scholarly journals Perturbation of Hyaluronan Synthesis in the Trabecular Meshwork and the Effects on Outflow Facility

2012 ◽  
Vol 53 (8) ◽  
pp. 4616 ◽  
Author(s):  
Kate E. Keller ◽  
Ying Ying Sun ◽  
Yong-Feng Yang ◽  
John M. Bradley ◽  
Ted S. Acott
Keyword(s):  
Trabecular Meshwork ◽  
Outflow Facility

scholarly journals Prostaglandins Increase Trabecular Meshwork Outflow Facility in Cultured Human Anterior Segments

2008 ◽  
Vol 145 (1) ◽  
pp. 114-119 ◽  
Author(s):  
Cindy K. Bahler ◽  
Kyle G. Howell ◽  
Cheryl R. Hann ◽  
Michael P. Fautsch ◽  
Douglas H. Johnson
Keyword(s):  
Trabecular Meshwork ◽  
Outflow Facility ◽  
Anterior Segments

Differential Effects of Trabecular Meshwork Stiffness on Outflow Facility in Normal Human and Porcine Eyes

2012 ◽  
Vol 53 (9) ◽  
pp. 5242 ◽  
Author(s):  
Lucinda J. Camras ◽  
W. Daniel Stamer ◽  
David Epstein ◽  
Pedro Gonzalez ◽  
Fan Yuan
Keyword(s):  
Trabecular Meshwork ◽  
Outflow Facility ◽  
Differential Effects ◽  
Normal Human ◽  
Porcine Eyes

scholarly journals The role of calcium-independent phospholipase A2γ in modulation of aqueous humor drainage and Ca2+ sensitization of trabecular meshwork contraction

2012 ◽  
Vol 302 (7) ◽  
pp. C979-C991 ◽  
Author(s):  
Padmanabhan P. Pattabiraman ◽  
Fred B. Lih ◽  
Kenneth B. Tomer ◽  
Ponugoti Vasantha Rao
Keyword(s):  
Arachidonic Acid ◽  
Aqueous Humor ◽  
Trabecular Meshwork ◽  
Rho Gtpase ◽  
Focal Adhesions ◽  
Intense Staining ◽  
Outflow Facility ◽  
Bromoenol Lactone ◽  
Mlc Phosphorylation

The contractile and relaxation characteristics of trabecular meshwork (TM) are presumed to influence aqueous humor (AH) drainage and intraocular pressure. The mechanisms underlying regulation of TM cell contractile properties, however, are not well understood. This study investigates the role of calcium-independent phospholipase A2 (iPLA2), which controls eicosanoid synthesis, in regulation of TM cell contraction and AH outflow using mechanism-based isoform specific inhibitors (R)-bromoenol lactone (R-BEL, iPLA2γ specific) and (S)-bromoenol lactone (S-BEL, iPLA2β specific). Immunohistochemical analysis revealed intense staining for both iPLA2β and γ isoforms throughout the TM, juxtacanalicular tissue, and Schlemm's canal of human eye. Inhibition of iPLA2γ by R-BEL or small interfering RNA-mediated silencing of iPLA2γ expression induced dramatic changes in TM cell morphology, and decreased actin stress fibers, focal adhesions, and myosin light-chain (MLC) phosphorylation. AH outflow facility increased progressively and significantly in enucleated porcine eyes perfused with R-BEL. This response was associated with a significant decrease in TM tissue MLC phosphorylation and alterations in the morphology of aqueous plexi in R-BEL-perfused eyes. In contrast, S-BEL did not affect either of these parameters. Additionally, R-BEL-induced cellular relaxation of the TM was associated with a significant decrease in the levels of active Rho GTPase, phospho-MLC phosphatase, phospho-CPI-17, and arachidonic acid. Taken together, these observations demonstrate that iPLA2γ plays a significant and isoform-specific role in regulation of AH outflow facility by altering the contractile characteristics of the TM. The effects of iPLA2γ on TM contractile status appear to involve arachidonic acid and Rho GTPase signaling pathways.

Comparing Viscocanalostomy with Trabeculectomy

2011 ◽  
Vol 04 (02) ◽  
pp. 88 ◽  
Author(s):  
Roberto G Carassa ◽  
Keyword(s):  
Intraocular Pressure ◽  
High Risk ◽  
Trabecular Meshwork ◽  
Meta Analysis ◽  
Effective Procedure ◽  
Open Angle ◽  
Outflow Facility ◽  
Morphologic Changes

Viscocanalostomy is a non-penetrating procedure aimed at increasing the outflow facility by creating significant morphologic changes at the trabecular meshwork level. It is an effective procedure in lowering intraocular pressure (IOP) in open-angle glaucomatous eyes. Compared with trabeculectomy, meta-analysis studies have shown that final IOPs are significantly higher. Nevertheless, viscocanalostomy is a safer technique with significantly fewer complications compared with trabeculectomy. Viscocanalostomy is therefore a better option in eyes where target IOPs are in the mid–high teens, or in eyes at high risk of complications with trabeculectomy.

scholarly journals The Structure of the Trabecular Meshwork, Its Connections to the Ciliary Muscle, and the Effect of Pilocarpine on Outflow Facility in Mice

2014 ◽  
Vol 55 (6) ◽  
pp. 3727 ◽  
Author(s):  
Darryl R. Overby ◽  
Jacques Bertrand ◽  
Martin Schicht ◽  
Friedrich Paulsen ◽  
W. Daniel Stamer ◽  
...  
Keyword(s):  
Trabecular Meshwork ◽  
Ciliary Muscle ◽  
Outflow Facility

Mathematical Modeling of Outflow Facility Increase With Trabecular Meshwork Bypass and Schlemm Canal Dilation

2016 ◽  
Vol 25 (4) ◽  
pp. 355-364 ◽  
Author(s):  
Fan Yuan ◽  
Andrew T. Schieber ◽  
Lucinda J. Camras ◽  
Paul J. Harasymowycz ◽  
Leon W. Herndon ◽  
...  
Keyword(s):  
Mathematical Modeling ◽  
Trabecular Meshwork ◽  
Outflow Facility
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