Gene expression profiling in human whole blood samples after controlled testosterone application and exercise

2011 ◽  
Vol 3 (10) ◽  
pp. 652-660 ◽  
Author(s):  
Martin Schönfelder ◽  
Hande Hofmann ◽  
Patricia Anielski ◽  
Detlef Thieme ◽  
Renate Oberhoffer ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Expression Profiling ◽  
Whole Blood ◽  
Expression Profiling ◽  
Blood Samples ◽  
Human Whole Blood ◽  
Whole Blood Samples

scholarly journals Gene expression profiling of human whole blood samples with the Illumina WG-DASL assay

BMC Genomics ◽  
2011 ◽  
Vol 12 (1) ◽  
Author(s):  
Mary E Winn ◽  
Marian Shaw ◽  
Craig April ◽  
Brandy Klotzle ◽  
Jian-Bing Fan ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Expression Profiling ◽  
Whole Blood ◽  
Expression Profiling ◽  
Blood Samples ◽  
Human Whole Blood ◽  
Whole Blood Samples

scholarly journals Effects of Storage, RNA Extraction, Genechip Type, and Donor Sex on Gene Expression Profiling of Human Whole Blood

2007 ◽  
Vol 53 (6) ◽  
pp. 1038-1045 ◽  
Author(s):  
Sung Jae Kim ◽  
David J Dix ◽  
Kary E Thompson ◽  
Rachel N Murrell ◽  
Judith E Schmid ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Expression Profiling ◽  
Whole Blood ◽  
Expression Profiling ◽  
Expression Profiles ◽  
Rna Extraction ◽  
Extraction Technique ◽  
Interindividual Differences ◽  
Human Whole Blood ◽  
Rna Quality

Abstract Background: Gene expression profiling of whole blood may be useful for monitoring toxicological exposure and for diagnosis and monitoring of various diseases. Several methods are available that can be used to transport, store, and extract RNA from whole blood, but it is not clear which procedures alter results. In addition, characterization of interindividual and sex-based variation in gene expression is needed to understand sources and extent of variability. Methods: Whole blood was obtained from adult male and female volunteers (n = 42) and stored at various temperatures for various lengths of time. RNA was isolated and RNA quality analyzed. Affymetrix GeneChips (n = 23) were used to characterize gene expression profiles (GEPs) and to determine the effects on GEP of storage conditions, extraction techniques, types of GeneChip, or donor sex. Hierarchical clustering and principal component analysis were used to assess interindividual differences. Regression analysis was used to assess the relative impact of the studied variables. Results: Storage of blood samples for >1 week at 4 °C diminished subsequent RNA quality. Interindividual GEP differences were seen, but larger effects were observed related to RNA extraction technique, GeneChip, and donor sex. The relative importance of the variables was as follows: storage < genechip < extraction technique < donor sex. Conclusion: Sample storage and extraction methods and interindividual differences, particularly donor sex, affect GEP of human whole blood.

Gene expression profiling of the effects of intravenous immunoglobulin in human whole blood

2006 ◽  
Vol 43 (7) ◽  
pp. 939-949 ◽  
Author(s):  
Marco Ghielmetti ◽  
Michel Bellis ◽  
Martin O. Spycher ◽  
Sylvia Miescher ◽  
Guy Vergères
Keyword(s):  
Gene Expression ◽  
Gene Expression Profiling ◽  
Intravenous Immunoglobulin ◽  
Whole Blood ◽  
Expression Profiling ◽  
Human Whole Blood

scholarly journals Isolation and characterization of circulating pro-vascular progenitor cell subsets from human whole blood samples

2021 ◽  
Vol 2 (1) ◽  
pp. 100311
Author(s):  
Daniella C. Terenzi ◽  
Ehab Bakbak ◽  
Justin Z. Trac ◽  
Mohammad Al-Omran ◽  
Adrian Quan ◽  
...  
Keyword(s):  
Whole Blood ◽  
Progenitor Cell ◽  
Blood Samples ◽  
Human Whole Blood ◽  
Isolation And Characterization ◽  
Whole Blood Samples

The distribution of BrKα/RbKα peak intensity ratio in human whole blood samples

1994 ◽  
Vol 42 (3) ◽  
pp. 231-241 ◽  
Author(s):  
C. Shenberg ◽  
S. Spiegel ◽  
S. Chaitchik ◽  
P. Jordan ◽  
M. Kitzis ◽  
...  
Keyword(s):  
Whole Blood ◽  
Intensity Ratio ◽  
Peak Intensity Ratio ◽  
Blood Samples ◽  
Human Whole Blood ◽  
Peak Intensity ◽  
Whole Blood Samples
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