CB2 Cannabinoid Receptors in Trabecular Meshwork Cells Mediate JWH015-Induced Enhancement of Aqueous Humor Outflow Facility

2005 ◽  
Vol 46 (6) ◽  
pp. 1988 ◽  
Author(s):  
Lichun Zhong ◽  
Lijun Geng ◽  
YaFatou Njie ◽  
Wenke Feng ◽  
Zhao-Hui Song
Keyword(s):  
Aqueous Humor ◽  
Trabecular Meshwork ◽  
Cannabinoid Receptors ◽  
Outflow Facility ◽  
Aqueous Humor Outflow ◽  
Trabecular Meshwork Cells

Intracellular Cl regulates Na-K-Cl cotransport activity in human trabecular meshwork cells

1999 ◽  
Vol 277 (3) ◽  
pp. C373-C383 ◽  
Author(s):  
Luanna K. Putney ◽  
Cecile Rose T. Vibat ◽  
Martha E. O’Donnell
Keyword(s):  
Cell Volume ◽  
Aqueous Humor ◽  
Trabecular Meshwork ◽  
Cell Types ◽  
Cellular Mechanisms ◽  
Outflow Facility ◽  
Aqueous Humor Outflow ◽  
Trabecular Meshwork Cells ◽  
Cotransport System ◽  
Stimulation Of

The trabecular meshwork (TM) of the eye plays a central role in modulating intraocular pressure by regulating aqueous humor outflow, although the mechanisms are largely unknown. We and others have shown previously that aqueous humor outflow facility is modulated by conditions that alter TM cell volume. We have also shown that the Na-K-Cl cotransport system is a primary regulator of TM cell volume and that its activity appears to be coordinated with net efflux pathways to maintain steady-state volume. However, the cellular mechanisms that regulate cotransport activity and cell volume in TM cells have yet to be elucidated. The present study was conducted to investigate the hypothesis that intracellular Cl concentration ([Cl]i) acts to regulate TM cell Na-K-Cl cotransport activity, as has been shown previously for some other cell types. We demonstrate here that the human TM cell Na-K-Cl cotransporter is highly sensitive to changes in [Cl]i. Our findings reveal a marked stimulation of Na-K-Cl cotransport activity, assessed as ouabain-insensitive, bumetanide-sensitive K influx, in TM cells following preincubation of cells with Cl-free medium as a means of reducing [Cl]i. In contrast, preincubation of cells with media containing elevated K concentrations as a means of increasing [Cl]i results in inhibition of Na-K-Cl cotransport activity. The effects of reducing [Cl]i, as well as elevating [Cl]i, on Na-K-Cl cotransport activity are concentration dependent. Furthermore, the stimulatory effect of reduced [Cl]i is additive with cell-shrinkage-induced stimulation of the cotransporter. Our studies also show that TM cell Na-K-Cl cotransport activity is altered by a variety of Cl channel modulators, presumably through changes in [Cl]i. These findings support the hypothesis that regulation of Na-K-Cl cotransport activity, and thus cell volume, by [Cl]i may participate in modulating outflow facility across the TM.

scholarly journals Mechanisms of ATP release by human trabecular meshwork cells, the enabling step in purinergic regulation of aqueous humor outflow

2011 ◽  
Vol 227 (1) ◽  
pp. 172-182 ◽  
Author(s):  
Ang Li ◽  
Chi Ting Leung ◽  
Kim Peterson-Yantorno ◽  
W. Daniel Stamer ◽  
Claire H. Mitchell ◽  
...  
Keyword(s):  
Aqueous Humor ◽  
Trabecular Meshwork ◽  
Atp Release ◽  
Aqueous Humor Outflow ◽  
Trabecular Meshwork Cells

scholarly journals Role of MCP-1 and IL-8 in viral anterior uveitis, and contractility and fibrogenic activity of trabecular meshwork cells

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Jiyoung Lee ◽  
Jin A. Choi ◽  
Hyun-hee Ju ◽  
Ju-Eun Kim ◽  
Soon-Young Paik ◽  
...  
Keyword(s):  
Viral Infection ◽  
Aqueous Humor ◽  
Trabecular Meshwork ◽  
Anterior Uveitis ◽  
Rho Gtpase ◽  
Open Angle Glaucoma ◽  
Focal Adhesions ◽  
Aqueous Humor Outflow ◽  
Trabecular Meshwork Cells ◽  
Aqueous Outflow

AbstractThe inflammatory chemokines, monocyte chemoattractant protein (MCP)-1 and IL-8, are produced by normal trabecular meshwork cells (TM) and elevated in the aqueous humor of primary open angle glaucoma (POAG) and hypertensive anterior uveitis associated with viral infection. However, their role in TM cells and aqueous humor outflow remains unclear. Here, we explored the possible involvement of MCP-1 and IL-8 in the physiology of TM cells in the context of aqueous outflow, and the viral anterior uveitis. We found that the stimulation of human TM cells with MCP-1 and IL-8 induced significant increase in the formation of actin stress fibers and focal adhesions, myosin light chain phosphorylation, and the contraction of TM cells. MCP-1 and IL-8 also demonstrated elevation of extracellular matrix proteins, and the migration of TM cells. When TM cells were infected with HSV-1 and CMV virus, there was a significant increase in cytoskeletal contraction and Rho-GTPase activation. Viral infection of TM cells revealed significantly increased expression of MCP-1 and IL-8. Taken together, these results indicate that MCP-1 and IL-8 induce TM cell contractibility, fibrogenic activity, and plasticity, which are presumed to increase resistance to aqueous outflow in viral anterior uveitis and POAG.

scholarly journals New Insights Into Pathophysiological Mechanisms Regulating Conventional Aqueous Humor Outflow

Medicina ◽  
2013 ◽  
Vol 49 (4) ◽  
pp. 26 ◽  
Author(s):  
Daiva Paulavičiūtė-Baikštienė ◽  
Rūta Baršauskaitė ◽  
Ingrida Janulevičienė
Keyword(s):  
Intraocular Pressure ◽  
Aqueous Humor ◽  
Trabecular Meshwork ◽  
Kinase Inhibitors ◽  
Transforming Growth Factor ◽  
Protein Kinase Inhibitors ◽  
Pathophysiological Mechanisms ◽  
Aqueous Humor Outflow ◽  
Trabecular Meshwork Cells ◽  
Glaucoma Treatment

The aim of the article was to overview the pathophysiology of the conventional outflow pathway, trabecular meshwork, and intraocular pressure and to discuss the options of future glaucoma treatment directed to improvement in aqueous outflow. The literature search in the Medline, Embase, and Cochrane databases from April to May 2012 was performed; a total of 47 articles analyzed. The diminished conventional pathway may be altered by several pathophysiological mechanisms like TM obstruction caused by transforming growth factor-β2, clastic nondeformable cells, macrophages leaking from hypermature cataract, iris pigment, lens capsular fragments after YAG-laser posterior capsulotomy, proteins and their subfragments. It is known that trabecular meshwork contraction reduces outflow, and the actomyosin system is directly linked to this mechanism. New glaucoma drugs are still under investigation, but it is already proven that agents such as latranculin-B are effective in improving aqueous drainage. Selective Rho-associated coiled coilforming protein kinase inhibitors have been shown to cause a significant improvement in outflow facility and may become a new option for glaucoma treatment. Caldesmon negatively regulates actin-myosin interactions and thus increases outflow. Stem cells may replace missing or nonfunctional trabecular meshwork cells and hopefully will bring a new treatment solution. Pathophysiological mechanisms regulating conventional aqueous humor outflow are still not fully understood and require further investigations. Future treatment decisions should be directed to a specific mechanism regulating an elevation in intraocular pressure.

Noladin Ether Acts on Trabecular Meshwork Cannabinoid (CB1) Receptors to Enhance Aqueous Humor Outflow Facility

2006 ◽  
Vol 47 (5) ◽  
pp. 1999 ◽  
Author(s):  
Ya Fatou Njie ◽  
Akhilesh Kumar ◽  
Zhuanhong Qiao ◽  
Lichun Zhong ◽  
Zhao-Hui Song
Keyword(s):  
Aqueous Humor ◽  
Trabecular Meshwork ◽  
Cb1 Receptors ◽  
Outflow Facility ◽  
Aqueous Humor Outflow
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