scholarly journals A Fluorescent Probe with Improved Water Solubility Permits the Analysis of Protein S-Depalmitoylation Activity in Live Cells

Biochemistry ◽  
2017 ◽  
Vol 57 (2) ◽  
pp. 221-225 ◽  
Author(s):  
Tian Qiu ◽  
Rahul S. Kathayat ◽  
Yang Cao ◽  
Michael W. Beck ◽  
Bryan C. Dickinson
Keyword(s):  
Fluorescent Probe ◽  
Water Solubility ◽  
Protein S ◽  
Live Cells

Turn-on fluorescent probe for dopamine detection in solutions and live cells based on in situ formation of aminosilane-functionalized carbon dots

2021 ◽  
Vol 1157 ◽  
pp. 338394
Author(s):  
Xiao-Yue Tang ◽  
Yi-Ming Liu ◽  
Xiao-Lin Bai ◽  
Hao Yuan ◽  
Yi-Kao Hu ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Carbon Dots ◽  
Live Cells ◽  
Turn On ◽  
Dopamine Detection ◽  
In Situ Formation

A water-soluble boronate masked benzoindocyanin fluorescent probe for the detection of endogenous mitochondrial peroxynitrite in live cells and zebrafish as inflammation models

2021 ◽  
pp. 109371
Author(s):  
Prasad M. Sonawane ◽  
Tesla Yudhistira ◽  
Mahesh B. Halle ◽  
Arkaprava Roychaudhury ◽  
Yunsu Kim ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Water Soluble ◽  
Live Cells

scholarly journals Hydrogen Sulfide Increases Nitric Oxide Production and Subsequent S-Nitrosylation in Endothelial Cells

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Ping-Ho Chen ◽  
Yaw-Syan Fu ◽  
Yun-Ming Wang ◽  
Kun-Han Yang ◽  
Danny Ling Wang ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Endothelial Cells ◽  
Hydrogen Sulfide ◽  
Protein Kinase ◽  
Fluorescent Probe ◽  
Acid Methyl Ester ◽  
High Specificity ◽  
Protein S ◽  
No Production ◽  
No Donor

Hydrogen sulfide (H2S) and nitric oxide (NO), two endogenous gaseous molecules in endothelial cells, got increased attention with respect to their protective roles in the cardiovascular system. However, the details of the signaling pathways between H2S and NO in endothelia cells remain unclear. In this study, a treatment with NaHS profoundly increased the expression and the activity of endothelial nitric oxide synthase. Elevated gaseous NO levels were observed by a novel and specific fluorescent probe, 5-amino-2-(6-hydroxy-3-oxo-3H-xanthen-9-yl)benzoic acid methyl ester (FA-OMe), and quantified by flow cytometry. Further study indicated an increase of upstream regulator for eNOS activation, AMP-activated protein kinase (AMPK), and protein kinase B (Akt). By using a biotin switch, the level of NO-mediated protein S-nitrosylation was also enhanced. However, with the addition of the NO donor, NOC-18, the expressions of cystathionine-γ-lyase, cystathionine-β-synthase, and 3-mercaptopyruvate sulfurtransferase were not changed. The level of H2S was also monitored by a new designed fluorescent probe, 4-nitro-7-thiocyanatobenz-2-oxa-1,3-diazole (NBD-SCN) with high specificity. Therefore, NO did not reciprocally increase the expression of H2S-generating enzymes and the H2S level. The present study provides an integrated insight of cellular responses to H2S and NO from protein expression to gaseous molecule generation, which indicates the upstream role of H2S in modulating NO production and protein S-nitrosylation.

A BODIPY-based two-photon fluorescent probe validates tyrosinase activity in live cells

2017 ◽  
Vol 53 (81) ◽  
pp. 11213-11216 ◽  
Author(s):  
Kondapa Naidu Bobba ◽  
Miae Won ◽  
Inseob Shim ◽  
Nithya Velusamy ◽  
Zhigang Yang ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Melanoma Cells ◽  
Tyrosinase Activity ◽  
Live Cells ◽  
Two Photon

A two-photon fluorescent probe (Tyro-1) can validate intracellular tyrosinase activity, and H2O2induced upregulated tyrosinase in melanoma cells.

A novel cell membrane-targeting fluorescent probe for imaging endogenous/exogenous formaldehyde in live cells and zebrafish

The Analyst ◽  
2021 ◽  
Author(s):  
Wenlong Sheng ◽  
Xue Zhang ◽  
Miaohui Yu ◽  
Meng Jin ◽  
Ning Li ◽  
...  
Keyword(s):  
Economic Development ◽  
Cell Membrane ◽  
Human Health ◽  
Fluorescent Probe ◽  
Live Cells ◽  
Membrane Targeting ◽  
Health It ◽  
Global Pollutant

Formaldehyde (FA) is not only an economic chemical but also a global pollutant. Although it brings economic development, it poses a threat to human health. It is also a kind...

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