Tissue distribution of sulfolipids in the rat. Restricted location of sulfatoxygalactosylacylalkylglyerol

1981 ◽  
Vol 59 (7) ◽  
pp. 556-563 ◽  
Author(s):  
Clifford Lingwood ◽  
Genevieve Hay ◽  
Harry Schachter
Keyword(s):  
Tissue Distribution ◽  
Indirect Immunofluorescence ◽  
Rat Tissues ◽  
Tissue Sections ◽  
Frozen Tissue

Eleven rat tissues (excluding brain) have been assayed for their ability to synthesize sulfatoxygalactosylacylalkylglycerol (SGG) from Na235SO4in vivo. These tissues were also assayed for the presence of SGG by an indirect immunofluorescence procedure using rabbit anti-SGG and frozen tissue sections. By both procedures SGG was found to be restricted to the testis; several novel sulfolipids were detected during this study.

Timing of sulphogalactolipid biosynthesis in the rat testis studied by tissue autoradiography

1985 ◽  
Vol 75 (1) ◽  
pp. 329-338
Author(s):  
C.A. Lingwood
Keyword(s):  
Germ Cell ◽  
Pachytene Stage ◽  
Early Pachytene ◽  
Rat Testis ◽  
Tissue Sections ◽  
Metabolic Labelling ◽  
Frozen Tissue

The testicular synthesis of sulphatoxygalactosylacylalkylglycerol (SGG) has been studied in the rat by autoradiography of frozen tissue sections following in vivo metabolic labelling. The results are consistent with the synthesis of this major mammalian germ-cell glycolipid at the zygotene and early pachytene stages of spermatogenesis. Further synthesis of SGG is prevented by the appearance of an inhibitor of galactolipid sulphotransferase activity at the mid-pachytene stage.

scholarly journals The Tissue Distribution of Four Major Coumarins after Oral Administration of Angelicae Pubescentis Radix Extract to Rats Using Ultra-High-Performance Liquid Chromatography

2019 ◽  
Vol 2019 ◽  
pp. 1-8 ◽  
Author(s):  
Yuanyuan Ge ◽  
Shujing Chen ◽  
Qian Luo ◽  
Chun-peng Wang ◽  
Jia Hao ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Oral Administration ◽  
Tissue Distribution ◽  
High Performance ◽  
Ultra Performance Liquid Chromatography ◽  
Tissue Homogenate ◽  
Rat Tissues ◽  
Relative Standard ◽  
Limit Of Quantitation

Angelicae pubescentis radix (APR) is widely applied in treating rheumatoid arthritis in China. Coumarins are the major active compounds of APR extract including columbianetin, columbianetin acetate, osthole, and columbianadin. The in vivo behavior of the four major coumarins of APR has not been systematically reported. A feasible and reliable ultra-performance liquid chromatography (UPLC) method was established and validated for the quantification of the above four coumarins in rat various tissues (including heart, liver, spleen, lung, kidney, uterus, ovary, and muscle) after oral administration of APR extract. The separation was implemented on a Waters ACQUITY BEH C18 column (4.6 mm × 100 mm, 1.7 μm) with gradient mobile phase comprising acetonitrile-water (with 1mM formic acid) at a flow rate of 0.3 mL/min. The tissue homogenate samples were prepared by liquid-liquid extraction with ethyl acetate. The calibration curves were linear in the range of 1.6-20000 ng/mL for four coumarins with the lower limit of quantitation of 1.6 ng/mL in rat tissues. The intraday and interday precisions and recoveries were all within 80-100% with the relative standard deviations (RSDs) which were all less than 10.9%. The method was successfully applied to the tissue distribution research after oral administration of 6.0 g/kg APR extract to rat. The results revealed that the tissues distributions of four coumarins were in the liver, followed by the ovary, uterus, kidney, lung, heart, spleen, and muscle.

scholarly journals The distinction between γ-glutamylhydroxamate synthetase and l-glutamine–hydroxylamine glutamyltransferase activities in rat tissues. Studies in vivo

1973 ◽  
Vol 133 (1) ◽  
pp. 59-66 ◽  
Author(s):  
Annemarie Herzfeld ◽  
Nathan A. Estes
Keyword(s):  
Tissue Distribution ◽  
Enzyme Activities ◽  
Differential Response ◽  
Rat Tissues ◽  
Synthetase Activity ◽  
Assay Condition ◽  
Foetal Liver ◽  
Males And Females ◽  
Neonatal Kidney

The formation of γ-glutamylhydroxamate by homogenates under optimum assay condition showed an inconstancy in the ratios of the enzyme activities utilizing l-glutamate and ATP (γ-glutamylhydroxamate synthetase) and l-glutamine and ADP (l-glutamine–hydroxylamine glutamyltransferase) in a number of normal and neoplastic rat tissues. Although γ-glutamylhydroxamate synthetase activities in adult livers and kidneys were identical in males and females, l-glutamine–hydroxylamine glutamyltransferase activities in the organs of females were significantly lower. The developmental formations of the two activities in liver, kidney, brain and muscle were not simultaneous. The l-glutamine–hydroxylamine glutamyltransferase activity in foetal liver or neonatal kidney could be prematurely evoked by thyroxine, but the γ-glutamylhydroxamate synthetase activity remained unchanged. Injections of cortisol also had dissimilar effects on the two activities in thymus and hepatomas. The discrepant tissue distribution, asynchronous developmental formation and differential response to several hormonal stimuli provide evidence in vivo that the two activities are not catalysed by the same protein.

scholarly journals Localization of EGF receptors in frozen tissue sections by antibody and biotinylated EGF-based techniques.

1994 ◽  
Vol 42 (3) ◽  
pp. 307-314 ◽  
Author(s):  
J R Reeves ◽  
T G Cooke ◽  
D Fenton-Lee ◽  
A M McNicol ◽  
B W Ozanne ◽  
...  
Keyword(s):  
Egf Receptor ◽  
Detection Method ◽  
Rat Tissues ◽  
Positive Staining ◽  
Egf Receptors ◽  
Immunohistochemical Technique ◽  
Tissue Sections ◽  
Frozen Tissue ◽  
Normal Human ◽  
Receptor Monoclonal Antibody

We developed a sensitive EGF receptor detection method for frozen tissue sections using biotinylated EGF as the primary reagent. The method was directly compared with an immunohistochemical technique based on an anti-EGF receptor monoclonal antibody (MAb EGFR1) in normal human and rat tissues and in human tumors. The method was more sensitive than a previously published biotinylated EGF-based technique. In normal human tissues and in 37 of the 50 tumors, the binding pattern mirrored that of positive staining with EGFR1. Five further tumors showed weak immunoreactivity, but in these no binding of biotinylated EGF was detected. The remaining eight tumors were negative by both techniques. The discordant cases may reflect a lower level of sensitivity of the ligand-binding technique or, alternatively, abnormal receptors may have been expressed in these tissues. EGF receptors could be detected in rat liver with biotinylated EGF but not with the antibody, indicating the usefulness of the ligand-based technique in cross-species studies.

scholarly journals Rapid brain structure and tumour margin detection on whole frozen tissue sections by fast multiphotometric mid-infrared scanning

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Tim Kümmel ◽  
Björn van Marwick ◽  
Miriam Rittel ◽  
Carina Ramallo Guevara ◽  
Felix Wühler ◽  
...  
Keyword(s):  
Imaging System ◽  
Frozen Section ◽  
Computational Effort ◽  
Primary Hepatocellular Carcinoma ◽  
Measuring System ◽  
Sufficient Information ◽  
Tissue Samples ◽  
Mid Infrared ◽  
Tissue Sections ◽  
Frozen Tissue

AbstractFrozen section analysis is a frequently used method for examination of tissue samples, especially for tumour detection. In the majority of cases, the aim is to identify characteristic tissue morphologies or tumour margins. Depending on the type of tissue, a high number of misdiagnoses are associated with this process. In this work, a fast spectroscopic measurement device and workflow was developed that significantly improves the speed of whole frozen tissue section analyses and provides sufficient information to visualize tissue structures and tumour margins, dependent on their lipid and protein molecular vibrations. That optical and non-destructive method is based on selected wavenumbers in the mid-infrared (MIR) range. We present a measuring system that substantially outperforms a commercially available Fourier Transform Infrared (FT-IR) Imaging system, since it enables acquisition of reduced spectral information at a scan field of 1 cm2 in 3 s, with a spatial resolution of 20 µm. This allows fast visualization of segmented structure areas with little computational effort. For the first time, this multiphotometric MIR system is applied to biomedical tissue sections. We are referencing our novel MIR scanner on cryopreserved murine sagittal and coronal brain sections, especially focusing on the hippocampus, and show its usability for rapid identification of primary hepatocellular carcinoma (HCC) in mouse liver.

scholarly journals Biofilm formation in the lung contributes to virulence and drug tolerance of Mycobacterium tuberculosis

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Poushali Chakraborty ◽  
Sapna Bajeli ◽  
Deepak Kaushal ◽  
Bishan Dass Radotra ◽  
Ashwani Kumar
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Immune System ◽  
Biofilm Formation ◽  
Antimycobacterial Activity ◽  
Drug Tolerance ◽  
Host Immune System ◽  
Tissue Sections ◽  
Slow Growing

AbstractTuberculosis is a chronic disease that displays several features commonly associated with biofilm-associated infections: immune system evasion, antibiotic treatment failures, and recurrence of infection. However, although Mycobacterium tuberculosis (Mtb) can form cellulose-containing biofilms in vitro, it remains unclear whether biofilms are formed during infection in vivo. Here, we demonstrate the formation of Mtb biofilms in animal models of infection and in patients, and that biofilm formation can contribute to drug tolerance. First, we show that cellulose is also a structural component of the extracellular matrix of in vitro biofilms of fast and slow-growing nontuberculous mycobacteria. Then, we use cellulose as a biomarker to detect Mtb biofilms in the lungs of experimentally infected mice and non-human primates, as well as in lung tissue sections obtained from patients with tuberculosis. Mtb strains defective in biofilm formation are attenuated for survival in mice, suggesting that biofilms protect bacilli from the host immune system. Furthermore, the administration of nebulized cellulase enhances the antimycobacterial activity of isoniazid and rifampicin in infected mice, supporting a role for biofilms in phenotypic drug tolerance. Our findings thus indicate that Mtb biofilms are relevant to human tuberculosis.

IN VIVO INTERCONVERSION FACTORS BETWEEN OESTRONE AND 17 -OESTRADIOL IN RAT TISSUES ( TT)

1971 ◽  
Vol 66 (3) ◽  
pp. 417-430 ◽  
Author(s):  
R. D. Hertogh ◽  
E. Ekka ◽  
I. Vanderheyden
Keyword(s):  
Rat Tissues

Raman molecular imaging of brain frozen tissue sections

2014 ◽  
Vol 120 (1) ◽  
pp. 55-62 ◽  
Author(s):  
Rachel E. Kast ◽  
Gregory W. Auner ◽  
Mark L. Rosenblum ◽  
Tom Mikkelsen ◽  
Sally M. Yurgelevic ◽  
...  
Keyword(s):  
Molecular Imaging ◽  
Tissue Sections ◽  
Frozen Tissue
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