy ◽  
Postmenopausal Women ◽  
Eccentric Exercise ◽  
Hormone Replacement ◽  
Control Group ◽  
Regulatory Factor ◽  
Myogenic Regulatory Factor

Hormone replacement therapy (HRT) is used in postmenopausal women to relieve symptoms of menopause and prevent osteoporosis. We sought to evaluate changes in mRNA expression of key myogenic factors in postmenopausal women taking and not taking HRT following a high-intensity eccentric resistance exercise. Fourteen postmenopausal women were studied and included 6 control women not using HRT (59 ± 4 years, 63 ± 17 kg) and 8 women using traditional HRT (59 ± 4 yr, 89 ± 24 kg). Both groups performed 10 sets of 10 maximal eccentric repetitions of single-leg extension on a Cybex dynamometer at 60°/s. Muscle biopsies of the vastus lateralis were obtained from the exercised leg at baseline and 4 h after the exercise bout. Gene expression was determined using RT-PCR for follistatin, forkhead box 3A (FOXO3A), muscle atrophy F-box (MAFbx), muscle ring finger-1 (MuRF-1), myogenic differentiation factor (MyoD), myogenin, myostatin, myogenic factor 5 (Myf5), and muscle regulatory factor 4 (MRF4). At rest, the HRT group expressed higher levels of MyoD, myogenin, Myf5, MRF4, and follistatin ( P < 0.05). In response to eccentric exercise, follistatin, MyoD, myogenin, Myf5, and MRF4 were significantly increased ( P ≤ 0.05) and FOXO3A, MAFbx, MuRF-1, and myostatin were significantly decreased in the control and HRT groups ( P ≤ 0.05). Significantly greater changes in mRNA expression of follistatin, FOXO3A, MAFbx, MuRF-1, MyoD, myogenin, myostatin, Myf5, and MRF4 (p≤0.05) occurred in the HRT group than in the control group after exercise. These data suggest that postmenopausal women using HRT express higher myogenic regulatory factor gene expression, which may reflect an attempt to preserve muscle mass. Furthermore, postmenopausal women using HRT experienced a greater myogenic response to maximal eccentric exercise.

scholarly journals The effect of hormone replacement therapy on the expression of the alkaline phosphatase gene (ALPL) within the mucosal epithelium of the cheek and in peripheral blood lymphocytes

2014 ◽  
Vol 27 (2) ◽  
pp. 92-96 ◽  
Author(s):  
Mansur Rahnama ◽  
Michal Lobacz ◽  
Anna Szyszkowska ◽  
Grzegorz Trybek ◽  
Maryla Kozicka-Czupkallo
Keyword(s):  
Alkaline Phosphatase ◽  
Hormone Replacement Therapy ◽  
Bone Formation ◽  
Replacement Therapy ◽  
Peripheral Blood ◽  
Bone Mineralization ◽  
Hormone Replacement ◽  
Peripheral Blood Lymphocytes ◽  
Mucosal Epithelium ◽  
Osteoblast Activity

Abstract In adult life, proper bone metabolism requires efficient regulation of bone formation and resorption processes. Bone turnover markers allow for assessing the rate of bone formation and resorption processes. In menopausal period, female patients experience gradual reduction in blood estradiol levels. The deficit of estrogens leads to enhanced osteoclastogenesis and bone resorption. Alkaline phosphatase (ALP) is a membranebound enzyme that stimulates the osteoblast activity and bone mineralization. It is synthesized by osteoblasts and incorporated into the newly formed bone tissue. The produced enzyme stimulates the osteoblast activity and bone mineralization. The goal of this study is to determine the effect of hormone replacement therapy in postmenopausal women on the expression of alkaline phosphatase gene (ALPL) within the mucosal epithelium of the cheek and in peripheral blood lymphocytes. The studies show that hormone replacement therapy has no significant effect on the increase in ALPL expression within the mucosal epithelium of the cheek. Only in women having undergone ovarectomy (OV), the epithelial ALPL expression level was higher than in the remaining groups.

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