A SIMPLE METHOD FOR THE DETERMINATION OF WATER CONTENT AND PROTEIN CONCENTRATION IN SUBCELLULAR ELEMENTS

U. GLAS; G. F. BAHR

doi:10.1177/14.1.40

A SIMPLE METHOD FOR THE DETERMINATION OF WATER CONTENT AND PROTEIN CONCENTRATION IN SUBCELLULAR ELEMENTS

Journal of Histochemistry & Cytochemistry ◽

10.1177/14.1.40 ◽

1966 ◽

Vol 14 (1) ◽

pp. 40-44 ◽

Cited By ~ 3

Author(s):

U. GLAS ◽

G. F. BAHR

Keyword(s):

Nitrogen Content ◽

Water Content ◽

Protein Concentration ◽

Liver Mitochondrion ◽

Accurate Method ◽

Absorption Tube ◽

Simple Method ◽

Gravimetric Determination ◽

Average Water

An accurate method for the determination of the average water content of subcellular particles from their suspensions is presented. The particle is freed from water by spinning it from its suspension medium into oil of high density. In the oil it is broken up, and the water is distilled off for its gravimetric determination in an absorption tube. The dry particle remnants are further centrifuged back into a watery medium in which the nitrogen content is determined A. protein content of 26.3% for the average rat liver mitochondrion is found.

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Total body water in Merino and Romney Marsh Sheep estimated by alcohol (ethanol) dilution.

Australian Journal of Agricultural Research ◽

10.1071/ar9610681 ◽

1961 ◽

Vol 12 (4) ◽

pp. 681 ◽

Cited By ~ 7

Author(s):

OE Budtz-Olsen ◽

JD Cleeve ◽

BA Oelrichs

Keyword(s):

Water Content ◽

Total Body Water ◽

Fat Content ◽

Body Water ◽

Simple Method ◽

Total Body

A simple method for the estimation of total body water in sheep by alcohol dilution is described. It was found that Romney Marsh ewes have a significantly higher water content than Merinos. Increased total body water is therefore not the factor which allows the Merino to survive in a hot dry environment in which the Romney Marsh does not thrive. It is pointed out that total body water cannot be used in the usual way for the determination of the fat content in live sheep.

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Characteristic of Allergen Protein of Nila Baby Fish (Oreochromis niloticus) and Its Processed Product

Jurnal Kimia VALENSI ◽

10.15408/jkv.v6i1.12885 ◽

2020 ◽

Vol 6 (1) ◽

pp. 32-39

Author(s):

Hendra Wijaya ◽

Sri Yadial Chalid ◽

Ning Ima Arie Wardayanie ◽

Widyaningsih Widyaningsih ◽

Santi Ariningsih

Keyword(s):

Water Content ◽

Oreochromis Niloticus ◽

Protein Concentration ◽

Protein Band ◽

Specific Ige ◽

Proximate Analysis ◽

Fat Content ◽

Carbohydrate Content ◽

Allergen Protein

Allergen protein is a protein that could triggers allergy reaction. This study purposes to examine the characteristic of allergen protein of unripe, boiled, and fried nila baby fish. Characteristic of allergen protein were observed by electrophoresis and immunoblotting method, while the determination of protein concentration was observed by Bradford method. Identification of the used nila baby fish was accomplished in Faculty of Fishery and Marine Science of IPB. The result of identification showed that the used nila baby fish was Oreochromis niloticus. The proximate analysis of unripe, boiled, and fried nila baby fish resulted water content ranged from 19.16%-23.68%, fat content ranged from 1.03%-20.44% and carbohydrate content ranged from 0.16%-20.27%. Protein concentration of unripe, boiled, and fried nila baby fish extract respectively were 1963.45 mg/L; 699.82 mg/L; 607.79 mg/L. The band amount of allergen protein of unripe, boiled, and fried nila baby fish which was detected by electrophoresis, respectively were 16 of protein band, 26 of protein band and 16 of protein band. The immunoblotting showed that the sum of respondent who contained specific IgE that can bind with allergen protein of boiled and fried nila baby fish were more than allergen protein of unripe nila baby fish. It indicated that the processing process by boiling and frying would increase allergenicity toward nila baby fish.

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