Light-Induced Dark [32P]Adenosine Triphosphate Formation by Rhodospirillum rubrum Chromatophores. Adenosine Triphosphate-Inorganic Phosphate Exchange Activity*

Biochemistry ◽  
1966 ◽  
Vol 5 (1) ◽  
pp. 34-40 ◽  
Author(s):  
W. S. Zaugg ◽  
L. P. Vernon
Keyword(s):  
Adenosine Triphosphate ◽  
Inorganic Phosphate ◽  
Rhodospirillum Rubrum ◽  
Phosphate Exchange ◽  
Exchange Activity

scholarly journals STUDIES OF PHOSPHORUS METABOLISM IN MAN: II. A STUDY OF THE PERMEABILITY OF THE HUMAN ERYTHROCYTE TO INORGANIC PHOSPHATE IN VITRO BY THE USE OF RADIOACTIVE PHOSPHATE (P32)

Blood ◽  
1948 ◽  
Vol 3 (12) ◽  
pp. 1472-1477 ◽  
Author(s):  
F. H. L. TAYLOR ◽  
S. M. LEVENSON ◽  
M. A. ADAMS ◽  
MARY KENDRICK
Keyword(s):  
Human Erythrocyte ◽  
Inorganic Phosphate ◽  
Sodium Phosphate ◽  
Specific Activity ◽  
Soluble Fraction ◽  
High Specific Activity ◽  
Phosphorus Metabolism ◽  
Phosphate Exchange ◽  
Inorganic Fraction

Abstract 1. Phosphate exchange in red cells and plasma was studied in vitro using P32 in the form of sodium phosphate as a tracer. 2. No phosphate was added other than the isotopic preparation which was of high specific activity. 3. Inorganic phosphate exchanged freely between the plasma and the erythrocytes at 37.5 C. in a period of four hours. Minimal transfer occurred at 7 C. 4. Most of the added P32 which passed into the erythrocytes during this time remained in the inorganic fraction, less than 15 per cent being found in the organic acid soluble fraction. 5. The specific activity of the inorganic phosphate of the erythrocytes was equal to or greater than that obtaining for the inorganic phosphate of the plasma at the end of the four hour incubation period at 37.5 C.

scholarly journals QUANTITATIVE HISTOCHEMICAL ANALYSIS OF GLYCOLYTIC INTERMEDIATES AND COFACTORS WITH AN OIL WELL TECHNIQUE

1968 ◽  
Vol 16 (1) ◽  
pp. 29-39 ◽  
Author(s):  
F. M. MATSCHINSKY ◽  
J. V. PASSONNEAU ◽  
O. H. LOWRY
Keyword(s):  
Adenosine Triphosphate ◽  
Inorganic Phosphate ◽  
Dry Weight ◽  
Oil Wells ◽  
Oil Well ◽  
Histochemical Analysis ◽  
General Technique ◽  
Enzymatic Cycling ◽  
Glycolytic Intermediates

Quantitative histochemical measurements of glycolytic intermediates and cofactors in samples of the order of 0.1 µg dry weight have been made possible by the use of enzymatic fluorometric methods in combination with the technique of enzymatic cycling. In order to prevent evaporation of the necessarily small volumes of fluid and to avoid exposure to CO2 in the air, incubations are carried out under oil in wells drilled in Teflon. The general technique for conducting analyses in oil wells is described together with specific procedures for measuring glycogen, glucose, glucose 6-phosphate, uridine diphosphoglucose, fructose diphosphate, lactate, adenosine triphosphate, phosphocreatine and inorganic phosphate. The potential of the technique is illustrated by measurements of these compounds in each of nine discrete layers of the retina.

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