scholarly journals Needle puncture injury causes acute and long-term mechanical deficiency in a mouse model of intervertebral disc degeneration

2013 ◽  
Vol 31 (8) ◽  
pp. 1276-1282 ◽  
Author(s):  
John T. Martin ◽  
Deborah J. Gorth ◽  
Elizabeth E. Beattie ◽  
Brian D. Harfe ◽  
Lachlan J. Smith ◽  
...  
Keyword(s):  
Mouse Model ◽  
Intervertebral Disc ◽  
Disc Degeneration ◽  
Intervertebral Disc Degeneration ◽  
Needle Puncture ◽  
Injury Causes

scholarly journals Ligustrazine Prevents Intervertebral Disc Degeneration via Suppression of Aberrant TGFβ Activation in Nucleus Pulposus Cells

2019 ◽  
Vol 2019 ◽  
pp. 1-9
Author(s):  
Shufen Liu ◽  
Yuhao Cheng ◽  
Yuqi Tan ◽  
Jingcheng Dong ◽  
Qin Bian
Keyword(s):  
Growth Factor ◽  
Mouse Model ◽  
Intervertebral Disc ◽  
Nucleus Pulposus ◽  
Disc Degeneration ◽  
Intervertebral Disc Degeneration ◽  
Ex Vivo ◽  
Tissue Growth ◽  
Nucleus Pulposus Cells

Objectives. Aberrant transforming growth factor β (TGFβ) activation is detrimental to both nucleus pulposus (NP) cells and cartilage endplates (CEPs), which can lead to intervertebral disc degeneration (IDD). Ligustrazine (LIG) reduces the expression of inflammatory factors and TGFβ1 in hypertrophic CEP to prevent IDD. In this study, we investigate the effects of LIG on NP cells and the TGFβ signaling. Design. LIG was injected to the lumbar spinal instability (LSI) mouse model. The effect of LIG was evaluated by intervertebral disc (IVD) score in the LSI mouse model. The expression of activated TGFβ was examined using immunostaining with pSmad2/3 antibody. The upright posture (UP) rat model was also treated and evaluated in the same manner to assess the effect of LIG. In ex vivo study, IVDs from four-week old mice were isolated and treated with 10−5, 10−6, and 10−7 M of LIG. We used western blot to detect activated TGFβ expression. TGFβ-treated human nucleus pulposus cells (HNPCs) were cotreated with optimized dose of LIG in vitro. Immunofluorescence staining was performed to determine pSmad2/3, connective tissue growth factor (CCN2), and aggrecan (ACAN) expression levels. Results. IVD score and the percentage of pSmad2/3+ NP cells were low in LIG-treated LSI mice in comparison with LSI mice, but close to the levels in the Sham group. Similarly, LIG reduced the overexpression of TGFβ1 in NP cells. The inhibitory effect of LIG was dose dependent. A dose of 10−5 M LIG not only strongly attenuated Smad2/3 phosphorylation in TGFβ-treated IVD ex vivo but also suppressed pSmad2/3, CCN2, and ACAN expression in TGFβ-treated NP cells in vitro. Conclusions. LIG prevents IDD via suppression of TGFβ overactivation in NP cells.

Mechanics and Biology Interact in Intervertebral Disc Degeneration: A Novel Composite Mouse Model

2020 ◽  
Vol 106 (4) ◽  
pp. 401-414 ◽  
Author(s):  
Zhi-feng Xiao ◽  
Guo-yi Su ◽  
Yu Hou ◽  
Shu-dong Chen ◽  
Bing-de Zhao ◽  
...  
Keyword(s):  
Mouse Model ◽  
Intervertebral Disc ◽  
Disc Degeneration ◽  
Intervertebral Disc Degeneration

scholarly journals Long-term treatment with senolytic drugs Dasatinib and Quercetin ameliorates age-dependent intervertebral disc degeneration in mice

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Emanuel J. Novais ◽  
Victoria A. Tran ◽  
Shira N. Johnston ◽  
Kayla R. Darris ◽  
Alex J. Roupas ◽  
...  
Keyword(s):  
Intervertebral Disc ◽  
Disc Degeneration ◽  
Intervertebral Disc Degeneration ◽  
Chronic Back Pain ◽  
The Elderly ◽  
Term Treatment ◽  
Specific Effects ◽  
Long Term Treatment ◽  
Age Dependent

AbstractIntervertebral disc degeneration is highly prevalent within the elderly population and is a leading cause of chronic back pain and disability. Due to the link between disc degeneration and senescence, we explored the ability of the Dasatinib and Quercetin drug combination (D + Q) to prevent an age-dependent progression of disc degeneration in mice. We treated C57BL/6 mice beginning at 6, 14, and 18 months of age, and analyzed them at 23 months of age. Interestingly, 6- and 14-month D + Q cohorts show lower incidences of degeneration, and the treatment results in a significant decrease in senescence markers p16INK4a, p19ARF, and SASP molecules IL-6 and MMP13. Treatment also preserves cell viability, phenotype, and matrix content. Although transcriptomic analysis shows disc compartment-specific effects of the treatment, cell death and cytokine response pathways are commonly modulated across tissue types. Results suggest that senolytics may provide an attractive strategy to mitigating age-dependent disc degeneration.

scholarly journals Effect of small interference RNA (siRNA) for ADAMTS5 on intervertebral disc degeneration in the rabbit anular needle-puncture model

2009 ◽  
Vol 11 (6) ◽  
pp. R166 ◽  
Author(s):  
Shoji Seki ◽  
Yumiko Asanuma-Abe ◽  
Koichi Masuda ◽  
Yoshiharu Kawaguchi ◽  
Kunihiro Asanuma ◽  
...  
Keyword(s):  
Intervertebral Disc ◽  
Disc Degeneration ◽  
Intervertebral Disc Degeneration ◽  
Needle Puncture ◽  
Small Interference Rna ◽  
Puncture Model ◽  
Interference Rna

scholarly journals Intervertebral Disc Degeneration Induced by Needle Puncture and Ovariectomy: A Rat Coccygeal Model

2021 ◽  
Vol 2021 ◽  
pp. 1-7
Author(s):  
Tao Tian ◽  
Haidong Wang ◽  
Zhaohui Li ◽  
Sidong Yang ◽  
Wenyuan Ding
Keyword(s):  
Animal Model ◽  
Intervertebral Disc ◽  
Disc Degeneration ◽  
Intervertebral Disc Degeneration ◽  
Corn Oil ◽  
Female Rats ◽  
Needle Puncture ◽  
Time Points ◽  
17Β Estradiol ◽  
Safranin O

Objectives. To establish a novel animal model of intervertebral disc degeneration (IVDD) in rats and to investigate the effect of 17β-estradiol (E2) intervention in this model. Methods. This study was divided into two parts: animal model (four groups: Sham, ovariectomy (OVX), Puncture, and OVX+Puncture; three-time points: 4, 8, and 12 weeks; three female rats/group/time point) and drug intervention (Sham, OVX+Puncture+corn oil, and OVX+Puncture+E2; three female rats/group). The rats were analyzed by micromagnetic resonance imaging (MRI), hematoxylin and eosin (HE) staining, and safranin-O staining. Results. MRI and histological scores significantly differed among the four groups at the three-time points (all P < 0.05 ). IVDD progressed with time in the OVX, Puncture, and OVX+Puncture groups (all P < 0.05 ). The changes were the most obvious in the OVX+Puncture group. In the E2 intervention part, the Veh group had the worst MRI signals and histological scores ( P < 0.05 ). The MRI scores in the E2 group were less obvious compared to the Sham group ( P > 0.05 ). Also, the histological scores were significantly different between the Sham and E2 groups ( P < 0.05 ). Conclusions. The combination of ovariectomy and needle puncture can synergically induce IVDD in rat coccygeal discs. Estrogen treatment can effectively ameliorate IVDD progression in the newly established IVDD models.

scholarly journals A novel mouse model of intervertebral disc degeneration shows altered cell fate and matrix homeostasis

2018 ◽  
Vol 70 ◽  
pp. 102-122 ◽  
Author(s):  
Hyowon Choi ◽  
Steven Tessier ◽  
Elizabeth S. Silagi ◽  
Rutvin Kyada ◽  
Farzad Yousefi ◽  
...  
Keyword(s):  
Mouse Model ◽  
Intervertebral Disc ◽  
Cell Fate ◽  
Disc Degeneration ◽  
Intervertebral Disc Degeneration ◽  
Altered Cell
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