scholarly journals MODIFIED PROCEDURE FOR THE HISTOCHEMICAL LOCALIZATION OF RIBONUCLEASE ACTIVITY BY THE SUBSTRATE FILM METHOD

1966 ◽  
Vol 14 (3) ◽  
pp. 254-259 ◽  
Author(s):  
ROGER DAOUST
Keyword(s):  
Original Method ◽  
Ribonuclease Activity ◽  
Rnase Activity ◽  
Histochemical Localization ◽  
Rat Tissues ◽  
Tissue Sections ◽  
Original Procedure ◽  
Film Method ◽  
Substrate Film

The substrate film method for localizing RNAse activity has been the object of further studies, and various steps of the original procedure have been modified. The modifications concern mainly the type of RNA used as substrate and the preparation of gelatin-RNA films. Moreover, a technique proposed by other authors for exposing films to tissue sections was found to present interesting advantages and has been adopted. The modified procedure proved more convenient than the original method, and a higher resolution was achieved in the present work. This report describes the revised procedure for localizing RNAse activity in tissue sections, exposes the advantages of the various modifications and presents the results obtained with different rat tissues, namely, the testis, intestine, brain, kidney, pancreas and ovary.

scholarly journals THE EFFECT OF COENZYME Q ON THE HISTOCHEMICAL SUCCINIC TETRAZOLIUM REDUCTASE REACTION: A HISTOCHEMICAL STUDY

1967 ◽  
Vol 15 (4) ◽  
pp. 216-224 ◽  
Author(s):  
CHARLES A. HORWITZ ◽  
LUIS BENITEZ ◽  
MARGARET BRAY
Keyword(s):  
Histochemical Study ◽  
Coenzyme Q ◽  
Succinic Dehydrogenase ◽  
Test System ◽  
Rat Tissues ◽  
Tissue Sections ◽  
Normal Rat ◽  
Electron Carriers ◽  
Cryostat Sections ◽  
Film Method

The role played by coenzyme Q (CoQ) in the succinic tetrazolium reductase reaction was investigated. Fresh cryostat sections of normal rat tissues were extracted with acetone to remove CoQ from the tissue sections and the acetone-extracted sections were reconstituted with CoQ-lecithin complexes. The incubation film method for tetrazolium reductases was used as a test system, using succinate as a substrate. Sections from which CoQ had been selectively extracted did not reduce the tetrazolium to its formazan, whereas those acetone-extracted sections that were treated with CoQ-lecithin complexes or with the electron carrier phenazine methosulfate reacted positively. It was concluded that the succinic tetrazolium reductase reaction requires intermediate electron carriers, mainly CoQ. It follows that nitro blue tetrazolium cannot accept electrons directly from the flavins. Therefore, the tetrazolium reductase reaction depends not only on the amount of succinic dehydrogenase and flavins but also on the amount of CoQ in the tissue.

scholarly journals Relationship between formation of gametophore buds in the protonema of mosses and increase in ribonuclease activity

2015 ◽  
Vol 44 (3) ◽  
pp. 433-441
Author(s):  
M. Spychała ◽  
J. Schneider ◽  
A. Szeykowska
Keyword(s):  
Enzyme Activity ◽  
Early Phase ◽  
Ribonuclease Activity ◽  
Rnase Activity ◽  
Bud Formation ◽  
Factors Affecting ◽  
Spontaneous Formation

Changes in RNase activity similar to those accompanying cytokinin-induced formation of gametophore buds in mosses (a decrease in the early phase of bud formation and later an increase in enzyme activity) have also been found during spontaneous formation of gametophores in moss ontogenesis. Using various factors affecting the cytokinin-induced process of bud formation a correlation has been found between this process and the increase in RNase activity.

Histochemical localization of mushroom tyrosinase in whole tissue sections on nitrocellulose

1989 ◽  
Vol 90 (5) ◽  
pp. 379-381 ◽  
Author(s):  
B. M. Moore ◽  
B. Kang ◽  
W. H. Flurkey
Keyword(s):  
Histochemical Localization ◽  
Mushroom Tyrosinase ◽  
Tissue Sections

RIBONUCLEASE ACTIVITY IN RUSTED WHEAT LEAVES

1961 ◽  
Vol 39 (4) ◽  
pp. 775-784 ◽  
Author(s):  
R. Rohringer ◽  
D. J. Samborski ◽  
C. O. Person
Keyword(s):  
Leaf Rust ◽  
Direct Evidence ◽  
Specific Activity ◽  
Host Tissue ◽  
Ribonuclease Activity ◽  
Rnase Activity ◽  
Rnase Inhibitor ◽  
Germination Medium ◽  
Wheat Leaves ◽  
Host Tissues

Extracts from primary leaves of Lee wheat were prepared at various days following inoculation with races of leaf rust and tested for ribonuclease (RNase) activity. As early as 24 hours after inoculation there was a marked increase in the specific activity of the enzyme in extracts of rusted host tissues. A further increase in activity was observed during later stages of infection, with the susceptible and resistant reacting tissue differing only in the degree of their response. Extracts from noninoculated control leaves exhibited a constant RNase activity throughout the period of observation. The germination medium and extracts from germinating uredospores contained comparatively little RNase activity. No direct evidence was obtained either for the possible release of the enzyme from particulate cellular fractions of the host tissue as a result of infection or for the removal of an RNase inhibitor in the host tissue responding to infection.

scholarly journals The distribution of acid and alkaline ribonuclease activities in preneoplastic and neoplastic rat livers.

1977 ◽  
Vol 25 (2) ◽  
pp. 115-121 ◽  
Author(s):  
S M Murthy ◽  
R Daoust
Keyword(s):  
Ribonucleic Acid ◽  
Serial Sections ◽  
Parenchymal Tissue ◽  
Precipitation Technique ◽  
Stromal Tissue ◽  
Alkaline Ribonuclease ◽  
Rat Livers ◽  
Film Method ◽  
Substrate Film

Ribonuclease (RNase) activities revealed by the substrate film method were compared with reactions for acid and alkaline RNase obtained by lead precipitation technique in serial sections of preneoplastic livers and hepatomas. The preneoplastic parenchymal tissue giving positive reactions with ribonucleic acid films showed both acid and alkaline RNase activities by lead precipitation technique, and the area of hyperplastic nodules nonreactive against substrate films were deficient in acid and alkaline RNase activities. Preneoplastic hyperbasophilic foci and hepatoma gave weak or negative reactions by either method, but necrotic areas and stromal tissue showed appreciable RNase activities. Thus a good correlation was observed in these tissues between the RNase activities revealed by the film method and those demonstrated by lead precipitation.

Tissue distribution of sulfolipids in the rat. Restricted location of sulfatoxygalactosylacylalkylglyerol

1981 ◽  
Vol 59 (7) ◽  
pp. 556-563 ◽  
Author(s):  
Clifford Lingwood ◽  
Genevieve Hay ◽  
Harry Schachter
Keyword(s):  
Tissue Distribution ◽  
Indirect Immunofluorescence ◽  
Rat Tissues ◽  
Tissue Sections ◽  
Frozen Tissue

Eleven rat tissues (excluding brain) have been assayed for their ability to synthesize sulfatoxygalactosylacylalkylglycerol (SGG) from Na235SO4in vivo. These tissues were also assayed for the presence of SGG by an indirect immunofluorescence procedure using rabbit anti-SGG and frozen tissue sections. By both procedures SGG was found to be restricted to the testis; several novel sulfolipids were detected during this study.

scholarly journals REDUCTION OF HEATING ARTIFACTS IN THIN SECTIONS EXAMINED IN THE ELECTRON MICROSCOPE

1957 ◽  
Vol 3 (6) ◽  
pp. 1017-1022 ◽  
Author(s):  
Michael L. Watson
Keyword(s):  
Electron Beam ◽  
Electron Microscope ◽  
High Melting ◽  
High Melting Point ◽  
Tissue Mass ◽  
Thin Sections ◽  
Tissue Sections ◽  
Reduce Surface Tension ◽  
Substrate Film ◽  
General Appearance

Certain phenomena affecting contrast obtained from tissue sections with the electron microscope have been investigated and a technique is described for reducing destruction by the electron beam of fine details in sections. It has been concluded that loss of embedding material is slightly higher at exposed surfaces of sections than it is at surfaces covered by substrate film. Covering of both surfaces of sections with thin films of formvar, collodion, or carbon materially improves the general appearance, reduces distortion, and sometimes reduces loss of tissue mass from the section as result of exposure to the electron beam. This improvement is considered to result from the relatively high melting-point of the covering films which serve to eliminate or reduce surface-tension or other forces operating in methacrylate softened by the electron beam.

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