A Photochromic Fluorescent Probe for Hg2+ Based on Dithienylethene-Rhodamine B Dyad and Its Application in Live Cells Imaging

2017 ◽  
Vol 9 (3) ◽  
pp. 533-540 ◽  
Author(s):  
Sheng Wang ◽  
Chengpeng Li ◽  
Li Xu ◽  
Tang Xu ◽  
Yingnian Lv ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Rhodamine B ◽  
Live Cells

Synthesis and Characterization of ROSA Dye - A Rhodamine B-type Fluorophore, Suitable for Bioconjugation and Fluorescence Studies in Live Cells

2019 ◽  
Vol 26 (10) ◽  
pp. 758-767
Author(s):  
Vicente Rubio ◽  
Vijaya Iragavarapu ◽  
Maciej J. Stawikowski
Keyword(s):  
Quantum Yield ◽  
Cancer Cells ◽  
Fluorescent Probe ◽  
Extinction Coefficient ◽  
Rhodamine B ◽  
Solid Phase ◽  
Total Yield ◽  
Molar Extinction Coefficient ◽  
Αvβ3 Integrin ◽  
Αvβ3 Integrins

Background: Herein we report the multigram-scale synthesis, characterization and application of a rhodamine B-based fluorophore (ROSA) suitable for fluorescent studies in biological applications. This fluorophore is devoid of rhodamine spirolactone formation and furthermore characterized by a high molar extinction coefficient (ϵ=87250 ± 1630 M-1cm-1) and quantum yield (φ) of 0.589 ± 0.070 in water. Reported here is also the application of ROSA towards synthesis of a ROSA-PEG-GRGDS-NH2 fluorescent probe suitable for live cell imaging of αvβ3 integrins for in vitro assays. Objective: The main objective of this study is to efficiently prepare rhodamine B derivative, devoid of spirolactone formation that would be suitable for bioconjugation and subsequent bioimaging. Methods: Rhodamine B was transformed into rhodamine B succinimide ester (RhoB-OSu) using N-hydroxysuccinimide. RhoB-OSu was further coupled to sarcosine to obtain rhodamine Bsarcosine dye (ROSA) in good yield. The ROSA dye was then coupled to a αvβ3 integrin binding sequence using standard solid-phase conditions. Resulting ROSA-PEG-GRGDS-NH2 probe was used to image integrins on cancer cells. Results: The rhodamine B-sarcosine dye (ROSA) was obtained in multigram scale in good total yield of 47%. Unlike rhodamine B, the ROSA dye does not undergo pH-dependent spirolactone/spirolactam formation as compared with rhodamine B-glycine. It is also characterized by excellent quantum yield (φ) of 0.589 ± 0.070 in water and high molar extinction coefficient of 87250 ± 1630 M-1cm-1. ROSA coupling to the RGD-like peptide was proved to be efficient and straightforward. Imaging using standard filters on multimode plate reader and confocal microscope was performed. The αvβ3 integrins present on the surface of live WM-266-4 (melanoma) and MCF- 7 (breast cancer) cells were successfully imaged. Conclusion: We successfully derivatized rhodamine B to create an inexpensive, stable and convenient to use fluorescent probe. The obtained derivative has excellent photochemical properties and it is suitable for bioconjugation and many imaging applications.

Turn-on fluorescent probe for dopamine detection in solutions and live cells based on in situ formation of aminosilane-functionalized carbon dots

2021 ◽  
Vol 1157 ◽  
pp. 338394
Author(s):  
Xiao-Yue Tang ◽  
Yi-Ming Liu ◽  
Xiao-Lin Bai ◽  
Hao Yuan ◽  
Yi-Kao Hu ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Carbon Dots ◽  
Live Cells ◽  
Turn On ◽  
Dopamine Detection ◽  
In Situ Formation

A water-soluble boronate masked benzoindocyanin fluorescent probe for the detection of endogenous mitochondrial peroxynitrite in live cells and zebrafish as inflammation models

2021 ◽  
pp. 109371
Author(s):  
Prasad M. Sonawane ◽  
Tesla Yudhistira ◽  
Mahesh B. Halle ◽  
Arkaprava Roychaudhury ◽  
Yunsu Kim ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Water Soluble ◽  
Live Cells

scholarly journals A Fluorescent Probe with Improved Water Solubility Permits the Analysis of Protein S-Depalmitoylation Activity in Live Cells

Biochemistry ◽  
2017 ◽  
Vol 57 (2) ◽  
pp. 221-225 ◽  
Author(s):  
Tian Qiu ◽  
Rahul S. Kathayat ◽  
Yang Cao ◽  
Michael W. Beck ◽  
Bryan C. Dickinson
Keyword(s):  
Fluorescent Probe ◽  
Water Solubility ◽  
Protein S ◽  
Live Cells

A BODIPY-based two-photon fluorescent probe validates tyrosinase activity in live cells

2017 ◽  
Vol 53 (81) ◽  
pp. 11213-11216 ◽  
Author(s):  
Kondapa Naidu Bobba ◽  
Miae Won ◽  
Inseob Shim ◽  
Nithya Velusamy ◽  
Zhigang Yang ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Melanoma Cells ◽  
Tyrosinase Activity ◽  
Live Cells ◽  
Two Photon

A two-photon fluorescent probe (Tyro-1) can validate intracellular tyrosinase activity, and H2O2induced upregulated tyrosinase in melanoma cells.

A novel cell membrane-targeting fluorescent probe for imaging endogenous/exogenous formaldehyde in live cells and zebrafish

The Analyst ◽  
2021 ◽  
Author(s):  
Wenlong Sheng ◽  
Xue Zhang ◽  
Miaohui Yu ◽  
Meng Jin ◽  
Ning Li ◽  
...  
Keyword(s):  
Economic Development ◽  
Cell Membrane ◽  
Human Health ◽  
Fluorescent Probe ◽  
Live Cells ◽  
Membrane Targeting ◽  
Health It ◽  
Global Pollutant

Formaldehyde (FA) is not only an economic chemical but also a global pollutant. Although it brings economic development, it poses a threat to human health. It is also a kind...

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