scholarly journals QUANTITATIVE HISTOCHEMICAL ANALYSIS OF GLYCOLYTIC INTERMEDIATES AND COFACTORS WITH AN OIL WELL TECHNIQUE

1968 ◽  
Vol 16 (1) ◽  
pp. 29-39 ◽  
Author(s):  
F. M. MATSCHINSKY ◽  
J. V. PASSONNEAU ◽  
O. H. LOWRY

Quantitative histochemical measurements of glycolytic intermediates and cofactors in samples of the order of 0.1 µg dry weight have been made possible by the use of enzymatic fluorometric methods in combination with the technique of enzymatic cycling. In order to prevent evaporation of the necessarily small volumes of fluid and to avoid exposure to CO2 in the air, incubations are carried out under oil in wells drilled in Teflon. The general technique for conducting analyses in oil wells is described together with specific procedures for measuring glycogen, glucose, glucose 6-phosphate, uridine diphosphoglucose, fructose diphosphate, lactate, adenosine triphosphate, phosphocreatine and inorganic phosphate. The potential of the technique is illustrated by measurements of these compounds in each of nine discrete layers of the retina.

2021 ◽  
Author(s):  
Robert Downey ◽  
Kiran Venepalli ◽  
Jim Erdle ◽  
Morgan Whitelock

Abstract The Permian Basin of west Texas is the largest and most prolific shale oil producing basin in the United States. Oil production from horizontal shale oil wells in the Permian Basin has grown from 5,000 BOPD in February, 2009 to 3.5 Million BOPD as of October, 2020, with 29,000 horizontal shale oil wells in production. The primary target for this horizontal shale oil development is the Wolfcamp shale. Oil production from these wells is characterized by high initial rates and steep declines. A few producers have begun testing EOR processes, specifically natural gas cyclic injection, or "Huff and Puff", with little information provided to date. Our objective is to introduce a novel EOR process that can greatly increase the production and recovery of oil from shale oil reservoirs, while reducing the cost per barrel of recovered oil. A superior shale oil EOR method is proposed that utilizes a triplex pump to inject a solvent liquid into the shale oil reservoir, and an efficient method to recover the injectant at the surface, for storage and reinjection. The process is designed and integrated during operation using compositional reservoir simulation in order to optimize oil recovery. Compositional simulation modeling of a Wolfcamp D horizontal producing oil well was conducted to obtain a history match on oil, gas, and water production. The matched model was then utilized to evaluate the shale oil EOR method under a variety of operating conditions. The modeling indicates that for this particular well, incremental oil production of 500% over primary EUR may be achieved in the first five years of EOR operation, and more than 700% over primary EUR after 10 years. The method, which is patented, has numerous advantages over cyclic gas injection, such as much greater oil recovery, much better economics/lower cost per barrel, lower risk of interwell communication, use of far less horsepower and fuel, shorter injection time, longer production time, smaller injection volumes, scalability, faster implementation, precludes the need for artificial lift, elimination of the need to buy and sell injectant during each cycle, ability to optimize each cycle by integration with compositional reservoir simulation modeling, and lower emissions. This superior shale oil EOR method has been modeled in the five major US shale oil plays, indicating large incremental oil recovery potential. The method is now being field tested to confirm reservoir simulation modeling projections. If implemented early in the life of a shale oil well, its application can slow the production decline rate, recover far more oil earlier and at lower cost, and extend the life of the well by several years, while precluding the need for artificial lift.


2014 ◽  
Vol 692 ◽  
pp. 433-438 ◽  
Author(s):  
Jing Fu Zhang ◽  
Jin Long Yang ◽  
Kai Liu ◽  
Bo Wang ◽  
Rui Xue Hou

Carbon dioxide CO2could corrode the oil well cement paste matrix under agreeable moisture and pressure condition in deep oil wells, which could decrease the compressive strength and damage the annular seal reliability of cement paste matrix. The problem of oil well cement paste matrix corrosion by CO2was researched in the paper for obtain the feasible corrosion prevention technical measures. The microstructure and compressive strength of corroded cement paste matrix were examined by scanning electron microscopeSEMand strength test instrument etc. under different corrosion conditions. The mechanism and effect law of corrosion on oil well cement paste matrix by CO2were analyzed. And the suitable method to protect CO2corrosion in deep oil wells was explored. The results show that the corrosion mechanism of cement paste matrix by CO2was that the wetting phase CO2could generate chemical reaction with original hydration products produced from cement hydration, which CaCO3were developed and the original composition and microstructure of cement paste matrix were destroyed. The compressive strength of corrosion cement paste matrix always was lower than that of un-corrosion cement paste matrix. The compressive strength of corrosion cement paste matrix decreased with increase of curing temperature and differential pressure. The corroded degree of cement paste matrix was intimately related with the compositions of cement slurry. Developing and design anti-corrosive cement slurry should base on effectively improving the compact degree and original strength of cement paste matrix. The compounding additive R designed in the paper could effectively improve the anti-corrosive ability of cement slurry.


1943 ◽  
Vol 10 (1) ◽  
pp. A1-A12
Author(s):  
B. F. Langer ◽  
E. H. Lamberger

Abstract The sucker-rod pump as used in oil wells is treated as a problem in the longitudinal vibration of bars. Solutions are obtained for the forces and motions at both ends of the rod string, thus giving formulas for the calculation of polished-rod load and plunger travel. The results of the calculations are compared with test results.


1962 ◽  
Vol 202 (1) ◽  
pp. 77-79 ◽  
Author(s):  
Richard N. Lolley ◽  
Frederick E. Samson

Acid-soluble phosphates of rat brain during anoxia were determined by ion-exchange and chemical procedures. There is a general shift during anoxia of triphosphate nucleotides to monophosphates and a very rapid breakdown of phosphoryl-creatine. However, total phosphate leaving the high-energy phosphate pool is not equal to the changes in inorganic phosphate; inorganic phosphate change is much larger than high-energy phosphate change in early anoxia and much smaller in extended anoxia. The patterns of guanosine triphosphate and uridine triphosphate changes are more complex than adenosine triphosphate changes. Nicotinamideadenine dinucleotide levels are steady until late anoxia, at which time they decrease slightly. Cytidine monophosphate is the only cytidine nucleotide detected. Inosine nucleotide concentrations in control animals were below the limit of the method, but in late anoxia inosine monophosphate appeared. The data show that the energy flow through the phosphates in brain is rapid and involves phosphate compounds other than the acid-soluble nucleotides and phosphoryl-creatine.


2020 ◽  
Vol 12 (01) ◽  
pp. 2050001
Author(s):  
Yadigar N. Imamverdiyev ◽  
Fargana J. Abdullayeva

In this paper, a fault prediction method for oil well equipment based on the analysis of time series data obtained from multiple sensors is proposed. The proposed method is based on deep learning (DL). For this purpose, comparative analysis of single-layer long short-term memory (LSTM) with the convolutional neural network (CNN) and stacked LSTM methods is provided. To demonstrate the efficacy of the proposed method, some experiments are conducted on the real data set obtained from eight sensors installed in oil wells. In this paper, compared to the single-layer LSTM model, the CNN and stacked LSTM predicted the faulty time series with a minimal loss.


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