scholarly journals Characterization of Ocular Surface Epithelial and Progenitor Cell Markers in Human Adipose Stromal Cells Derived from Lipoaspirates

2012 ◽  
Vol 53 (1) ◽  
pp. 513 ◽  
Author(s):  
Eva M. Martínez-Conesa ◽  
Enric Espel ◽  
Manuel Reina ◽  
Ricardo P. Casaroli-Marano
Keyword(s):  
Stromal Cells ◽  
Ocular Surface ◽  
Progenitor Cell ◽  
Adipose Stromal Cells ◽  
Cell Markers

Adipose Stromal Cells Stimulate Angiogenesis via Promoting Progenitor Cell Differentiation, Secretion of Angiogenic Factors, and Enhancing Vessel Maturation

2009 ◽  
Vol 15 (8) ◽  
pp. 2039-2050 ◽  
Author(s):  
Kseniya Rubina ◽  
Natalia Kalinina ◽  
Anastasia Efimenko ◽  
Tatyana Lopatina ◽  
Varvara Melikhova ◽  
...  
Keyword(s):  
Cell Differentiation ◽  
Stromal Cells ◽  
Progenitor Cell ◽  
Angiogenic Factors ◽  
Adipose Stromal Cells ◽  
Vessel Maturation

scholarly journals Mechanical Characterization of Extracellular Vesicles Derived from Immortalized Adipose Stromal Cells

2020 ◽  
Vol 118 (3) ◽  
pp. 252a
Author(s):  
Melissa C. Piontek ◽  
Sourav Maity ◽  
Linda A. Brouwer ◽  
Martin C. Harmsen ◽  
Wouter H. Roos
Keyword(s):  
Extracellular Vesicles ◽  
Stromal Cells ◽  
Mechanical Characterization ◽  
Adipose Stromal Cells

scholarly journals Proteoglycan synthesis in human and murine haematopoietic progenitor cell lines: isolation and characterization of a heparan sulphate proteoglycan as a major proteoglycan from the human haematopoietic cell line TF-1

1996 ◽  
Vol 317 (1) ◽  
pp. 203-212 ◽  
Author(s):  
Georg STÖCKER ◽  
Zofia DRZENIEK ◽  
Ursula JUST ◽  
Wolfram OSTERTAG ◽  
Barbara SIEBERTZ ◽  
...  
Keyword(s):  
Cell Line ◽  
Stromal Cells ◽  
Progenitor Cell ◽  
Heparan Sulphate ◽  
Proteoglycan Synthesis ◽  
Heparan Sulphate Proteoglycan ◽  
Haematopoietic Progenitor Cell ◽  
Sulphate Proteoglycan ◽  
Isolation And Characterization

Proteoglycans of bone-marrow stromal cells and their extracellular matrix are important components of the microenvironment of haematopoietic tissues. Proteoglycans might also be involved in the interaction of haematopoietic stem and stromal cells. Recently, several studies have been reported on the proteoglycan synthesis of stromal cells, but little is known about the proteoglycan synthesis of haematopoietic stem or progenitor cells. Here we report on the isolation and characterization of proteoglycans from two haematopoietic progenitor cell lines, the murine FDCP-Mix A4 and the human TF-1 cell line. Proteoglycans were isolated from metabolically labelled cells and purified by several chromatographic steps, including anion-exchange and size-exclusion chromatography. Biochemical characterization was performed by electrophoresis or gel-filtration chromatography before and after digestion with glycosaminoglycan-specific enzymes or HNO2 treatment. Whereas FDCP-Mix A4 cells synthesize a homogeneous chondroitin 4-sulphate proteoglycan, isolation and characterization of proteoglycans from the human cell line TF-1 revealed, that TF-1 cells synthesize, in addition to a chondroitin sulphate proteoglycan, a heparan sulphate proteoglycan as major proteoglycan. For this heparan sulphate proteoglycan a core protein size of approx. 59 kDa was determined. Immunochemical analysis of this heparan sulphate proteoglycan revealed that it is not related to the syndecan family nor to glypican.

scholarly journals Characterization of Cell Subpopulations Expressing Progenitor Cell Markers in Porcine Cardiac Valves

PLoS ONE ◽  
2013 ◽  
Vol 8 (7) ◽  
pp. e69667 ◽  
Author(s):  
Huan Wang ◽  
Balaji Sridhar ◽  
Leslie A. Leinwand ◽  
Kristi S. Anseth
Keyword(s):  
Progenitor Cell ◽  
Cardiac Valves ◽  
Cell Markers ◽  
Cell Subpopulations
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