Adipose Stromal Cells Stimulate Angiogenesis via Promoting Progenitor Cell Differentiation, Secretion of Angiogenic Factors, and Enhancing Vessel Maturation

2009 ◽  
Vol 15 (8) ◽  
pp. 2039-2050 ◽  
Author(s):  
Kseniya Rubina ◽  
Natalia Kalinina ◽  
Anastasia Efimenko ◽  
Tatyana Lopatina ◽  
Varvara Melikhova ◽  
...  
Keyword(s):  
Cell Differentiation ◽  
Stromal Cells ◽  
Progenitor Cell ◽  
Angiogenic Factors ◽  
Adipose Stromal Cells ◽  
Vessel Maturation

scholarly journals Biological and microbiological interactions of Ti-35Nb-7Zr alloy and its basic elements on bone marrow stromal cells: good prospects for bone tissue engineering

2020 ◽  
Vol 6 (1) ◽  
Author(s):  
Daphne de Camargo Reis Mello ◽  
Lais Morandini Rodrigues ◽  
Fabia Zampieri D’Antola Mello ◽  
Thais Fernanda Gonçalves ◽  
Bento Ferreira ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Cell Differentiation ◽  
Stromal Cells ◽  
Biofilm Formation ◽  
Bone Marrow Stromal Cells ◽  
Pure Titanium ◽  
Marrow Stromal Cells ◽  
The Other ◽  
Cell Based Therapy

Abstract Background An effective biomaterial for bone replacement should have properties to avoid bacterial contamination and promote bone formation while inducing rapid cell differentiation simultaneously. Bone marrow stem cells are currently being investigated because of their known potential for differentiation in osteoblast lineage. This makes these cells a good option for stem cell-based therapy. We have aimed to analyze, in vitro, the potential of pure titanium (Ti), Ti-35Nb-7Zr alloy (A), niobium (Nb), and zirconia (Zr) to avoid the microorganisms S. aureus (S.a) and P. aeruginosa (P.a). Furthermore, our objective was to evaluate if the basic elements of Ti-35Nb-7Zr alloy have any influence on bone marrow stromal cells, the source of stem cells, and observe if these metals have properties to induce cell differentiation into osteoblasts. Methods Bone marrow stromal cells (BMSC) were obtained from mice femurs and cultured in osteogenic media without dexamethasone as an external source of cell differentiation. The samples were divided into Ti-35Nb-7Zr alloy (A), pure titanium (Ti), Nb (niobium), and Zr (zirconia) and were characterized by scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDS). After predetermined periods, cell interaction, cytotoxicity, proliferation, and cell differentiation tests were performed. For monotypic biofilm formation, standardized suspensions (106 cells/ml) with the microorganisms S. aureus (S.a) and P. aeruginosa (P.a) were cultured for 24 h on the samples and submitted to an MTT test. Results All samples presented cell proliferation, growth, and spreading. All groups presented cell viability above 70%, but the alloy (A) showed better results, with statistical differences from Nb and Zr samples. Zr expressed higher ALP activity and was statistically different from the other groups (p < 0.05). In contrast, no statistical difference was observed between the samples as regards mineralization nodules. Lower biofilm formation of S.a and P.a. was observed on the Nb samples, with statistical differences from the other samples. Conclusion Our results suggest that the basic elements present in the alloy have osteoinductive characteristics, and Zr has a good influence on bone marrow stromal cell differentiation. We also believe that Nb has the best potential for reducing the formation of microbial biofilms.

scholarly journals Prostaglandin E2 Inhibits p53 in Human Breast Adipose Stromal Cells: A Novel Mechanism for the Regulation of Aromatase in Obesity and Breast Cancer

2015 ◽  
Vol 75 (4) ◽  
pp. 645-655 ◽  
Author(s):  
Xuyi Wang ◽  
Maria M. Docanto ◽  
Hironobu Sasano ◽  
Camden Lo ◽  
Evan R. Simpson ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Prostaglandin E2 ◽  
Stromal Cells ◽  
Human Breast ◽  
Adipose Stromal Cells ◽  
Breast Adipose

New potential agents for cryopreservation of hematopoietic and adipose stromal cells

Cytotherapy ◽  
2014 ◽  
Vol 16 (4) ◽  
pp. S58
Author(s):  
J. Svalgaard ◽  
E. Haastrup ◽  
A. Brink ◽  
C. Clausen ◽  
B. Holst ◽  
...  
Keyword(s):  
Stromal Cells ◽  
Adipose Stromal Cells
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