Total body water in Merino and Romney Marsh Sheep estimated by alcohol (ethanol) dilution.

1961 ◽  
Vol 12 (4) ◽  
pp. 681 ◽  
Author(s):  
OE Budtz-Olsen ◽  
JD Cleeve ◽  
BA Oelrichs
Keyword(s):  
Water Content ◽  
Total Body Water ◽  
Fat Content ◽  
Body Water ◽  
Simple Method ◽  
Total Body

A simple method for the estimation of total body water in sheep by alcohol dilution is described. It was found that Romney Marsh ewes have a significantly higher water content than Merinos. Increased total body water is therefore not the factor which allows the Merino to survive in a hot dry environment in which the Romney Marsh does not thrive. It is pointed out that total body water cannot be used in the usual way for the determination of the fat content in live sheep.

Determination of Body Water Content Using Trace Levels of Deuterium Oxide and Infrared Spectrophotometry

1968 ◽  
Vol 14 (11) ◽  
pp. 1112-1124 ◽  
Author(s):  
Marion J Stansell ◽  
Luis Mojica
Keyword(s):  
Water Content ◽  
Total Body Water ◽  
Human Subjects ◽  
Deuterium Oxide ◽  
Body Water ◽  
Infrared Spectrophotometry ◽  
Serum Samples ◽  
Vacuum Sublimation ◽  
Total Body

Abstract A procedure is described for the determination of total body water content of human subjects using 11- to 12-gm. doses of deuterium oxide (D2O), vacuum sublimation of serum samples, and quantitation of the deuterium by infrared spectrophotometry at 2510 cm.-1. The coefficient of variation based on day-to-day procedure reproducibility is less than 2%. D2O added to serum is completely recovered in the sublimation and assay operations. The D2O dilution procedure compares favorably with the tritium dilution technic, with values obtained on 45 out of 46 individuals falling within the ±3 combined S.D. limits for the two methods. The significance of the total body water data is discussed.

Estimation of water content by tritium dilution of animals subjected to rapid live-weight changes

1969 ◽  
Vol 72 (1) ◽  
pp. 31-40 ◽  
Author(s):  
W. R. McManus ◽  
R. K. Prichard ◽  
Carolyn Baker ◽  
M. V. Petruchenia
Keyword(s):  
Body Weight ◽  
Water Content ◽  
Total Body Water ◽  
Compensatory Growth ◽  
Specific Activity ◽  
Tritiated Water ◽  
Body Water ◽  
Under Nutrition ◽  
The Mean ◽  
Total Body

SUMMARYThe use of tritiated water to estimate total body-water content of animals experiencing recovery from under-nutrition was studied.The time for equilibration of tritiated water (TOH), given intraperitoneally, with total body water (TBW) was determined in rabbits and in rats. As judged by the specific activity of blood water, equilibration had occurred by 76–125 min in the rabbit and did not appear to be affected by the plane of nutrition. However, between slaughter groups the specific activity of water obtained from the liver 180 min after injection of TOH was significantly different from the specific activity of water simultaneously obtained from the blood plasma. It is concluded that the liver is not a suitable tissue to use for testing achievement of equilibration.As judged by the specific activity of blood water compared to that of water from the whole body macerate, equilibration in mature rats either in stable body condition or undergoing rapid compensatory growth occurred in less than 60 min.A trial comparing TOH-space (corrected by 3% body weight) and actual TBW (by desiccation) was conducted on thirty rabbits which experienced under-nutrition followed by compensatory growth.Prior to under-nutrition the agreement between actual and estimated TBW was satisfactory and within 2·3%. During compensatory growth the agreement was poor— the TOH values over-estimating actual TBW by about 12%.A trial with mature rats confirmed the findings with rabbits. For rats in stable body weight the mean estimated TOH-space for fourteen animals was within 1·2% of the actual TBW. For fourteen rats undergoing compensatory growth the mean estimated TOH-space (corrected by 3% body weight) overestimated actual TBW by 6·2%.

Determination of total body water in uraemic patients by bioelectrical impedance

1993 ◽  
Vol 8 (8) ◽  
pp. 716-719 ◽  
Author(s):  
C. H. Kong ◽  
C. M. Thompson ◽  
C. A. Lewis ◽  
P. D. Hill ◽  
F. D. Thompson
Keyword(s):  
Total Body Water ◽  
Bioelectrical Impedance ◽  
Body Water ◽  
Total Body

scholarly journals Determination of total body water by a simple and rapid mass spectrometric method

1996 ◽  
Vol 31 (1) ◽  
pp. 108-111 ◽  
Author(s):  
B. K. Van Kreel ◽  
F. Van der Vegt ◽  
M. Meers ◽  
T. Wagenmakers ◽  
K. Westerterp ◽  
...  
Keyword(s):  
Total Body Water ◽  
Mass Spectrometric ◽  
Body Water ◽  
Spectrometric Method ◽  
Mass Spectrometric Method ◽  
Total Body ◽  
Rapid Mass
Sign in / Sign up

Export Citation Format

Share Document