scholarly journals Light-Induced Thickening of Photoreceptor Outer Segment Layer Detected by Ultra-High Resolution OCT Imaging

2016 ◽  
Vol 57 (9) ◽  
pp. OCT105 ◽  
Author(s):  
Yichao Li ◽  
Robert N. Fariss ◽  
Jennifer W. Qian ◽  
Ethan D. Cohen ◽  
Haohua Qian
Keyword(s):  
High Resolution ◽  
Outer Segment ◽  
Photoreceptor Outer Segment ◽  
Oct Imaging ◽  
High Resolution Oct

High-speed high-resolution OCT imaging of the retina with frequency swept lasers at 850 nm

2007 ◽  
Author(s):  
V. J. Srinivasan ◽  
R. Huber ◽  
I. Gorczynska ◽  
D. Adler ◽  
J. Y. Jiang ◽  
...  
Keyword(s):  
High Resolution ◽  
High Speed ◽  
Oct Imaging ◽  
High Resolution Oct

scholarly journals Three-dimensional high resolution OCT imaging of macular pathology

2009 ◽  
Vol 17 (5) ◽  
pp. 4037 ◽  
Author(s):  
C. Ahlers ◽  
U. Schmidt-Erfurth
Keyword(s):  
High Resolution ◽  
Three Dimensional ◽  
Oct Imaging ◽  
High Resolution Oct

scholarly journals Macular Pigment Optical Density and Photoreceptor Outer Segment Length as Predisease Biomarkers for Age-Related Macular Degeneration

2020 ◽  
Vol 9 (5) ◽  
pp. 1347 ◽  
Author(s):  
Norihiro Nagai ◽  
Sakiko Minami ◽  
Misa Suzuki ◽  
Hajime Shinoda ◽  
Toshihide Kurihara ◽  
...  
Keyword(s):  
Macular Degeneration ◽  
Optical Density ◽  
Outer Segment ◽  
Macular Pigment ◽  
Age Related Macular Degeneration ◽  
Segment Length ◽  
Macular Pigment Optical Density ◽  
Photoreceptor Outer Segment ◽  
Age Related ◽  
Fellow Eyes

To explore predisease biomarkers, which may help screen for the risk of age-related macular degeneration (AMD) at very early stages, macular pigment optical density (MPOD) and photoreceptor outer segment (PROS) length were analyzed. Thirty late AMD fellow eyes, which are at high risk and represent the predisease condition of AMD, were evaluated and compared with 30 age-matched control eyes without retinal diseases; there was no early AMD involvement in the AMD fellow eyes. MPOD was measured using MPS2® (M.E. Technica Co. Ltd., Tokyo, Japan), and PROS length was measured based on optical coherence tomography images. MPOD levels and PROS length in the AMD fellow eyes were significantly lower and shorter, respectively, than in control eyes. MPOD and PROS length were positively correlated in control eyes (R = 0.386; p = 0.035) but not in AMD fellow eyes. Twenty (67%) AMD fellow eyes met the criteria of MPOD < 0.65 and/or PROS length < 35 μm, while only five (17%) control eyes did. After adjusting for age and sex, AMD fellow eyes more frequently satisfied the definition (p < 0.001; 95% confidence interval, 3.50–60.4; odds ratio, 14.6). The combination of MPOD and PROS length may be a useful biomarker for screening predisease AMD patients, although further studies are required in this regard.

Reliability Assessment of a Rod Photoreceptor Outer Segment Grading System

2001 ◽  
Vol 72 (5) ◽  
pp. 573-579 ◽  
Author(s):  
Monica M Jablonski ◽  
Marshall J Graney ◽  
Stephen B Kritchevsky ◽  
Alessandro Iannaccone
Keyword(s):  
Outer Segment ◽  
Reliability Assessment ◽  
Grading System ◽  
Rod Photoreceptor ◽  
Photoreceptor Outer Segment

scholarly journals Correlation between brain volume and retinal photoreceptor outer segment volume in normal aging and neurodegenerative diseases

PLoS ONE ◽  
2020 ◽  
Vol 15 (9) ◽  
pp. e0237078
Author(s):  
Atsuro Uchida ◽  
Jagan A. Pillai ◽  
Robert Bermel ◽  
Stephen E. Jones ◽  
Hubert Fernandez ◽  
...  
Keyword(s):  
Neurodegenerative Diseases ◽  
Outer Segment ◽  
Brain Volume ◽  
Normal Aging ◽  
Photoreceptor Outer Segment ◽  
Retinal Photoreceptor ◽  
Segment Volume

scholarly journals TULP1 and TUB Are Required for Specific Localization of PRCD to Photoreceptor Outer Segments

2020 ◽  
Vol 21 (22) ◽  
pp. 8677
Author(s):  
Lital Remez ◽  
Ben Cohen ◽  
Mariela J. Nevet ◽  
Leah Rizel ◽  
Tamar Ben-Yosef
Keyword(s):  
Protein Interactions ◽  
Mammalian Cells ◽  
Outer Segment ◽  
Protein Protein Interactions ◽  
Photoreceptor Outer Segment ◽  
Photoreceptor Outer Segments ◽  
Outer Segments ◽  
Yeast Two Hybrid System ◽  
Specific Localization ◽  
Recruitment System

Photoreceptor disc component (PRCD) is a small protein which is exclusively localized to photoreceptor outer segments, and is involved in the formation of photoreceptor outer segment discs. Mutations in PRCD are associated with retinal degeneration in humans, mice, and dogs. The purpose of this work was to identify PRCD-binding proteins in the retina. PRCD protein-protein interactions were identified when implementing the Ras recruitment system (RRS), a cytoplasmic-based yeast two-hybrid system, on a bovine retina cDNA library. An interaction between PRCD and tubby-like protein 1 (TULP1) was identified. Co-immunoprecipitation in transfected mammalian cells confirmed that PRCD interacts with TULP1, as well as with its homolog, TUB. These interactions were mediated by TULP1 and TUB highly conserved C-terminal tubby domain. PRCD localization was altered in the retinas of TULP1- and TUB-deficient mice. These results show that TULP1 and TUB, which are involved in the vesicular trafficking of several photoreceptor proteins from the inner segment to the outer segment, are also required for PRCD exclusive localization to photoreceptor outer segment discs.

scholarly journals Photosensitized Oxidative Stress to ARPE-19 Cells Decreases Protein Receptors that Mediate Photoreceptor Outer Segment Phagocytosis

2013 ◽  
Vol 54 (3) ◽  
pp. 2276 ◽  
Author(s):  
Magdalena M. Olchawa ◽  
Anja M. Herrnreiter ◽  
Christine M. B. Skumatz ◽  
Mariusz Zareba ◽  
Tadeusz J. Sarna ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Outer Segment ◽  
Photoreceptor Outer Segment ◽  
Protein Receptors

scholarly journals High-Resolution Quantitative Mapping of Macaque Cervicovaginal Epithelial Thickness: Implications for Mucosal Vaccine Delivery

2021 ◽  
Vol 12 ◽  
Author(s):  
Kathleen L. Vincent ◽  
Patrice A. Frost ◽  
Massoud Motamedi ◽  
Edward J. Dick ◽  
Jingna Wei ◽  
...  
Keyword(s):  
High Resolution ◽  
Immune Responses ◽  
Real Time ◽  
Basal Layer ◽  
Mucosal Vaccine ◽  
P Value ◽  
Mechanical Abrasion ◽  
Mucosal Vaccination ◽  
Epithelial Thickness ◽  
Oct Imaging

Vaginal mucosal surfaces naturally offer some protection against sexually transmitted infections (STIs) including Human Immunodeficiency Virus-1, however topical preventative medications or vaccine designed to boost local immune responses can further enhance this protection. We previously developed a novel mucosal vaccine strategy using viral vectors integrated into mouse dermal epithelium to induce virus-specific humoral and cellular immune responses at the site of exposure. Since vaccine integration occurs at the site of cell replication (basal layer 100-400 micrometers below the surface), temporal epithelial thinning during vaccine application, confirmed with high resolution imaging, is desirable. In this study, strategies for vaginal mucosal thinning were evaluated noninvasively using optical coherence tomography (OCT) to map reproductive tract epithelial thickness (ET) in macaques to optimize basal layer access in preparation for future effective intravaginal mucosal vaccination studies. Twelve adolescent female rhesus macaques (5-7kg) were randomly assigned to interventions to induce vaginal mucosal thinning, including cytobrush mechanical abrasion, the chemical surfactant spermicide nonoxynol-9 (N9), the hormonal contraceptive depomedroxyprogesterone acetate (DMPA), or no intervention. Macaques were evaluated at baseline and after interventions using colposcopy, vaginal biopsies, and OCT imaging, which allowed for real-time in vivo visualization and measurement of ET of the mid-vagina, fornices, and cervix. P value ≤0.05 was considered significant. Colposcopy findings included pink, rugated tissue with variable degrees of white-tipped, thickened epithelium. Baseline ET of the fornices was thinner than the cervix and vagina (p&lt;0.05), and mensing macaques had thinner ET at all sites (p&lt;0.001). ET was decreased 1 month after DMPA (p&lt;0.05) in all sites, immediately after mechanical abrasion (p&lt;0.05) in the fornix and cervix, and after two doses of 4% N9 (1.25ml) applied over 14 hrs in the fornix only (p&lt;0.001). Histological assessment of biopsied samples confirmed OCT findings. In summary, OCT imaging allowed for real time assessment of macaque vaginal ET. While varying degrees of thinning were observed after the interventions, limitations with each were noted. ET decreased naturally during menses, which may provide an ideal opportunity for accessing the targeted vaginal mucosal basal layers to achieve the optimum epithelial thickness for intravaginal mucosal vaccination.

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