Localization of Epstein-Barr Virus-Encoded Small RNA-1 by in situ Reverse Transcription: Demonstration of cDNA Generation in Formalin-Fixed Paraffin-Embedded Tissue Sections

1995 ◽  
Vol 2 (3) ◽  
pp. 249-255
Author(s):  
Tzyy-Choou Wu ◽  
Yin Ling ◽  
Masahide D. Kanayama ◽  
Alex Y.C. Huang ◽  
Robert J. Kurman
Keyword(s):  
Small Rna ◽  
Reverse Transcription ◽  
Epstein Barr Virus ◽  
Barr Virus ◽  
Tissue Sections ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Epstein Barr ◽  
Formalin Fixed

scholarly journals Determination of the Expression of Latent Epstein Barr Virus in Omani Nasopharyngeal Carcinoma Patients

2021 ◽  
Vol 14 (1) ◽  
pp. 257-265
Author(s):  
Sheikha Nasser said Al-Shidhani ◽  
Shadia Al-Sinawi ◽  
Maiya Al-Bahri ◽  
Masoud Al-Kindi ◽  
Mohamed Mabruk
Keyword(s):  
Nasopharyngeal Carcinoma ◽  
Epstein Barr Virus ◽  
Tissue Samples ◽  
Barr Virus ◽  
Carcinoma Tissue ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Epstein Barr ◽  
Formalin Fixed

Background: Nasopharyngeal carcinoma(NPC) is a rare malignant carcinoma that develops in the epithelial lining of the nasopharyngeal mucosa It is the most common neoplasm of the Nasopharynxand it is associated with many risk factors; one of them is Epstein-Barr virus infection. An Epstein-Barr virus is a tumorigenic herpes virus that infects and persists in B-lymphocytes without causing disease. This virus is associated with significant pathological conditions, such as benign and malignant lymphoproliferation. Objectives: To determine Epstein -Barr encoded RNA 1&2 (EBER1,2) and latent membrane protein (LMP) expressionin formalin-fixed paraffin-embedded tissue samples obtained from Omani patients diagnosed with nasopharyngeal carcinoma.Also, to identify the pattern and the type(s) of cells infected with EBV in nasopharyngeal carcinoma tissue samples obtained from Omani patients. Moreover, to compare the sensitivity of Immuno histochemistry and in-situ Hybridization for the detection of EBV in the nasopharyngeal carcinoma tissue sample. Materials and Method:Thirteen formalin-fixed paraffin-embedded nasopharyngeal carcinoma tissue samples archived from the period 2010 to 2017, were obtained from the Pathology Departments of Sultan Qaboos University Hospital and the Armed Force Hospital. These tissue samples were processed using two different methods Immunohistochemistry (IHC) and In situ hybridization (ISH). Results:Eleven out of thirteen NPC Omani patients were positive for EBV (84.61%) by either LMP-IHC or EBER-ISH. All cells stained positive for EBV in NPC tissue samples was of malignant type rather than normal cell type. EBV is mostly detected in patients in the age group of less than 50 years old. Also out of the 13 NPC patients, seven females (58.34%), and six males (46.15%) were positive for EBV. Conclusion:This study may provide evidence indicating an association between EBV and nasopharyngeal carcinoma. In addition, the detection of EBV in NPC obtained from Omani patients may encourage the physician to consider using anti-herpes virus drugs in the treatment of EBV positive NPC patients as an additional tool for the treatment of this kind of malignancy.

scholarly journals Frequency of Epstein–Barr Virus DNA in Formalin-fixed Paraffin-embedded Tissue of Patients with Ductal Breast Carcinoma

2019 ◽  
Vol 20 (3) ◽  
pp. 687-692 ◽  
Author(s):  
Chia Sharifpour ◽  
Manoochehr Makvandi ◽  
Alireza Samabafzadeh ◽  
Abdolhassan Talaei-Zadeh ◽  
Nastaran Ranjbari ◽  
...  
Keyword(s):  
Breast Carcinoma ◽  
Epstein Barr Virus ◽  
Barr Virus ◽  
Ductal Breast Carcinoma ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Epstein Barr ◽  
Formalin Fixed

scholarly journals High prevalence of Epstein-Barr virus in the Reed-Sternberg cells of Hodgkin's disease occurring in Peru

Blood ◽  
1993 ◽  
Vol 81 (2) ◽  
pp. 496-501 ◽  
Author(s):  
KL Chang ◽  
PF Albujar ◽  
YY Chen ◽  
RM Johnson ◽  
LM Weiss
Keyword(s):  
In Situ Hybridization ◽  
Epstein Barr Virus ◽  
Lower Percentage ◽  
Double Labeling ◽  
Barr Virus ◽  
Formalin Fixed Paraffin ◽  
High Prevalence ◽  
Formalin Fixed Paraffin Embedded ◽  
Epstein Barr

Abstract The Epstein-Barr virus (EBV) has been implicated in the pathogenesis of Hodgkin‧s disease (HD). This study was undertaken to determine whether the association of EBV with HD showed geographical variation, as in Burkitt‧s lymphoma. We studied 32 formalin-fixed, paraffin-embedded cases of HD occurring in Peru. EBV DNA-RNA in situ hybridization was performed using a 30-base biotinylated antisense oligonucleotide complementary to the EBER1 gene of EBV. EBV immunohistochemistry was also performed, using a monoclonal antibody (MoAb) to the latent membrane protein (LMP1) of EBV. Identification of the precise cellular subset staining with EBV was accomplished via double-labeling with MoAbs directed against Reed-Sternberg cells (LeuM1/CD15) and B cells (L26/CD20). EBV RNA was identified in all or virtually all of the Reed- Sternberg cells and variants in 30 of the 32 (94%) cases of HD by in situ hybridization. LMP1 expression was identified in 83% of the EBER1- positive cases. Double-labeling studies confirmed the localization of EBV RNA to CD15-expressing Hodgkin‧s cells. This study found an extremely high prevalence of EBV in Peruvian HD, in contrast to the much lower percentage of EBV-associated cases of HD occurring in “Western” patients.

scholarly journals Detection of Epstein Barr virus in formalin-fixed paraffin tissues by fluorescent direct in situ PCR

10.4081/958 ◽  
2009 ◽  
Vol 49 (3) ◽  
pp. 309
Author(s):  
N Marziliano ◽  
E Arbustini ◽  
M Rossi de Gasperis ◽  
S Crovella
Keyword(s):  
Epstein Barr Virus ◽  
In Situ Pcr ◽  
Barr Virus ◽  
Formalin Fixed Paraffin ◽  
Epstein Barr ◽  
Formalin Fixed

scholarly journals High prevalence of Epstein-Barr virus in the Reed-Sternberg cells of Hodgkin's disease occurring in Peru

Blood ◽  
1993 ◽  
Vol 81 (2) ◽  
pp. 496-501 ◽  
Author(s):  
KL Chang ◽  
PF Albujar ◽  
YY Chen ◽  
RM Johnson ◽  
LM Weiss
Keyword(s):  
In Situ Hybridization ◽  
Epstein Barr Virus ◽  
Lower Percentage ◽  
Double Labeling ◽  
Barr Virus ◽  
Formalin Fixed Paraffin ◽  
High Prevalence ◽  
Formalin Fixed Paraffin Embedded ◽  
Epstein Barr

The Epstein-Barr virus (EBV) has been implicated in the pathogenesis of Hodgkin‧s disease (HD). This study was undertaken to determine whether the association of EBV with HD showed geographical variation, as in Burkitt‧s lymphoma. We studied 32 formalin-fixed, paraffin-embedded cases of HD occurring in Peru. EBV DNA-RNA in situ hybridization was performed using a 30-base biotinylated antisense oligonucleotide complementary to the EBER1 gene of EBV. EBV immunohistochemistry was also performed, using a monoclonal antibody (MoAb) to the latent membrane protein (LMP1) of EBV. Identification of the precise cellular subset staining with EBV was accomplished via double-labeling with MoAbs directed against Reed-Sternberg cells (LeuM1/CD15) and B cells (L26/CD20). EBV RNA was identified in all or virtually all of the Reed- Sternberg cells and variants in 30 of the 32 (94%) cases of HD by in situ hybridization. LMP1 expression was identified in 83% of the EBER1- positive cases. Double-labeling studies confirmed the localization of EBV RNA to CD15-expressing Hodgkin‧s cells. This study found an extremely high prevalence of EBV in Peruvian HD, in contrast to the much lower percentage of EBV-associated cases of HD occurring in “Western” patients.

Application of in situ hybridization with a novel phenytoin-labeled probe to conventional formalin-fixed, paraffin-embedded tissue sections

1995 ◽  
Vol 52 (3) ◽  
pp. 309-316 ◽  
Author(s):  
Yoshito Eizuru ◽  
Yoichi Minamishima ◽  
Tadashi Matsumoto ◽  
Toshinari Hamakado ◽  
Mikio Mizukoshi ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Tissue Sections ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Labeled Probe ◽  
Formalin Fixed
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