Interaction of a pH-Responsive Designed Nanostructured Peptide with a Model Lipid Membrane

2016 ◽  
Vol 16 (8) ◽  
pp. 8528-8532
Author(s):  
Givaldo C Amaral ◽  
Analu R Costa ◽  
Paulo R. S Sanches ◽  
Eduardo M Cilli ◽  
Luiz C Salay
Keyword(s):  
Lipid Membrane ◽  
Ph Responsive

scholarly journals Dynamic studies of the interaction of a pH responsive, amphiphilic polymer with a DOPC lipid membrane

Soft Matter ◽  
2017 ◽  
Vol 13 (20) ◽  
pp. 3690-3700 ◽  
Author(s):  
Sivaramakrishnan Ramadurai ◽  
Marco Werner ◽  
Nigel K. H. Slater ◽  
Aaron Martin ◽  
Vladimir A. Baulin ◽  
...  
Keyword(s):  
Lipid Membrane ◽  
Amphiphilic Polymer ◽  
Ph Responsive ◽  
Dynamic Studies

Structural Effects and Lipid Membrane Interactions of the pH-Responsive GALA Peptide with Fatty Acid Acylation

Biochemistry ◽  
2012 ◽  
Vol 51 (23) ◽  
pp. 4658-4668 ◽  
Author(s):  
Brian F. Lin ◽  
Dimitris Missirlis ◽  
Daniel V. Krogstad ◽  
Matthew Tirrell
Keyword(s):  
Fatty Acid ◽  
Lipid Membrane ◽  
Ph Responsive ◽  
Structural Effects ◽  
Membrane Interactions

Molecular Interactions of an Ornithine-Rich pH-Responsive Self- Assembling Peptide with a Model Lipid Membrane: Conformational Aspects

2016 ◽  
Vol 23 (9) ◽  
pp. 790-794 ◽  
Author(s):  
Glauber G. Nascimento ◽  
Luiz C. Salay ◽  
Paulo R.S. Sanches ◽  
Eduardo M. Cilli
Keyword(s):  
Molecular Interactions ◽  
Lipid Membrane ◽  
Ph Responsive ◽  
Self Assembling

Chapter 2b: The molecular antigenic structure of the TBEV

2020 ◽  
Keyword(s):  
Conformational Changes ◽  
Neutralizing Antibodies ◽  
Lipid Membrane ◽  
Cell Entry ◽  
Antigenic Structure ◽  
E Proteins ◽  
Amino Acid Sequence Level ◽  
Endosomal Membrane ◽  
E Protein ◽  
Golgi Network

TBEV-particles are assembled in an immature, noninfectious form in the endoplasmic reticulum by the envelopment of the viral core (containing the viral RNA) by a lipid membrane associated with two viral proteins, prM and E. Immature particles are transported through the cellular exocytic pathway and conformational changes induced by acidic pH in the trans-Golgi network allow the proteolytic cleavage of prM by furin, a cellular protease, resulting in the release of mature and infectious TBE-virions. The E protein controls cell entry by mediating attachment to as yet ill-defined receptors as well as by low-pH-triggered fusion of the viral and endosomal membrane after uptake by receptor-mediated endocytosis. Because of its key functions in cell entry, the E protein is the primary target of virus neutralizing antibodies, which inhibit these functions by different mechanisms. Although all flavivirus E proteins have a similar overall structure, divergence at the amino acid sequence level is up to 60 percent (e.g. between TBE and dengue viruses), and therefore cross-neutralization as well as (some degree of) cross-protection are limited to relatively closely related flaviviruses, such as those constituting the tick-borne encephalitis serocomplex.

Electrophysiological Characterization of Transport Across Outer Membrane Channels from Gram-Negative Bacteria in Their Native Environment

2019 ◽  
Author(s):  
Jiajun Wang ◽  
Rémi Terrasse ◽  
Jayesh Arun Bafna ◽  
Lorraine Benier ◽  
Mathias Winterhalter
Keyword(s):  
Outer Membrane ◽  
Lipid Membrane ◽  
Membrane Vesicles ◽  
Outer Membrane Vesicles ◽  
Multi Drug Resistance ◽  
Gram Negative Bacteria ◽  
Membrane Channels ◽  
Gram Negative ◽  
Electrophysiological Characterization

Multi-drug resistance in Gram-negative bacteria is often associated with low permeability of the outer membrane. To investigate the role of membrane channels in the uptake of antibiotics, we extract, purify and reconstitute them into artificial planar membranes. To avoid this time-consuming procedure, here we show a robust approach using fusion of native outer membrane vesicles (OMV) into planar lipid bilayer which moreover allows also to some extend the characterization of membrane protein channels in their native environment. Two major membrane channels from <i>Escherichia coli</i>, OmpF and OmpC, were overexpressed from the host and the corresponding OMVs were collected. Each OMV fusion revealed surprisingly single or only few channel activities. The asymmetry of the OMV´s translates after fusion into the lipid membrane with the LPS dominantly present at the side of OMV addition. Compared to conventional reconstitution methods, the channels fused from OMVs containing LPS have similar conductance but a much broader distribution. The addition of Enrofloxacin on the LPS side yields somewhat higher association (<i>k<sub>on</sub></i>) and lower dissociation (<i>k<sub>off</sub></i>) rates compared to LPS-free reconstitution. We conclude that using outer membrane vesicles is a fast and easy approach for functional and structural studies of membrane channels in the native membrane.

Capacitance extraction method for a free-standing bilayer lipid membrane formed over an aperture in a nanofabricated silicon chip

2020 ◽  
Vol 59 (SI) ◽  
pp. SIIK02
Author(s):  
Yasutaka Tomioka ◽  
Shogo Takashima ◽  
Masataka Moriya ◽  
Hiroshi Shimada ◽  
Fumihiko Hirose ◽  
...  
Keyword(s):  
Extraction Method ◽  
Lipid Membrane ◽  
Bilayer Lipid Membrane ◽  
Free Standing ◽  
Capacitance Extraction ◽  
Bilayer Lipid
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