scholarly journals The distribution of acid and alkaline ribonuclease activities in preneoplastic and neoplastic rat livers.

1977 ◽  
Vol 25 (2) ◽  
pp. 115-121 ◽  
Author(s):  
S M Murthy ◽  
R Daoust
Keyword(s):  
Ribonucleic Acid ◽  
Serial Sections ◽  
Parenchymal Tissue ◽  
Precipitation Technique ◽  
Stromal Tissue ◽  
Alkaline Ribonuclease ◽  
Rat Livers ◽  
Film Method ◽  
Substrate Film

Ribonuclease (RNase) activities revealed by the substrate film method were compared with reactions for acid and alkaline RNase obtained by lead precipitation technique in serial sections of preneoplastic livers and hepatomas. The preneoplastic parenchymal tissue giving positive reactions with ribonucleic acid films showed both acid and alkaline RNase activities by lead precipitation technique, and the area of hyperplastic nodules nonreactive against substrate films were deficient in acid and alkaline RNase activities. Preneoplastic hyperbasophilic foci and hepatoma gave weak or negative reactions by either method, but necrotic areas and stromal tissue showed appreciable RNase activities. Thus a good correlation was observed in these tissues between the RNase activities revealed by the film method and those demonstrated by lead precipitation.

In Vivo Effect of Heparin on Acid and Alkaline Ribonuclease Activities of Mouse Liver

1956 ◽  
Vol 184 (2) ◽  
pp. 415-417 ◽  
Author(s):  
Gaston De Lamirande ◽  
George Weber ◽  
Antonio Cantero
Keyword(s):  
Tissue Culture ◽  
Body Weight ◽  
Single Dose ◽  
Ribonucleic Acid ◽  
Mouse Liver ◽  
Single Injection ◽  
Coagulation Time ◽  
Alkaline Ribonuclease ◽  
Blood Coagulability

A single dose of 30 µg/gm body weight of depo-heparin was injected subcutaneously into white Swiss mice. At 1, 3, 6 and 12 hours after the injection, the blood coagulation time was measured and the activity of acid and alkaline ribonuclease of liver was determined. This single injection of depo-heparin significantly inhibited the acid and alkaline ribonucleases of liver 1 hour after injection. The enzymatic activities significantly increased after the blood coagulability was restored. The in vivo inhibition of acid and alkaline ribonuclease activity supports the explanation that the accumulation of ribonucleic acid in cells of tissue culture in the presence of heparin might be due to the inhibition of ribonuclease.

scholarly journals Cytochemical detection of hyaluronidase activity in single human and mouse sperm by an improved substrate-film technique.

1984 ◽  
Vol 32 (1) ◽  
pp. 63-66 ◽  
Author(s):  
R Waibel ◽  
L C Ginsberg ◽  
G Ficsor
Keyword(s):  
Germ Cells ◽  
Screening Method ◽  
Human Sperm ◽  
Simple Method ◽  
Male Germ Cells ◽  
Mouse Sperm ◽  
Hyaluronidase Activity ◽  
Film Method ◽  
Human And Mouse ◽  
Substrate Film

A substrate-film method is described that allows the detection of hyaluronidase activity in nearly 100% of single human and mouse sperm. The level of hyaluronidase activity as determined by halo diameters was greater in mouse than in human sperm. This simple method may have use as a screening method for identifying compounds that cause developmental or genetic defects in male germ cells, or for the diagnosis of infertility due to decreased hyaluronidase activity.

scholarly journals LOCALIZATION AND ONSET OF AMYLASE ACTIVITY IN MOUSE SALIVARY GLANDS DETERMINED BY A SUBSTRATE FILM METHOD

1971 ◽  
Vol 19 (5) ◽  
pp. 310-319 ◽  
Author(s):  
RICHARD J. SMITH ◽  
JACK FROMMER ◽  
ROBERT SCHIFF
Keyword(s):  
Parotid Gland ◽  
Salivary Glands ◽  
Amylase Activity ◽  
Submandibular Glands ◽  
Males And Females ◽  
Starch Substrate ◽  
Convoluted Tubules ◽  
Film Method ◽  
Positive Results ◽  
Substrate Film

A starch substrate film technique was used to determine the localization and onset of amylase activity in the major salivary glands of mice. The earliest activity occurred in the parotid gland of males and females at 8 days of age. The intensity of the reaction rapidly increased so that by day 16 the entire parotid gland was amylase-positive. The submandibular gland exhibited the sexual dimorphism that has been characterized for the adult gland. Amylase activity was observed in the male at 24 days of age, shortly after the first morphologic differences between the submandibular glands of males and females became apparent. The female showed positive results at 36 days of age. Amylase activity in the submandibular glands was localized to the convoluted tubules. As the gland developed, the amylase activity increased in correlation with the increasing concentration of these tubules. The sublingual gland was always amylase-negative.

scholarly journals THE DISTRIBUTION OF DEOXYRIBONUCLEASE IN NORMAL, CIRRHOTIC AND NEOPLASTIC RAT LIVERS

1959 ◽  
Vol 7 (2) ◽  
pp. 139-143 ◽  
Author(s):  
R. DAOUST ◽  
A. CANTERO
Keyword(s):  
Enzyme Activity ◽  
Bile Duct ◽  
Normal Liver ◽  
Liver Parenchyma ◽  
Cirrhotic Liver ◽  
The Other ◽  
Parenchymal Cells ◽  
Rat Livers ◽  
Film Method ◽  
Dna Film

The distribution of deoxyribonuclease in normal, cirrhotic and neoplastic rat livers was investigated histochemically using the gelatine-DNA film method. The bile duct cells and connective tissue elements which are present in abnormal amounts in cirrhotic liver contain little DNAase activity compared with parenchymal tissue. The distribution pattern of DNAase is relatively uniform in normal liver parenchyma but becomes heterogeneous in the parenchyma of cirrhotic and neoplastic tissues. Groups of parenchymal cells in cirrhotic liver and the hepatoma cells in general appear devoid of DNAase activity. The necrotic areas of tissues, on the other hand, show intense enzyme activity.

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