scholarly journals ATP-Sensitive Potassium Channels Modulate Glucose Transport in Cultured Human Skeletal Muscle Cells.

2001 ◽  
Vol 48 (3) ◽  
pp. 369-375 ◽  
Author(s):  
TARO WASADA ◽  
TAKASHI YANO ◽  
MASAHIKO OHTA ◽  
NAOKO YUI ◽  
YASUHIKO IWAMOTO
Keyword(s):  
Skeletal Muscle ◽  
Potassium Channels ◽  
Glucose Transport ◽  
Human Skeletal Muscle ◽  
Muscle Cells ◽  
Skeletal Muscle Cells ◽  
Human Skeletal Muscle Cells

Glucose uptake and metabolism by cultured human skeletal muscle cells: rate-limiting steps

2001 ◽  
Vol 281 (1) ◽  
pp. E72-E80 ◽  
Author(s):  
Laureta M. Perriott ◽  
Tetsuro Kono ◽  
Richard R. Whitesell ◽  
Susan M. Knobel ◽  
David W. Piston ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Glucose Metabolism ◽  
Glucose Transport ◽  
Human Skeletal Muscle ◽  
Primary Cultures ◽  
Insulin Response ◽  
Muscle Cells ◽  
Skeletal Muscle Cells ◽  
Insulin Effect ◽  
Human Skeletal Muscle Cells

To use primary cultures of human skeletal muscle cells to establish defects in glucose metabolism that underlie clinical insulin resistance, it is necessary to define the rate-determining steps in glucose metabolism and to improve the insulin response attained in previous studies. We modified experimental conditions to achieve an insulin effect on 3- O-methylglucose transport that was more than twofold over basal. Glucose phosphorylation by hexokinase limits glucose metabolism in these cells, because the apparent Michaelis-Menten constant of coupled glucose transport and phosphorylation is intermediate between that of transport and that of the hexokinase and because rates of 2-deoxyglucose uptake and phosphorylation are less than those of glucose. The latter reflects a preference of hexokinase for glucose over 2-deoxyglucose. Cellular NAD(P)H autofluorescence, measured using two-photon excitation microscopy, is both sensitive to insulin and indicative of additional distal control steps in glucose metabolism. Whereas the predominant effect of insulin in human skeletal muscle cells is to enhance glucose transport, phosphorylation, and steps beyond, it also determines the overall rate of glucose metabolism.

TGFß regulates metabolism of human skeletal muscle cells by miRNAs

2018 ◽  
Author(s):  
S Höckele ◽  
P Huypens ◽  
C Hoffmann ◽  
T Jeske ◽  
M Hastreiter ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Human Skeletal Muscle ◽  
Muscle Cells ◽  
Skeletal Muscle Cells ◽  
Human Skeletal Muscle Cells

Telomerase Alone Extends the Replicative Life Span of Human Skeletal Muscle Cells Without Compromising Genomic Stability

2003 ◽  
Vol 14 (15) ◽  
pp. 1473-1487 ◽  
Author(s):  
Martha Wootton ◽  
Karen Steeghs ◽  
Diana Watt ◽  
June Munro ◽  
Katrina Gordon ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Life Span ◽  
Human Skeletal Muscle ◽  
Genomic Stability ◽  
Muscle Cells ◽  
Skeletal Muscle Cells ◽  
Replicative Life Span ◽  
Human Skeletal Muscle Cells

scholarly journals Metabolomic analysis of primary human skeletal muscle cells during myogenic progression

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Ashok Kumar ◽  
Yashwant Kumar ◽  
Jayesh Kumar Sevak ◽  
Sonu Kumar ◽  
Niraj Kumar ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Human Skeletal Muscle ◽  
Muscle Cells ◽  
Skeletal Muscle Cells ◽  
Metabolomic Analysis ◽  
Human Skeletal Muscle Cells
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