Implanted Bioelectric Neuro Assay with Sensing Interface Circuit

2020 ◽  
Vol 18 (9) ◽  
pp. 686-693
Author(s):  
Suw Young Ly ◽  
Hyeon Jeong Park ◽  
Celina Jae Won Jang ◽  
Katlynn Ryu ◽  
Woo Seok Kim ◽  
...  

Neuromolecular glucose and dopamine assays were searched using a DNA immobilized onto a carbon nanotube paste electrode (PE). The analytical molecular detection limits of 0.13 ugL–1(6.855 × 10–10 M) Dopamine and 1.9 ugL–1 (1.06 × 10–8 M) glucose were attained using square wave stripping voltammetry. A handmade three-electrode system was implanted in the nerve network of a fish backbone, and two working electrodes were implanted in left and right pinna muscles. These were interfaced with a neuron electrochemical workstation and a nerve machine sensing circuit. This interface could be obtained for the psychological function and other body functions. The interfaced circuit could be controlled with a machine system. The results are useful in machine brain intercontrol systems.

2008 ◽  
Vol 61 (10) ◽  
pp. 826 ◽  
Author(s):  
Suw Young Ly ◽  
Young Sam Jung ◽  
Chang Hyun Lee ◽  
Bang Won Lee

An analytical pesticide assay of O-ethyl-O-4-(nitrophenyl)phenyl phosphonothioate (EPN) was carried out using the following: a carbon nanotube paste electrode, a mercury-immobilized carbon nanotube paste electrode, a glassy carbon electrode, a metal–gold electrode, and a DNA-immobilized carbon nanotube paste electrode (DPE), which is two-fold more sensitive than other sensors. The DPE was optimized using cyclic and square wave stripping voltammetry. Linear working ranges approached 5–55 mg L–1 EPN and the nano-range of 10–210 ng L–1 in a 0.1 mol L–1 NH4H2PO4 electrolyte solution, with a speedy analytical time of 30-s stripping. The detection limit was 2.57 ng L–1 (7.94 × 10–12 mol L–1), and the precision was 0.102% relative standard deviation (n = 15) at the 10.0 mg L–1 EPN spike. This indicates that the method is more sensitive than common voltammetric methods. This method was applied to fruit samples using patch- and needle-type electrodes, specifically on the skin tissues of an orange and an apple. Moreover, the implanted electrode was interfaced with a fish brain cell at the electrochemical workstation. Results showed that the aforementioned method can be used to conduct a pesticide assay in neuro-treated and non-treated cell systems.


2017 ◽  
Vol 240 ◽  
pp. 848-856 ◽  
Author(s):  
Mohamad Idris Saidin ◽  
Illyas Md Isa ◽  
Mustaffa Ahmad ◽  
Norhayati Hashim ◽  
Sulaiman Ab Ghani

2010 ◽  
Vol 83 (11) ◽  
pp. 1364-1366 ◽  
Author(s):  
Camila Bitencourt Mendes ◽  
Felipe Nascimento Andrade ◽  
Mariana Gava Segatelli ◽  
Arnaldo César Pereira ◽  
Douglas Cardoso Dragunski ◽  
...  

2011 ◽  
Vol 76 (12) ◽  
pp. 1699-1715 ◽  
Author(s):  
Sławomira Skrzypek ◽  
Valentin Mirceski ◽  
Sylwia Smarzewska ◽  
Dariusz Guziejewski ◽  
Witold Ciesielski

Although 2-guanidinobenzimidazole (GBI; CAS: 5418-95-1) is a compound of biological interest, generally there is a lack of electrochemical studies and the methods of its determination. The GBI behavior at a mercury electrode was analyzed under conditions of linear sweep voltammetry (LSV), differential pulse voltammetry (DPV), square-wave voltammetry (SWV) and square-wave stripping voltammetry (SWSV). Although GBI is electrochemically inactive at mercury electrode it adsorbs at the mercury surface and catalyzes effectively the hydrogen evolution reaction. Theoretical analysis of two possible pathways, according to which the GBI electrode mechanism can be explained, is performed. Simple analysis of peak current and potential with respect to available time window, i.e. change of frequency can be helpful in discerning the character of the recorded SW current. The established electrode mechanism is assumed to involve a preceding chemical reaction in which the adsorbed catalyst (GBIads) is protonated and the protonated form of the catalyst (GBIH+(ads)) is irreversibly reduced at potential about –1.18 V vs Ag|AgCl (citrate buffer pH 2.5). New methods of voltammetric determination of 2-guanidinobenzimidazole were developed. The detection and quantifications limits were found to be 1 × 10–7, 1 × 10–6 mol l–1 (SWV); 8 × 10–8, 9 × 10–7 mol l–1 (SWSV); 4 × 10–7, 2 × 10–6 mol l–1 (DPV) and 6 × 10–7, 3 × 10–6 mol l–1 (LSV), respectively.


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