scholarly journals DETERMINATION OF SUCCINIC DEHYDROGENASE ACTIVITY IN TISSUE CULTURE

1959 ◽  
Vol 7 (2) ◽  
pp. 144-146 ◽  
Author(s):  
NATHAN KAUFMAN ◽  
ROBERT HILL
Keyword(s):  
Tissue Culture ◽  
Dehydrogenase Activity ◽  
Hela Cells ◽  
Succinic Dehydrogenase ◽  
Protective Layer ◽  
The Other ◽  
Tissue Cultures ◽  
Succinic Dehydrogenase Activity

Two methods of demonstrating succinic dehydrogenase activity in tissue cultures of HeLa cells have been demonstrated. One method employs a protective layer of agar applied to the cells prior to incubation in the selected substrate. The other method uses a mixture of agar and substrate which is placed on the cells; incubation follows without the further addition of substrate.

QUANTITATIVE DETERMINATION OF SUCCINIC DEHYDROGENASE AS AN ESTIMATE OF THE FUNCTIONAL STATE OF THE RAT SEMINAL VESICLE

1962 ◽  
Vol 39 (1) ◽  
pp. 129-134
Author(s):  
Antti Telkkä ◽  
A. Kivikoski ◽  
Väinö K. Hopsu
Keyword(s):  
Quantitative Determination ◽  
Functional State ◽  
Quantitative Estimation ◽  
Succinic Dehydrogenase ◽  
Seminal Vesicles ◽  
The Other ◽  
Succinic Dehydrogenase Activity ◽  
Testosterone Treatment ◽  
Rat Seminal Vesicle

ABSTRACT The seminal vesicles of the rat were stimulated by testosterone-treatment or depressed by castration. The activity of the gland was demonstrated using histoquantitative methods and by quantitative determination of the succinic dehydrogenase activity in the vesicles. There was a good correlation between the seven different variables reflecting the functional state of the vesicle. Quantitative estimation of succinic dehydrogenase of the vesicle seemed to be superior to the other methods because of its greater sensitivity.

Sign in / Sign up

Export Citation Format

Share Document