scholarly journals STUDIES IN HISTOCHEMISTRY: XLII FURTHER STUDIES ON THE DETERMINATION OF SUCCINIC DEHYDROGENASE IN MICROGRAM AMOUNTS OF TISSUE AND DISTRIBUTION OF THE ACTIVITY IN THE BOVINE ADRENAL

1956 ◽  
Vol 4 (4) ◽  
pp. 389-396 ◽  
Author(s):  
DAVID GLICK ◽  
SOM N. NAYYAR
Keyword(s):  
Succinic Dehydrogenase

QUANTITATIVE DETERMINATION OF SUCCINIC DEHYDROGENASE AS AN ESTIMATE OF THE FUNCTIONAL STATE OF THE RAT SEMINAL VESICLE

1962 ◽  
Vol 39 (1) ◽  
pp. 129-134
Author(s):  
Antti Telkkä ◽  
A. Kivikoski ◽  
Väinö K. Hopsu
Keyword(s):  
Quantitative Determination ◽  
Functional State ◽  
Quantitative Estimation ◽  
Succinic Dehydrogenase ◽  
Seminal Vesicles ◽  
The Other ◽  
Succinic Dehydrogenase Activity ◽  
Testosterone Treatment ◽  
Rat Seminal Vesicle

ABSTRACT The seminal vesicles of the rat were stimulated by testosterone-treatment or depressed by castration. The activity of the gland was demonstrated using histoquantitative methods and by quantitative determination of the succinic dehydrogenase activity in the vesicles. There was a good correlation between the seven different variables reflecting the functional state of the vesicle. Quantitative estimation of succinic dehydrogenase of the vesicle seemed to be superior to the other methods because of its greater sensitivity.

Histochemical changes occurring at the seedling shoot apex of Pinus radiata

1973 ◽  
Vol 51 (3) ◽  
pp. 501-512 ◽  
Author(s):  
Richard T. Riding ◽  
Ernest M. Gifford Jr.
Keyword(s):  
Pinus Radiata ◽  
Cell Walls ◽  
Succinic Dehydrogenase ◽  
Growing Season ◽  
Peripheral Zone ◽  
Total Proteins ◽  
Epicotyl Elongation ◽  
Histochemical Tests ◽  
Histochemical Changes

Histochemical tests on Pinus radiata shoot apices throughout the growing season showed marked changes in cell constituents. Reserve proteins and lipids were high in dormant embryos. Following imbibition, starch occurred throughout the apex and tannins were evident after the cotyledons emerged from the seed coat. Intensity of stains for DNA, RNA, and nucleohistones increased first at the flanks of the apex. At 84 days DNA, RNA, and histones displayed a zonate distribution pattern. Total proteins and carbohydrates were distributed fairly uniformly.The distribution of enzyme activity also varied. Initially acid phosphatase (AP) and succinic dehydrogenase (SDH) activities were high throughout the apex. A decrease of AP and SDH activity in the apex preceded epicotyl elongation. At 84 days AP activity differed in various regions of the apex; SDH was fairly uniform throughout the central, apical, and peripheral zones. Staining for peroxidase was evident first around aleurone grains and along cell walls after imbibition. With the onset of needle formation, staining was concentrated in subsurface cells. At 84 days peroxidase activity was concentrated in subsurface cells of the peripheral zone and in the rib meristem.The results of this study indicate that the full apex is metabolically active during vegetative growth and all regions are probably involved in the determination of vegetative form.

scholarly journals DETERMINATION OF SUCCINIC DEHYDROGENASE ACTIVITY IN TISSUE CULTURE

1959 ◽  
Vol 7 (2) ◽  
pp. 144-146 ◽  
Author(s):  
NATHAN KAUFMAN ◽  
ROBERT HILL
Keyword(s):  
Tissue Culture ◽  
Dehydrogenase Activity ◽  
Hela Cells ◽  
Succinic Dehydrogenase ◽  
Protective Layer ◽  
The Other ◽  
Tissue Cultures ◽  
Succinic Dehydrogenase Activity

Two methods of demonstrating succinic dehydrogenase activity in tissue cultures of HeLa cells have been demonstrated. One method employs a protective layer of agar applied to the cells prior to incubation in the selected substrate. The other method uses a mixture of agar and substrate which is placed on the cells; incubation follows without the further addition of substrate.

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