scholarly journals PEROXIDASE-LABELED ANTIBODY A NEW METHOD OF CONJUGATION

1974 ◽  
Vol 22 (12) ◽  
pp. 1084-1091 ◽  
Author(s):  
PAUL K. NAKANE ◽  
AKIRA KAWAOI
Keyword(s):  
Horseradish Peroxidase ◽  
Free Amino ◽  
Sodium Periodate ◽  
Enzymatic Activities ◽  
Carbohydrate Moiety ◽  
New Method ◽  
Amino Groups ◽  
Aldehyde Groups

A new method of conjugating horseradish peroxidase with proteins was developed. The carbohydrate moiety of fluorodinitrobenzene-blocked peroxidase was oxidized with sodium periodate to form aldehyde groups. The peroxidase-aldehyde was then bound to free amino groups of proteins unidirectionally at high efficiencies. Peroxidase-labeled immunoglobulin retained its immunologic as well as enzymatic activities.

scholarly journals Fabrication of Cellulose Nanocrystal/Chitosan Hydrogel for Controlled Drug Release

Nanomaterials ◽  
2019 ◽  
Vol 9 (2) ◽  
pp. 253 ◽  
Author(s):  
Qinghua Xu ◽  
Yunzhong Ji ◽  
Qiucun Sun ◽  
Yingjuan Fu ◽  
Yongjian Xu ◽  
...  
Keyword(s):  
Drug Release ◽  
Free Amino ◽  
Controlled Drug Release ◽  
Crosslinking Density ◽  
Cellulose Nanocrystal ◽  
Swelling Ratio ◽  
Amino Groups ◽  
Potential Measurement ◽  
Ft Ir ◽  
Aldehyde Groups

In this work, a novel nanocomposite hydrogel based on cellulose nanocrystal (CNC) and chitosan (CS) was fabricated and applied as a carrier for the controlled delivery of theophylline. CNC was firstly periodate-oxidized to obtain dialdehyde nanocellulose (DACNC). Then, chitosan was crosslinked using DACNC as both the matrix and crosslinker in different weight ratios, to fabricate CNC/CS composites. The prepared composites were characterized using Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction pattern (XRD), scanning electron microscopy (SEM), zeta potential measurement and swelling ratio tests. FT-IR results confirmed the successful reaction between the free amino groups on chitosan and the aldehyde groups on DACNC. With the increase of chitosan percentage in the hydrogel, the isoelectric point was shifted towards an alkaline pH, which was probably caused by the higher content of free amino groups. The swelling ratio of the composite also increased, which may have been due to the decrease of crosslinking density. Because the swelling ratio of the drug-loaded hydrogels differed under varied pH values, the cumulative drug release percentage of the composite hydrogel was achieved to approximately 85% and 23% in the gastric (pH 1.5) and intestinal (pH 7.4) fluids, respectively. Therefore, CNC/CS hydrogel has application potential as a theophylline carrier.

Degradation control of cellulose scaffold by Malaprade oxidation

2014 ◽  
Vol 1621 ◽  
pp. 191-196
Author(s):  
Wichchulada Konkumnerd ◽  
Suong-Hyu Hyon ◽  
Kazuaki Matsumura
Keyword(s):  
Amino Acid ◽  
Acid Solution ◽  
Room Temperature ◽  
Sodium Periodate ◽  
Periodate Oxidation ◽  
Oxidized Cellulose ◽  
Amino Groups ◽  
Amino Acid Solution ◽  
Before And After ◽  
Aldehyde Groups

ABSTRACTStudy on oxidizing cellulose scaffold to dialdehyde cellulose by sodium periodate (NaIO4) was carried out. Concentration of sodium periodate and the reaction time were effected for aldehyde introduction to cellulose scaffolds. Cellulose powder was dissolved in 1-butyl-3-methylimidazolium chloride, an ionic liquid, at 100°C and maintained at room temperature for 7 days, providing flexible cellulose scaffold. The cellulose scaffold was oxidized using periodate oxidation (Malaprade oxidation), which oxidizes carbohydrate by glycol cleavage to provide dialdehyde. Aldehyde groups introduced into cellulose were quantified by simple iodometry. Oxidized cellulose scaffold was degraded in the amino acid solution triggered by the reaction between aldehyde groups and amino groups. During immersion of the cellulose scaffolds in the amino acid solution, the mass loss of the scaffolds was evaluated by measuring of weight of oxidized cellulose scaffold before and after degradation.

COMPARATIVE STUDIES OF THE POTENTIATION OF CORTICOTROPHIN ACTIVITY BY SEVERAL INACTIVE DERIVATIVES

1963 ◽  
Vol 42 (2) ◽  
pp. 209-213 ◽  
Author(s):  
Arthur I. Cohen ◽  
Edward H. Frieden
Keyword(s):  
Biological Activity ◽  
Comparative Studies ◽  
Free Amino ◽  
Hormonal Activity ◽  
Amino Groups

ABSTRACT A number of corticotrophin analogues have been prepared, some of which potentiate the biological activity of the untreated hormone in vitro. The free amino groups of corticotrophin appear to be essential not only for hormonal activity, but also for the interaction of the analogues with the tissue corticotrophin inactivating system which is assumed to account for the potentiating effect.

scholarly journals FREE AMINO GROUPS OF EQUINE γ-GLOBULINS AND A SPECIFIC ANTIBODY

1955 ◽  
Vol 216 (2) ◽  
pp. 621-624
Author(s):  
Mary L. McFadden ◽  
Emil L. Smith
Keyword(s):  
Specific Antibody ◽  
Free Amino ◽  
Amino Groups ◽  
Γ Globulins

scholarly journals FREE AMINO GROUPS AND N-TERMINAL SEQUENCE OF RABBIT ANTIBODIES

1955 ◽  
Vol 214 (1) ◽  
pp. 185-196 ◽  
Author(s):  
Mary L. McFadden ◽  
Emil L. Smith
Keyword(s):  
Free Amino ◽  
Terminal Sequence ◽  
Amino Groups

scholarly journals The free amino groups of insulin

1945 ◽  
Vol 39 (5) ◽  
pp. 507-515 ◽  
Author(s):  
F. Sanger
Keyword(s):  
Free Amino ◽  
Amino Groups

scholarly journals Physicochemical properties of three food proteins treated with transglutaminase

2004 ◽  
Vol 34 (4) ◽  
pp. 1219-1223 ◽  
Author(s):  
Luís Henrique de Barros Soares ◽  
Patrícia Melchionna Albuquerque ◽  
Francine Assmann ◽  
Marco Antônio Záchia Ayub
Keyword(s):  
Physicochemical Properties ◽  
Nitrogen Content ◽  
Soy Protein ◽  
Free Amino ◽  
Amino Nitrogen ◽  
Emulsifying Properties ◽  
Amino Groups ◽  
Emulsifying Activity ◽  
Food Proteins ◽  
Isolated Soy Protein

Three sources of food proteins were treated with microbial transglutaminase (EC 2.3.2.13) in order to assess changes in the physicochemical properties of reactivity, solubility, emulsification, and free amino groups of the formed polymers. Samples of lactic casein (LC), isolated soy protein (ISP), and hydrolysed animal protein (HAP), were incubated with the enzyme for one or two hours. LC and ISP showed a reduced solubility of 15% and 24% respectively, with HAP showing no alteration on solubility. Amino nitrogen content was 7%, 3% and 2% reduced for HAP, LC and ISP respectively. LC and ISP demonstrated lower emulsifying activity when they were enzymatically treated but the formed emulsions were stable, contrasting with HAP, which exhibited no changes in emulsifying properties.

Polyacrolein microspheres as a new tool in cell biology

1982 ◽  
Vol 56 (1) ◽  
pp. 157-175
Author(s):  
S. Margel ◽  
U. Beitler ◽  
M. Ofarim
Keyword(s):  
Cell Biology ◽  
Polymerization Process ◽  
Amino Groups ◽  
Ph Values ◽  
Human Red Blood Cells ◽  
Primary Amino ◽  
Aldehyde Groups ◽  
Potential Use ◽  
Specific Labelling ◽  
Reactive Aldehyde

Polyacrolein (PA) microspheres in sizes ranging from 0.04 micron to 40 microns were synthesized. Magnetic and fluorescent PA microspheres were formed by carrying out the polymerization process in the presence of appropriate ferrofluidic or fluorochromic compounds, respectively. The microspheres carry reactive aldehyde groups, through which various ligands, containing primary amino groups, were covalently bound at physiological pH values. The potential use of these microspheres was demonstrated by the specific labelling of fresh human red blood cells (RBC) and by the separation of human RBC from turkey RBC by means of a magnetic field. PA microspheres were also bound covalently to the anti-allergic drug disodium chromoglycate (DSCG) and the conjugate was used for the labelling of rat basophilic leukaemia cells.

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