scholarly journals HISTOCHEMICAL STUDY OF A FLUORIDE RESISTANT ACID PHOSPHATASE REACTION IN THE MOUSE DUODENUM

1957 ◽  
Vol 5 (2) ◽  
pp. 135-139 ◽  
Author(s):  
ROBERT C. BURT ◽  
BARBARA R. MEREDITH ◽  
ROBERT C. GRAUER
Keyword(s):  
Acid Phosphatase ◽  
Histochemical Study ◽  
Acid Phosphatase Reaction

scholarly journals Arrested apoptosis without nuclear fragmentation produced by efferent duct ligation in round spermatids and multinucleated giant cells of rat testis

Reproduction ◽  
2003 ◽  
pp. 879-887 ◽  
Author(s):  
E Anton
Keyword(s):  
Acid Phosphatase ◽  
Nuclear Membrane ◽  
Sertoli Cells ◽  
Giant Cells ◽  
Differential Staining ◽  
Multinucleated Giant Cells ◽  
Nuclear Fragmentation ◽  
Efferent Duct ◽  
Duct Ligation ◽  
Acid Phosphatase Reaction

The apoptotic process evoked by efferent duct ligation in the testes of adult rats was followed for 10 days by differential staining for haematoxylin-eosin, periodic acid-Schiff and a modified trichrome technique in optical microscopy and by ultrastructural localization of acid phosphatase. Round spermatids showed the first effects of efferent duct ligation. At day 3 after ligation, annular clumps of chromatin with typical apoptotic characteristics appeared against the nuclear membrane of these cells. Afterwards, membranous structures and a wide separation between the two layers of the nuclear membrane were observed but nuclear fragmentation did not occur and apoptotic granules were not seen. Cytoplasmic components were also altered, and severely damaged organoids and empty vacuoles lacking acid phosphatase reaction were frequently seen. On day 2 after efferent duct ligation, multinucleated giant cells appeared, which displayed similar characteristics as spermatids and showed no acid phosphatase reaction. Although abnormal spermatids and multinucleated giant cells were surrounded by the cytoplasm of Sertoli cells, neither lysosomal acid phosphatase nor phagocytic activity was detected. It is concluded that efferent duct ligation specifically affects round immature spermatids eliciting a partial nuclear apoptotic response that is not accompanied by autophagic or heterophagic activity and without lysosomal participation in Sertoli cells.

Quantitative X-ray microanalysis of spleen lysosomes after acid phosphatase reaction

1982 ◽  
Vol 75 (4) ◽  
pp. 485-491
Author(s):  
G. M. Roomans ◽  
R. Wróblewski
Keyword(s):  
Acid Phosphatase ◽  
X Ray ◽  
Acid Phosphatase Reaction

Histochemical study of acid phosphatase activity in cerebral tumors

1965 ◽  
Vol 5 (1) ◽  
pp. 16-25 ◽  
Author(s):  
D. Schiffer ◽  
A. Fabiani ◽  
G. F. Monticone ◽  
G. Gabella
Keyword(s):  
Acid Phosphatase ◽  
Phosphatase Activity ◽  
Acid Phosphatase Activity ◽  
Histochemical Study ◽  
Cerebral Tumors

scholarly journals ACID PHOSPHATASE ACTIVITY IN INDIVIDUAL NEURONS DURING CHROMATOLYSIS: A QUANTITATIVE HISTOCHEMICAL STUDY

1970 ◽  
Vol 18 (11) ◽  
pp. 828-833 ◽  
Author(s):  
HILDE E. HIRSCH ◽  
THEODORE OBENCHAIN
Keyword(s):  
Spinal Cord ◽  
Acid Phosphatase ◽  
Phosphatase Activity ◽  
Acid Phosphatase Activity ◽  
Histochemical Study ◽  
Galactosidase Activity ◽  
Fluorogenic Substrates ◽  
Striking Increase ◽  
Staining Methods ◽  
Glucose 6 Phosphate Dehydrogenase

Two fluorogenic substrates, α-naphthyl phosphate and 4-methylumbelliferyl phosphate, were used to measure acid phosphatase activities in individual anterior horn neurons of the monkey spinal cord after section of the sciatic nerve. Although many studies utilizing staining methods have reported a striking increase in acid phosphatase activity during chromatolysis, no significant differences were observed here between the neurons of the operated and unoperated side, despite widespread chromatolysis. β-galactosidase activity was also unchanged, but the marked elevation of glucose 6-phosphate dehydrogenase during the reparative phase was confirmed.

A histochemical study of starch, lipids, and certain enzymes in senescing rose stems

1977 ◽  
Vol 55 (6) ◽  
pp. 617-624 ◽  
Author(s):  
J. M. Molnar ◽  
E. V. Parups
Keyword(s):  
Acid Phosphatase ◽  
Cytochrome Oxidase ◽  
Lipid Content ◽  
Histochemical Study ◽  
Succinic Dehydrogenase ◽  
Sugar Solution ◽  
Lipid Peroxidase ◽  
High Level ◽  
The Rose ◽  
Cut Rose

The starch, lipid, cytochrome oxidase (EC 1.9.3.1), succinic dehydrogenase (EC 1.3.99.1), peroxidase (EC 1.11.1.7), and acid phosphatase (EC 3.1.3.2) levels were determined periodically by histochemical methods in transverse sections of cut stem of the rose, Rosa hybrida L. cv. Forever Yours, kept in water or in an aqueous preservative solution containing 4% sucrose, 100 ppm sodium isoascorbate, and 100 ppm 8-hydroxyquinoline sulfate. Senescence of the cutrose stem, including leaves and flowers, was delayed by use of the sugar solution. The levels of cytochrome oxidase and succinic dehydrogenase were not significantly affected by either of the treatments. Starch, lipid, peroxidase, and acid phosphatase levels decreased in the tissues of rapidly senescing stems kept in water. In tissues where senescence was retarded by use of sugar solutions the lipid content and peroxidase were maintained at a relatively high level; starch, and acid phosphatase levels steadily increased. It is suggested that in cut rose stems, the onset or retardation of senescence is not related to the activities of acid phosphatase or peroxidase.

ACID-PHOSPHATASE REACTION IN ACUTE LYMPHOBLASTIC LEUKÆMIA

The Lancet ◽  
1978 ◽  
Vol 311 (8067) ◽  
pp. 749-751 ◽  
Author(s):  
D. Catovsky ◽  
M.F. Greaves ◽  
C. Pain ◽  
M. Cherchi ◽  
G. Janossy ◽  
...  
Keyword(s):  
Acid Phosphatase ◽  
Acute Lymphoblastic Leukaemia ◽  
Lymphoblastic Leukaemia ◽  
Acid Phosphatase Reaction

scholarly journals Structural responses of amoebae to the injection of heterologous cytoplasm

1980 ◽  
Vol 45 (1) ◽  
pp. 1-14
Author(s):  
C.J. Flickinger ◽  
G.A. Read ◽  
E.M. Kabana
Keyword(s):  
Acid Phosphatase ◽  
Survival Rate ◽  
Light Microscopy ◽  
Lethal Effect ◽  
Cytoplasmic Vacuoles ◽  
Donor Cells ◽  
Structural Responses ◽  
Cellular Components ◽  
Acid Phosphatase Reaction ◽  
General Appearance

Responses to the introduction of heterologous cytoplasm and the fate of foreign organelles were investigated in amoebae. Heterologous cytoplasm was transferred by microinjection from Pelomyxa carolinensis to Amoeba discoides. In control experiments, homologous cytoplasm was transferred from one A. discoides to another. Recipient cells were observed by light microscopy, and samples were prepared for ultrastructural study at intervals between 15 min nad 3 days after operation. Recipients of heterologous cytoplasm showed two main responses. First, about 40% recipients expelled small amounts of cytoplasm by a blebbing process within 30 min after injection. Second, organelles were segregated and broken down in membrane-bounded cytoplasmic vacuoles between 6 h and 2 days after operation. Acid phosphatase reaction product was observed in these vacuoles along with altered organelles. Use of electron-dense thoria particles to mark donor cells demonstrated the presence of injected cytoplasm in the vacuoles. In contrast, when amoebae were injected with homologous cytoplasm, none was expelled and vacuoles containing degenerating organelles were uncommon. The survival rate and general appearance of recipients of heterologous cytoplasm were much poorer than those of homologous recipients, and most of the former died by I week after operation. It is postulated that amoeba are capable of recognizing heterologous organelles introduced into the cytoplasm and that they respond by expulsion and/or destruction of the foreign cellular components. The previously described lethal effect of heterologous cytoplasm was confirmed.

scholarly journals A HISTOCHEMICAL STUDY OF PHAGOCYTIC AND ENZYMATIC FUNCTIONS OF RABBIT MONONUCLEAR AND POLYMORPHONUCLEAR EXUDATE CELLS AND ALVEOLAR MACROPHAGES

1963 ◽  
Vol 17 (3) ◽  
pp. 465-486 ◽  
Author(s):  
Arthur M. Dannenberg ◽  
Marvin S. Burstone ◽  
Paul C. Walter ◽  
June W. Kinsley
Keyword(s):  
Alkaline Phosphatase ◽  
Acid Phosphatase ◽  
Alveolar Macrophages ◽  
Histochemical Study ◽  
Succinic Dehydrogenase ◽  
Enzymatic Activities ◽  
Dust Particles ◽  
Cell Type ◽  
Microbial Agents

The cytochrome oxidase (CO), aminopeptidase (AMP), succinic dehydrogenase (SD), acid phosphatase, esterase, and alkaline phosphatase of rabbit mononuclear (MN) and polymorphonuclear (PMN) peritoneal exudate cells and pulmonary alveolar macrophages (AM) - air dried on Mylar strips - were characterized by histochemical techniques with respect to stability, activators, inhibitors, and pH optima. A granule count method was established for the quantitation of these enzymes. For the acid phosphatase of MN, in which the most precise results were obtained, time, pH, substrate, and inhibitor curves resembled those commonly obtained biochemically. Five of these enzymes were usually more active in AM than MN, whereas the sixth, alkaline phosphatase, was not present in either cell type. AM also tended to consume more oxygen than MN and to divide more frequently. Since the most active cells in the population would be first involved in the host's defense against microbial agents, a comparison was made of the 10 per cent of the AM and MN with the highest enzymatic activities. No differences were found in the granule counts that were not reflected by the means. However, within a given AM population, cells containing ingested dust particles seemed to have higher enzymatic activities than those without particles. MN had greater acid phosphatase and SD activities than PMN and consumed more oxygen, but the CO, AMP, and esterase activites of both types of cells were of similar magnitude. PMN showed high alkaline phosphatase activity; MN showed none. A survey of the histochemical literature indicates that a positive correlation between the enzymatic and phagocytic activities of both MN and PMN exists in vivo.

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