Histochemical study of acid phosphatase activity in cerebral tumors

1965 ◽  
Vol 5 (1) ◽  
pp. 16-25 ◽  
Author(s):  
D. Schiffer ◽  
A. Fabiani ◽  
G. F. Monticone ◽  
G. Gabella
Keyword(s):  
Acid Phosphatase ◽  
Phosphatase Activity ◽  
Acid Phosphatase Activity ◽  
Histochemical Study ◽  
Cerebral Tumors

scholarly journals ACID PHOSPHATASE ACTIVITY IN INDIVIDUAL NEURONS DURING CHROMATOLYSIS: A QUANTITATIVE HISTOCHEMICAL STUDY

1970 ◽  
Vol 18 (11) ◽  
pp. 828-833 ◽  
Author(s):  
HILDE E. HIRSCH ◽  
THEODORE OBENCHAIN
Keyword(s):  
Spinal Cord ◽  
Acid Phosphatase ◽  
Phosphatase Activity ◽  
Acid Phosphatase Activity ◽  
Histochemical Study ◽  
Galactosidase Activity ◽  
Fluorogenic Substrates ◽  
Striking Increase ◽  
Staining Methods ◽  
Glucose 6 Phosphate Dehydrogenase

Two fluorogenic substrates, α-naphthyl phosphate and 4-methylumbelliferyl phosphate, were used to measure acid phosphatase activities in individual anterior horn neurons of the monkey spinal cord after section of the sciatic nerve. Although many studies utilizing staining methods have reported a striking increase in acid phosphatase activity during chromatolysis, no significant differences were observed here between the neurons of the operated and unoperated side, despite widespread chromatolysis. β-galactosidase activity was also unchanged, but the marked elevation of glucose 6-phosphate dehydrogenase during the reparative phase was confirmed.

Erythrophagocytosis in the Liver in Hemolytic Anemias

1968 ◽  
Vol 26 ◽  
pp. 184-185
Author(s):  
O. T. Minick ◽  
E. Orfei ◽  
F. Volini ◽  
G. Kent
Keyword(s):  
Acid Phosphatase ◽  
Oxidative Damage ◽  
Phosphatase Activity ◽  
Kupffer Cells ◽  
Acid Phosphatase Activity ◽  
Common Type ◽  
Red Cell ◽  
Cell Fragments ◽  
Reticulo Endothelial System ◽  
Important Site

Hemolytic anemias were produced in rats by administering phenylhydrazine or anti-erythrocytic (rooster) serum, the latter having agglutinin and hemolysin titers exceeding 1:1000.Following administration of phenylhydrazine, the erythrocytes undergo oxidative damage and are removed from the circulation by the cells of the reticulo-endothelial system, predominantly by the spleen. With increasing dosage or if animals are splenectomized, the Kupffer cells become an important site of sequestration and are greatly hypertrophied. Whole red cells are the most common type engulfed; they are broken down in digestive vacuoles, as shown by the presence of acid phosphatase activity (Fig. 1). Heinz body material and membranes persist longer than native hemoglobin. With larger doses of phenylhydrazine, erythrocytes undergo intravascular fragmentation, and the particles phagocytized are now mainly red cell fragments of varying sizes (Fig. 2).

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