scholarly journals Effect of small interference RNA (siRNA) for ADAMTS5 on intervertebral disc degeneration in the rabbit anular needle-puncture model

2009 ◽  
Vol 11 (6) ◽  
pp. R166 ◽  
Author(s):  
Shoji Seki ◽  
Yumiko Asanuma-Abe ◽  
Koichi Masuda ◽  
Yoshiharu Kawaguchi ◽  
Kunihiro Asanuma ◽  
...  
Keyword(s):  
Intervertebral Disc ◽  
Disc Degeneration ◽  
Intervertebral Disc Degeneration ◽  
Needle Puncture ◽  
Small Interference Rna ◽  
Puncture Model ◽  
Interference Rna

P89. Oral administration of a prodrug of esculetin derivative inhibits intervertebral disc degeneration in the rabbit needle puncture model

2004 ◽  
Vol 4 (5) ◽  
pp. S102-S103
Author(s):  
Masahiko Okuma ◽  
Howard An ◽  
Koichi Nakagawa ◽  
Koji Akeda ◽  
Carol Muehleman ◽  
...  
Keyword(s):  
Intervertebral Disc ◽  
Oral Administration ◽  
Disc Degeneration ◽  
Intervertebral Disc Degeneration ◽  
Needle Puncture ◽  
Puncture Model

scholarly journals Intervertebral Disc Degeneration Induced by Needle Puncture and Ovariectomy: A Rat Coccygeal Model

2021 ◽  
Vol 2021 ◽  
pp. 1-7
Author(s):  
Tao Tian ◽  
Haidong Wang ◽  
Zhaohui Li ◽  
Sidong Yang ◽  
Wenyuan Ding
Keyword(s):  
Animal Model ◽  
Intervertebral Disc ◽  
Disc Degeneration ◽  
Intervertebral Disc Degeneration ◽  
Corn Oil ◽  
Female Rats ◽  
Needle Puncture ◽  
Time Points ◽  
17Β Estradiol ◽  
Safranin O

Objectives. To establish a novel animal model of intervertebral disc degeneration (IVDD) in rats and to investigate the effect of 17β-estradiol (E2) intervention in this model. Methods. This study was divided into two parts: animal model (four groups: Sham, ovariectomy (OVX), Puncture, and OVX+Puncture; three-time points: 4, 8, and 12 weeks; three female rats/group/time point) and drug intervention (Sham, OVX+Puncture+corn oil, and OVX+Puncture+E2; three female rats/group). The rats were analyzed by micromagnetic resonance imaging (MRI), hematoxylin and eosin (HE) staining, and safranin-O staining. Results. MRI and histological scores significantly differed among the four groups at the three-time points (all P < 0.05 ). IVDD progressed with time in the OVX, Puncture, and OVX+Puncture groups (all P < 0.05 ). The changes were the most obvious in the OVX+Puncture group. In the E2 intervention part, the Veh group had the worst MRI signals and histological scores ( P < 0.05 ). The MRI scores in the E2 group were less obvious compared to the Sham group ( P > 0.05 ). Also, the histological scores were significantly different between the Sham and E2 groups ( P < 0.05 ). Conclusions. The combination of ovariectomy and needle puncture can synergically induce IVDD in rat coccygeal discs. Estrogen treatment can effectively ameliorate IVDD progression in the newly established IVDD models.

scholarly journals A percutaneous, minimally invasive annulus fibrosus needle puncture model of intervertebral disc degeneration in rabbits

2018 ◽  
Vol 26 (3) ◽  
pp. 230949901879271
Author(s):  
T David Luo ◽  
Alejandro Marquez-Lara ◽  
Zachary K Zabarsky ◽  
Jeremy B Vines ◽  
Katie C Mowry ◽  
...  
Keyword(s):  
Relaxation Time ◽  
Minimally Invasive ◽  
Intervertebral Disc ◽  
Disc Degeneration ◽  
Annulus Fibrosus ◽  
Intervertebral Disc Degeneration ◽  
Disc Height ◽  
Needle Puncture ◽  
T2 Relaxation Time ◽  
T2 Relaxation

Purpose: Various animal models have been proposed to mimic the pathophysiologic process of intervertebral disc degeneration, a leading cause of back pain. The purpose of this study is to describe a minimally invasive technique via percutaneous needle puncture of the annulus fibrosus in New Zealand white rabbits. Methods: Under fluoroscopic guidance, an 18-gauge spinal needle was inserted 2 cm lateral to the midline spinous process. The needle was slowly advanced at approximately 45° angle until it was adjacent to the L5/L6 disc space. Lateral and anteroposterior views were used to verify correct needle position before advancing into the nucleus pulposus. The rabbits underwent weekly X-rays for 4 weeks to assess disc height index. MRI T2 relaxation was evaluated at week four to assess morphological changes. Discs were histologically graded on a 12-point scale to assess degeneration and compared to discs obtained from uninjured rabbits. Results: There were no complications associated with the percutaneous needle puncture procedure. All animals survived the duration of the experiment. Four weeks after injury, the disc height had progressively narrowed to approximately 50% of baseline. MRI assessment at the 4-week time point demonstrated a mean T2 relaxation time at the L5/L6 level that was 20.9% of the T2 relaxation time at the uninjured L4/L5 disc level ( p < 0.001). Histological analysis demonstrated lamellar disorganization of the annulus and decreased cellularity and proteoglycan content within the injured nucleus compared to uninjured control discs. Conclusion: The present study demonstrated a reliable technique of inducing an annular tear via a percutaneous needle puncture. Compared to open surgical approaches, the percutaneous model produces similar progressive disc degeneration while minimizing harm to the animal subjects. Clinical Relevance: The present study establishes a technique for the introduction of novel therapeutic agents to treat disc degeneration that may translate to future clinical trials.

Time course investigation of intervertebral disc degeneration in a rat-tail puncture model

Life Sciences ◽  
2016 ◽  
Vol 156 ◽  
pp. 15-20 ◽  
Author(s):  
Chia-Hsian Chen ◽  
Chang-Jung Chiang ◽  
Lien-Chen Wu ◽  
Chih-Hong Yang ◽  
Yi-Jie Kuo ◽  
...  
Keyword(s):  
Intervertebral Disc ◽  
Disc Degeneration ◽  
Intervertebral Disc Degeneration ◽  
Time Course ◽  
Puncture Model ◽  
Rat Tail

scholarly journals Danshen Attenuates Intervertebral Disc Degeneration via Antioxidation in SD Rats

2020 ◽  
Vol 2020 ◽  
pp. 1-12
Author(s):  
Rongqing Qin ◽  
Shouqian Dai ◽  
Xing Zhang ◽  
Hongpeng Liu ◽  
Bing Zhou ◽  
...  
Keyword(s):  
Intervertebral Disc ◽  
Disc Degeneration ◽  
Intervertebral Disc Degeneration ◽  
Male Rats ◽  
Inflammatory Factors ◽  
Control Group ◽  
Needle Puncture ◽  
Sd Rats ◽  
Associated Proteins ◽  
Safranin O

Objective. To investigate the effects of Danshen on the imaging and histological parameters, expression levels of ECM-associated proteins and inflammatory factors, and antioxidative activity in the degenerated intervertebral disc (IVD) of SD rats. Methods. Sixty male rats were randomly divided into three groups (control, IDD, and Danshen IDD). Percutaneous needle puncture in Co8-9 intervertebral disc was conducted in all rats of the IDD and Danshen IDD groups to induce intervertebral disc degeneration (IDD). After operation, animals of the Danshen IDD group were administrated with Danshen granules (3 g/kg body weight ) by gavage once a day. Four weeks later, the coccygeal vertebrae were harvested and used for imaging (disc height and MR signal), histological, immunohistochemical, and biochemical [water content, glycosaminoglycans (GAG), superoxide dismutase (SOD2), glutathione (GSH), and malondialdehyde (MDA)] analyses. Results. The puncture induced significant decreased IVD space and MR T2 signal at both 2 and 4 weeks, which were attenuated by Danshen treatment. The disc degeneration in the IDD group (HE and Safranin O-Fast Green histological staining was markedly more serious compared with that in the control group. Four weeks of Danshen treatment significantly alleviated this degeneration compared with the IDD group. Needle puncture resulted in the upregulation of IL-1β and TNF-α, MMP-3, and downregulation of COL2 and aggrecan in the IDD group. However, this change was significantly weakened by Danshen treatment. Significantly lower water and GAG content, as well as the SOD2 and GSH levels, in the IDD group were found compared with those in the control group. However, the above parameters of the Danshen IDD group were significantly higher than those of the IDD group. Danshen treatment significantly decreased the content of MDA which was increased by needle puncture in the IDD group. Conclusion. Danshen can attenuate intervertebral disc degeneration in SD rats by suppressing the oxidation reaction.

scholarly journals Needle puncture injury causes acute and long-term mechanical deficiency in a mouse model of intervertebral disc degeneration

2013 ◽  
Vol 31 (8) ◽  
pp. 1276-1282 ◽  
Author(s):  
John T. Martin ◽  
Deborah J. Gorth ◽  
Elizabeth E. Beattie ◽  
Brian D. Harfe ◽  
Lachlan J. Smith ◽  
...  
Keyword(s):  
Mouse Model ◽  
Intervertebral Disc ◽  
Disc Degeneration ◽  
Intervertebral Disc Degeneration ◽  
Needle Puncture ◽  
Injury Causes
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