scholarly journals FLUORESCENT IDENTIFICATION OF Y AND X CHROMATIN BODIES IN HUMAN TISSUES

1973 ◽  
Vol 21 (6) ◽  
pp. 587-591 ◽  
Author(s):  
PETER V. TISHLER ◽  
CARLOS JAVIER
Keyword(s):  
Smooth Muscle ◽  
Human Tissues ◽  
Barr Body ◽  
Male And Female ◽  
Tissue Sections ◽  
Retrospective Assessment ◽  
The Mean ◽  
Formalin Fixed ◽  
Acetic Alcohol

Quinacrine mustard interphase fluorescence may be exploited to study Y and X chromosomal ploidy of many human tissues. Acetic alcohol-fixed cryostat tissue sections are superior, but formalin-fixed sections may also be used after the formalin is removed by vigorous washing in water. The percentage of cells of presumptively euploid female tissues which exhibit Y-like bodies is low, with means of less than l0% of all tissues except brain. In contrast, the mean percentages for euploid male tissues range between 50 and 63% (except for heart) and 30 and 40% when fixed in acetic alcohol and formalin, respectively. The differences between male and female tissues with either method of fixation were statistically significant. Nuclei with two fluorescent bodies were rare. The Barr body could also be recognized in all female tissues, and its presence could be quantitated in nuclei of skeletal and smooth muscle. This method permits the prospective and retrospective assessment of sex chromosomal ploidy, and the correlation of localized tissue karyotype with tissue function in individuals mosaic for aneuploidy of the Y.

Stereological Assessment, Mechanical Measurement and Computer Modelling of Smooth Muscle

2007 ◽  
Vol 567-568 ◽  
pp. 353-356 ◽  
Author(s):  
Zbyněk Tonar ◽  
Petra Kochová ◽  
Miroslav Holeček ◽  
Jiří Janáček
Keyword(s):  
Smooth Muscle ◽  
Elasticity Modulus ◽  
Computer Modelling ◽  
Relative Volume ◽  
Numerical Density ◽  
Mechanical Measurement ◽  
Mean Cell Volume ◽  
Mechanical Measurements ◽  
The Mean ◽  
Formalin Fixed

We used mechanical measurements and unbiased statistical morphometry in order to supply the computer model of smooth muscle cells (SMC) and collagen connective tissue of a gastropod Arion sp. with sufficient input data. We identified the elasticity modulus for both living (34 kPa) and formalin-fixed samples (440 kPa). The relative volume proportion of SMC was 57.8%, their numerical density was 425 925 mm-3, surface density of SMC was 405.9 mm-1 and the mean cell volume was 1 358 .m3.

scholarly journals Study of External Measurements of Heart in Adult Bangladeshi Population

2015 ◽  
Vol 10 (2) ◽  
pp. 78-80
Author(s):  
Lubna Shirin ◽  
Humaira Naushaba ◽  
Mohammad Shahjahan Kabir ◽  
SM Niazur Rahman ◽  
Tahmida Yasmin ◽  
...  
Keyword(s):  
Mean Value ◽  
Cardiac Diseases ◽  
Male And Female ◽  
Medical College ◽  
Standard Data ◽  
Industrialized Nations ◽  
Proper Diagnosis ◽  
The Mean ◽  
Bangladeshi Population ◽  
Formalin Fixed

The knowledge regarding normal physical measurement of heart is very important for proper diagnosis and management of various cardiac diseases. Heart disease is a predominant cause of disability and death among all industrialized nations. This study is to establish a standard data of different external parameters of heart of adult Bangladeshi population. The study was conducted at Department of Anatomy, Sir Salimullah Medical College, Dhaka from July 2009 to December 2009. The formalin fixed cadaveric 60 (sixty) (n=60) human hearts, 41 (forty one) male and 19 (nineteen) female were taken. The length, breadth and weight of the heart were measured and the data was analyzed statistically. The mean value of the length of the heart in male was 10.35±0.62 cm and female was 10.22±0.90 cm. The mean value of the breadth of the heart was 7.45±0.73 cm and 7.35±0.65 cm in male and female respectively. The mean value of the weight of the heart was 174.15±15.49 gm in male, where as for female the mean was 171.58±19.16 gm. The comparison of values of above mentioned variables between male and female were done by unpaired students test and it was statistically not significant.University Heart Journal Vol. 10, No. 2, July 2014; 78-80

scholarly journals The 3-methylhistidine content of human tissues

1979 ◽  
Vol 42 (3) ◽  
pp. 567-570 ◽  
Author(s):  
M. Elia ◽  
A. Carter ◽  
R. Smith
Keyword(s):  
Skeletal Muscle ◽  
Smooth Muscle ◽  
Dry Weight ◽  
Human Tissues ◽  
Post Mortem ◽  
Striated Muscles ◽  
Significant Difference ◽  
Use Values ◽  
Liver And Kidney ◽  
The Mean

1. The amount of 3-methylhistidine (3-MeH) has been measured in eighty-eight samples of tissue taken Post-mortem from five adults.2. The highest concentration (μmol/g fat-free dry weight) of 3-MeH was in skeletal muscle (3.31 ± 0.05); intermediate values (2–3) were found in cardiac muscle and those tissues containing smooth muscle; and low values (less than I) occurred in parenchymal tissues such as liver and kidney.3. There was little variation between the mean 3-MeH content of striated muscles in different individuals, and no significant difference between the 3-MeH concentrations of striated muscles taken from six different sites.4. The results suggest that it is justifiable to use values obtained from single muscles to calculate the rate of myofibrillar breakdown from urinary 3-MeH excretion.

POTRET PERILAKU RELIGIUS MAHASISWA UIN SAYARIF HIDAYATULLAH JAKARTA

Al-Risalah ◽  
2018 ◽  
Vol 8 (2) ◽  
pp. 126-148
Author(s):  
Ay Maryani
Keyword(s):  
Religious Behavior ◽  
Male And Female ◽  
Mean Values ◽  
Significance Level ◽  
Average Value ◽  
Environment Variable ◽  
Independent Variable ◽  
The Mean ◽  
Gender Factor ◽  
Level 2

This study describes the religious behavior of UIN Syarif Hidayatullah Jakarta students. The variables used are internal and external environment as independent variable and religious behavior as dependent variable. The internal environment variable consists of gender factor, (male and female). The external environmental variables comprise the faculty environment, the present resident and the place to grow. The present resident consist of dormitories, boarding houses and parents' homes and the place to grow consist of urban and rural environment. Religious behavior variables consist of habluminallah behavior and habluminannas behavior. Habluminallah's behavior measured by (1) knowledge of faith and worship, (2) attitudes toward faith and worship, and (3) practice of faith and worship. The habluminannas variable measured by (1) Islamic behavior for them self, like honest, discipline and good work / studyethics, trust and concern on legality, (2) Islamic behavior with others, like generous, cooperation, caring, respect to the people's rights and tolerance and (3) Islamic behavior for the natural surroundings, like love of nature and nature conservation efforts. The methodology used was (1) Statistical descriptive, (2) MANOVA (Multivariate Analysis of Variance) and (3) Independent sample t-test. The results showed the religious behavior UIN Syarif Hidayatullah Jakarta students has a very good category. This is indicated by the mean values for the behavior of haluminallah and habluminannas of 158.85 and 178.76,  espectively. The average value of habluminallah behaviour in the range of values "145-180" with the category of "very good" and habluminannas behavior in the range value "165-205" with the category "very good. Habluminallah and habluminannas behavior are different for each faculty. This is indicated by the significance level of Pillai Trace, Wilk Lambda, Hotelling Trace, Roy's Largest Root of 0.00 (<0.05). Habluminallah and habluminannas behavior are the same for respondents who live in dormitories, boarding houses, and parents' homes. This is indicated by the value of F test and significance at Wilk's Lambda respectively for 2.055 and 0.085 (>0.05). Habluminallah and habluminannas behaviors are similar for urban and rural respondents. This is known from the sig level. (2-tailed) for habluminallah and habluminannas behavior of 0.317 and 0.245 (> 0.05), respectively. Habluminallah and habluminannas behaviors are similar for male and female. This is known from the sig level. (2-tailed) for habluminallah and habluminannas behavior of 0.950 and 0.307 (> 0.05),respectively. The results of this study are expected to be used to develop university policies that can enhance the Islamic values of UIN Syarif Hidayatullah Jakarta.

Modern Office Technology Competencies Expected of Office Technology and Management (OTM) Graduate Workers in Delta State.

2019 ◽  
pp. 81-88
Author(s):  
Ozoem Martha ◽  
Chibuike Victoria C. ◽  
Ugwunwoti Emeka P.
Keyword(s):  
Information Processing ◽  
Technology Competencies ◽  
Male And Female ◽  
The World ◽  
Alpha Reliability ◽  
Communication Competencies ◽  
The Mean ◽  
Research Questions ◽  
Level Of Significance ◽  
Descriptive Survey

This study was carried out to determine the modern office technology competencies expected of office technology and management (OTM) graduate workers by supervisors in Delta State. The study was guided by two research questions and two hypotheses tested at 0.05 level of significance. The respondents consisted of 142 supervisors, made up of 74 heads of department and directors of government establishments, and 68 managers and directors of private establishments in the study area. Descriptive survey research design was used to conduct the study and 28 – items questionnaire were used to collect data from respondents. The instrument was validated by three experts and had a Cronbach Alpha reliability coefficient of 0.77. Means with standard deviations were used to answer the research questions, while t-test was used to test the null hypotheses. The extent of supervisor‟s expectations of information processing competencies did not differ significantly based on the mean ratings of male and female supervisors of OTM graduates in government and private establishments. The findings also revealed that supervisors expect much information processing and communication competencies from the OTM graduate workers. Based on the findings and the implications, it was recommended among others that, curriculum planners, business and OTM education lecturers should ensure that the competencies required for modern office technologies are entrenched and taught in the institutions to prepare the OTM graduates for the world of work.

Thomas Aquinas on Sexual Difference: The Metaphysical Biology and Moral Significance of Human Sexuality

Pro Ecclesia ◽  
2021 ◽  
Vol 30 (2) ◽  
pp. 177-215
Author(s):  
Paul Gondreau
Keyword(s):  
Sexual Difference ◽  
Human Sexuality ◽  
Thomas Aquinas ◽  
Moral Significance ◽  
Male And Female ◽  
Biological Design ◽  
Moral Excellence ◽  
Structural Differences ◽  
The Mean ◽  
And Gender

Thomas Aquinas offers for his time a novel take on human sexual difference, in that he grounds human sexuality in what we might term a metaphysical biology and accords it a privileged role in the moral life. Though his biology is drawn from Aristotle, which leads Aquinas to make problematic statements on sexual difference, he nonetheless offers a perspective that remains deeply relevant and significant for today. His method or approach of tethering sexual difference first and foremost to our animal-like biological design remains perennial, particularly at a time when many seek to dismiss biology as irrelevant to sexual identity and gender difference. The latest findings of the emerging field of neurobiology, which have uncovered structural differences between the male and female brains, offer key support to Aquinas’s approach. Even more important, he holds, in an unprecedented move, that sexual design and inclination provide a veritable source of moral excellence. He goes so far as to locate the mean of virtue in our sexual design and appetites.

scholarly journals Discrimination of Aspergillosis, Mucormycosis, Fusariosis, and Scedosporiosis in Formalin-Fixed Paraffin-Embedded Tissue Specimens by Use of Multiple Real-Time Quantitative PCR Assays

2016 ◽  
Vol 54 (11) ◽  
pp. 2798-2803 ◽  
Author(s):  
Elham Salehi ◽  
Mohammad T. Hedayati ◽  
Jan Zoll ◽  
Haleh Rafati ◽  
Maryam Ghasemi ◽  
...  
Keyword(s):  
Fusarium Oxysporum ◽  
Aspergillus Flavus ◽  
Ffpe Tissue ◽  
Content Type ◽  
Real Time Quantitative Pcr ◽  
Tissue Sections ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Tissue Specimens ◽  
Formalin Fixed

In a retrospective multicenter study, 102 formalin-fixed paraffin-embedded (FFPE) tissue specimens with histopathology results were tested. Two 4- to 5-μm FFPE tissue sections from each specimen were digested with proteinase K, followed by automated nucleic acid extraction. Multiple real-time quantitative PCR (qPCR) assays targeting the internal transcribed spacer 2 (ITS2) region of ribosomal DNA, using fluorescently labeled primers, was performed to identify clinically important genera and species of Aspergillus , Fusarium , Scedosporium , and the Mucormycetes . The molecular identification was correlated with results from histological examination. One of the main findings of our study was the high sensitivity of the automated DNA extraction method, which was estimated to be 94%. The qPCR procedure that was evaluated identified a range of fungal genera/species, including Aspergillus fumigatus , Aspergillus flavus , Aspergillus terreus , Aspergillus niger , Fusarium oxysporum , Fusarium solani , Scedosporium apiospermum , Rhizopus oryzae , Rhizopus microsporus , Mucor spp., and Syncephalastrum . Fusarium oxysporum and F. solani DNA was amplified from five specimens from patients initially diagnosed by histopathology as having aspergillosis. Aspergillus flavus , S. apiospermum , and Syncephalastrum were detected from histopathological mucormycosis samples. In addition, examination of four samples from patients suspected of having concomitant aspergillosis and mucormycosis infections resulted in the identification of two A. flavus isolates, one Mucor isolate, and only one sample having both R. oryzae and A. flavus . Our results indicate that histopathological features of molds may be easily confused in tissue sections. The qPCR assay used in this study is a reliable tool for the rapid and accurate identification of fungal pathogens to the genus and species levels directly from FFPE tissues.

scholarly journals The differential transmissibility of myxoma virus strains of differing virulence grades by the rabbit flea Spilopsyllus cuniculi (Dale)

1975 ◽  
Vol 75 (2) ◽  
pp. 237-247 ◽  
Author(s):  
A. R. Mead-Briggs ◽  
J. A. Vaughan
Keyword(s):  
Host Resistance ◽  
Oryctolagus Cuniculus ◽  
Selective Advantage ◽  
Myxoma Virus ◽  
Laboratory Studies ◽  
Natural Conditions ◽  
Male And Female ◽  
The Mean ◽  
Virus Strains ◽  
Sampling Times

SUMMARYLaboratory studies showed that few rabbit fleas (Spilopsyllus cuniculi (Dale)) transmitted myxomatosis after removal from wild rabbits (Oryctolagus cuniculus (L)) that had been infected for fewer than 10–12 days, irrespective of the virulence of the myxoma virus strain involved. Rabbits infected with fully virulent (Grade I) strains died within 10–15 days and few fleas from these hosts became infective; averaging all the samples taken, 12% of the fleas were infective. Also, few fleas acquired infectivity on individual rabbits which recovered from infection with attenuated strains; the mean was 8% infective. Rabbits which died between 17 and 44 days after infection had higher proportions of infective fleas at all sampling times; the mean was 42% infective. Male and female fleas transmitted virus with equal efficiency.For rabbits infected with any of the attenuated virus strains the mean percentage of infective fleas was inversely related to the survival time of the host. Rabbits infected with moderately attenuated strains (Grades IIIA and IIIB) had, on average, the highest proportion of infective fleas; hence such strains have a selective advantage and have become predominant under natural conditions in Britain. The changes that might occur if there is an increase in host resistance to myxomatosis are discussed.

Sign in / Sign up

Export Citation Format

Share Document