Structural Effects and Lipid Membrane Interactions of the pH-Responsive GALA Peptide with Fatty Acid Acylation

Biochemistry ◽  
2012 ◽  
Vol 51 (23) ◽  
pp. 4658-4668 ◽  
Author(s):  
Brian F. Lin ◽  
Dimitris Missirlis ◽  
Daniel V. Krogstad ◽  
Matthew Tirrell
Keyword(s):  
Fatty Acid ◽  
Lipid Membrane ◽  
Ph Responsive ◽  
Structural Effects ◽  
Membrane Interactions

scholarly journals Menaquinone-mediated regulation of membrane fluidity is relevant for fitness of Listeria monocytogenes

2021 ◽  
Author(s):  
Alexander Flegler ◽  
Vanessa Kombeitz ◽  
André Lipski
Keyword(s):  
Fatty Acid ◽  
Listeria Monocytogenes ◽  
Membrane Fluidity ◽  
Low Temperatures ◽  
Lipid Membrane ◽  
Feedback Inhibition ◽  
Aromatic Amino Acids ◽  
Adaptation Effect ◽  
Membrane Composition ◽  
Lower Growth

AbstractListeria monocytogenes is a food-borne pathogen with the ability to grow at low temperatures down to − 0.4 °C. Maintaining cytoplasmic membrane fluidity by changing the lipid membrane composition is important during growth at low temperatures. In Listeria monocytogenes, the dominant adaptation effect is the fluidization of the membrane by shortening of fatty acid chain length. In some strains, however, an additional response is the increase in menaquinone content during growth at low temperatures. The increase of this neutral lipid leads to fluidization of the membrane and thus represents a mechanism that is complementary to the fatty acid-mediated modification of membrane fluidity. This study demonstrated that the reduction of menaquinone content for Listeria monocytogenes strains resulted in significantly lower resistance to temperature stress and lower growth rates compared to unaffected control cultures after growth at 6 °C. Menaquinone content was reduced by supplementation with aromatic amino acids, which led to a feedback inhibition of the menaquinone synthesis. Menaquinone-reduced Listeria monocytogenes strains showed reduced bacterial cell fitness. This confirmed the adaptive function of menaquinones for growth at low temperatures of this pathogen.

Novel fatty acid-based pH-responsive nanostructured lipid carriers for enhancing antibacterial delivery

2019 ◽  
Vol 53 ◽  
pp. 101125 ◽  
Author(s):  
Nawras Osman ◽  
Calvin A. Omolo ◽  
Ramesh Gannimani ◽  
Ayman Y. Waddad ◽  
Sanjeev Rambharose ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Nanostructured Lipid Carriers ◽  
Ph Responsive

Abstract 631: Lipid Composition of Gold Nanoparticle-Templated High-Density Lipoprotein Analogs Dictates Cholesterol Efflux Function

2014 ◽  
Vol 34 (suppl_1) ◽  
Author(s):  
R Kannan Mutharasan ◽  
Amritha T Singh ◽  
Kaylin M McMahon ◽  
C Shad Thaxton
Keyword(s):  
Fatty Acid ◽  
Lipid Content ◽  
Lipid Composition ◽  
Reverse Cholesterol Transport ◽  
Cholesterol Efflux ◽  
Lipid Membrane ◽  
Density Lipoprotein ◽  
High Density ◽  
High Density Lipoproteins ◽  
Cholesterol Transport

Background: Reverse cholesterol transport, the process by which cholesterol is effluxed from cells to high-density lipoproteins (HDL) and is delivered to the liver for clearance, is a promising pathway to augment for treatment of atherosclerosis. Though structure-function relationships for nascent, discoidal HDL and cholesterol efflux have been well studied, how the lipid composition of spherical HDL species - which varies in pathophysiological conditions - impacts their ability to mediate cholesterol efflux has not been investigated. Methods and Results: Spherical gold nanoparticles (5 nm) were used to synthesize spherical HDL analogs (HDL-NP) by adding ApoAI protein, and various lipids. With this strategy a panel of HDL-NP varying in lipid content was generated. HDL-NP designs tested include: dipalmitylphosphatidylcholine (DPPC, saturated fatty acid), dioleoylphosphatidylcholine (DOPC, unsaturated fatty acid), sphingomyelin, lysophosphatidylcholine (LPC), and mixtures thereof. All of these species are found in natural HDL. After characterizing protein and lipid stoichiometry of the purified HDL-NP, these HDL-NP designs were tested in the cellular reverse cholesterol transport assay using J774 mouse macrophages. These studies demonstrate that all HDL-NP designs mediate more efflux than equimolar amounts of ApoAI protein control, and further demonstrate that HDL-NP designs incorporating unsaturated phospholipid (DOPC), sphingomyelin, and LPC - each of which can increase disorder in the lipid membrane and thus give rise to opportunity for cholesterol to intercalate and bind - enhance cholesterol efflux compared to saturated phospholipid (DPPC) design. Conclusion: In summary, these results demonstrate that lipid content of HDL-NP - analogs of spherical HDL - dictates cholesterol efflux function, a finding which sheds light on the functional importance of lipid content variation seen in mature, spherical HDL species.

DAP derived fatty acid amide organogelators as novel carrier for drug incorporation and pH-responsive release

2021 ◽  
Vol 45 (1) ◽  
pp. 415-422
Author(s):  
Eqvinshi Yadav ◽  
Anil Kumar Khatana ◽  
Sharol Sebastian ◽  
Manoj K. Gupta
Keyword(s):  
Fatty Acid ◽  
Molecular Mass ◽  
Acid Amide ◽  
Ph Responsive ◽  
Alkyl Chains ◽  
Drug Incorporation ◽  
Structure And Activity ◽  
Fatty Acid Amide

Low-molecular mass fatty acid amide gelators were synthesized using 2,6-diaminopyridine as a linker and alkyl chains of varying lengths. The prepared organogel-elusions are able to trap and release ibuprofen molecule without changing its structure and activity.

Characterization of pH-Responsive Self-Assembly Behaviors of Fatty Acid-Functionalized Prodrug

2020 ◽  
Vol 164 ◽  
pp. 107794
Author(s):  
Jin Han ◽  
Keita Hayashi ◽  
Yukihiro Okamoto ◽  
Keishi Suga ◽  
Hiroshi Umakoshi
Keyword(s):  
Fatty Acid ◽  
Self Assembly ◽  
Ph Responsive

pH-Responsive Jello: Gelatin Gels Containing Fatty Acid Vesicles†

Langmuir ◽  
2009 ◽  
Vol 25 (15) ◽  
pp. 8519-8525 ◽  
Author(s):  
Matthew B. Dowling ◽  
Jae-Ho Lee ◽  
Srinivasa R. Raghavan
Keyword(s):  
Fatty Acid ◽  
Ph Responsive ◽  
Gelatin Gels
Sign in / Sign up

Export Citation Format

Share Document