An Integrated Microfluidic Device for Rapid Cell Lysis and DNA Purification of Epithelial Cell Samples

2011 ◽  
Vol 11 (5) ◽  
pp. 4250-4253 ◽  
Author(s):  
Seung-Mo Ha ◽  
Woong Cho ◽  
Yoomin Ahn ◽  
Seung Yong Hwang
Keyword(s):  
Epithelial Cell ◽  
Microfluidic Device ◽  
Cell Lysis ◽  
Dna Purification

Continuous flow microfluidic device for cell separation, cell lysis and DNA purification

2007 ◽  
Vol 584 (2) ◽  
pp. 237-243 ◽  
Author(s):  
Xing Chen ◽  
Dafu Cui ◽  
Changchun Liu ◽  
Hui Li ◽  
Jian Chen
Keyword(s):  
Microfluidic Device ◽  
Continuous Flow ◽  
Cell Separation ◽  
Cell Lysis ◽  
Dna Purification ◽  
Separation Cell

scholarly journals Design and Simulation of an Integrated Centrifugal Microfluidic Device for CTCs Separation and Cell Lysis

Micromachines ◽  
2020 ◽  
Vol 11 (7) ◽  
pp. 699
Author(s):  
Rohollah Nasiri ◽  
Amir Shamloo ◽  
Javad Akbari ◽  
Peyton Tebon ◽  
Mehmet R. Dokmeci ◽  
...  
Keyword(s):  
Numerical Simulation ◽  
Angular Velocity ◽  
Microfluidic Device ◽  
Cell Separation ◽  
High Efficiency ◽  
Separation Efficiency ◽  
White Blood Cells ◽  
Cell Lysis ◽  
Target Cells ◽  
Separation Unit

Separation of circulating tumor cells (CTCs) from blood samples and subsequent DNA extraction from these cells play a crucial role in cancer research and drug discovery. Microfluidics is a versatile technology that has been applied to create niche solutions to biomedical applications, such as cell separation and mixing, droplet generation, bioprinting, and organs on a chip. Centrifugal microfluidic biochips created on compact disks show great potential in processing biological samples for point of care diagnostics. This study investigates the design and numerical simulation of an integrated microfluidic device, including a cell separation unit for isolating CTCs from a blood sample and a micromixer unit for cell lysis on a rotating disk platform. For this purpose, an inertial microfluidic device was designed for the separation of target cells by using contraction–expansion microchannel arrays. Additionally, a micromixer was incorporated to mix separated target cells with the cell lysis chemical reagent to dissolve their membranes to facilitate further assays. Our numerical simulation approach was validated for both cell separation and micromixer units and corroborates existing experimental results. In the first compartment of the proposed device (cell separation unit), several simulations were performed at different angular velocities from 500 rpm to 3000 rpm to find the optimum angular velocity for maximum separation efficiency. By using the proposed inertial separation approach, CTCs, were successfully separated from white blood cells (WBCs) with high efficiency (~90%) at an angular velocity of 2000 rpm. Furthermore, a serpentine channel with rectangular obstacles was designed to achieve a highly efficient micromixer unit with high mixing quality (~98%) for isolated CTCs lysis at 2000 rpm.

Wireless induction heating in a microfluidic device for cell lysis

Lab on a Chip ◽  
2010 ◽  
Vol 10 (7) ◽  
pp. 909 ◽  
Author(s):  
Seung-ki Baek ◽  
Junghong Min ◽  
Jung-Hwan Park
Keyword(s):  
Microfluidic Device ◽  
Induction Heating ◽  
Cell Lysis

Integrated Microfluidic Device for Cell Lysis in a Continuous Flow Mode

2015 ◽  
Vol 4 (2) ◽  
pp. 150-153 ◽  
Author(s):  
Nhut Tran-Minh ◽  
Birgitte Kasin Hønsvall ◽  
Frank Karlsen
Keyword(s):  
Microfluidic Device ◽  
Continuous Flow ◽  
Cell Lysis ◽  
Flow Mode

Single cell lysis and DNA extending using electroporation microfluidic device

2012 ◽  
Vol 6 (1) ◽  
pp. 84-90 ◽  
Author(s):  
Min-Sheng Hung ◽  
Ya-Tun Chang
Keyword(s):  
Single Cell ◽  
Microfluidic Device ◽  
Cell Lysis

Low-voltage electrical cell lysis using a microfluidic device

2019 ◽  
Vol 21 (1) ◽  
Author(s):  
Xiao-yu Wei ◽  
Jin-hua Li ◽  
Lei Wang ◽  
Fang Yang
Keyword(s):  
Microfluidic Device ◽  
Low Voltage ◽  
Cell Lysis

Bead beating-based continuous flow cell lysis in a microfluidic device

RSC Advances ◽  
2015 ◽  
Vol 5 (29) ◽  
pp. 22350-22355 ◽  
Author(s):  
A. Berasaluce ◽  
L. Matthys ◽  
J. Mujika ◽  
M. Antoñana-Díez ◽  
A. Valero ◽  
...  
Keyword(s):  
Microfluidic Device ◽  
Continuous Flow ◽  
Previous Treatment ◽  
Cell Lysis ◽  
Flow Cell ◽  
Bead Beating

This paper describes a bead beating-based miniaturized cell lysis device that works in continuous flow allowing the analysis of large volumes of samples without previous treatment.

Simultaneous cell lysis and bead trapping in a continuous flow microfluidic device

2006 ◽  
Vol 113 (2) ◽  
pp. 944-955 ◽  
Author(s):  
Qasem Ramadan ◽  
Victor Samper ◽  
Daniel Poenar ◽  
Zhu Liang ◽  
Chen Yu ◽  
...  
Keyword(s):  
Microfluidic Device ◽  
Continuous Flow ◽  
Cell Lysis
Sign in / Sign up

Export Citation Format

Share Document