scholarly journals Glucose transport in cultured human skeletal muscle cells. Regulation by insulin and glucose in nondiabetic and non-insulin-dependent diabetes mellitus subjects.

1995 ◽  
Vol 96 (6) ◽  
pp. 2820-2827 ◽  
Author(s):  
T P Ciaraldi ◽  
L Abrams ◽  
S Nikoulina ◽  
S Mudaliar ◽  
R R Henry
Keyword(s):  
Diabetes Mellitus ◽  
Skeletal Muscle ◽  
Glucose Transport ◽  
Human Skeletal Muscle ◽  
Muscle Cells ◽  
Insulin Dependent Diabetes Mellitus ◽  
Skeletal Muscle Cells ◽  
Dependent Diabetes Mellitus ◽  
Human Skeletal Muscle Cells ◽  
Insulin Dependent

Glucose uptake and metabolism by cultured human skeletal muscle cells: rate-limiting steps

2001 ◽  
Vol 281 (1) ◽  
pp. E72-E80 ◽  
Author(s):  
Laureta M. Perriott ◽  
Tetsuro Kono ◽  
Richard R. Whitesell ◽  
Susan M. Knobel ◽  
David W. Piston ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Glucose Metabolism ◽  
Glucose Transport ◽  
Human Skeletal Muscle ◽  
Primary Cultures ◽  
Insulin Response ◽  
Muscle Cells ◽  
Skeletal Muscle Cells ◽  
Insulin Effect ◽  
Human Skeletal Muscle Cells

To use primary cultures of human skeletal muscle cells to establish defects in glucose metabolism that underlie clinical insulin resistance, it is necessary to define the rate-determining steps in glucose metabolism and to improve the insulin response attained in previous studies. We modified experimental conditions to achieve an insulin effect on 3- O-methylglucose transport that was more than twofold over basal. Glucose phosphorylation by hexokinase limits glucose metabolism in these cells, because the apparent Michaelis-Menten constant of coupled glucose transport and phosphorylation is intermediate between that of transport and that of the hexokinase and because rates of 2-deoxyglucose uptake and phosphorylation are less than those of glucose. The latter reflects a preference of hexokinase for glucose over 2-deoxyglucose. Cellular NAD(P)H autofluorescence, measured using two-photon excitation microscopy, is both sensitive to insulin and indicative of additional distal control steps in glucose metabolism. Whereas the predominant effect of insulin in human skeletal muscle cells is to enhance glucose transport, phosphorylation, and steps beyond, it also determines the overall rate of glucose metabolism.

scholarly journals ATP-Sensitive Potassium Channels Modulate Glucose Transport in Cultured Human Skeletal Muscle Cells.

2001 ◽  
Vol 48 (3) ◽  
pp. 369-375 ◽  
Author(s):  
TARO WASADA ◽  
TAKASHI YANO ◽  
MASAHIKO OHTA ◽  
NAOKO YUI ◽  
YASUHIKO IWAMOTO
Keyword(s):  
Skeletal Muscle ◽  
Potassium Channels ◽  
Glucose Transport ◽  
Human Skeletal Muscle ◽  
Muscle Cells ◽  
Skeletal Muscle Cells ◽  
Human Skeletal Muscle Cells
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