Continuous flow microfluidic device for cell separation, cell lysis and DNA purification

2007 ◽  
Vol 584 (2) ◽  
pp. 237-243 ◽  
Author(s):  
Xing Chen ◽  
Dafu Cui ◽  
Changchun Liu ◽  
Hui Li ◽  
Jian Chen
Keyword(s):  
Microfluidic Device ◽  
Continuous Flow ◽  
Cell Separation ◽  
Cell Lysis ◽  
Dna Purification ◽  
Separation Cell

scholarly journals Design and Simulation of an Integrated Centrifugal Microfluidic Device for CTCs Separation and Cell Lysis

Micromachines ◽  
2020 ◽  
Vol 11 (7) ◽  
pp. 699
Author(s):  
Rohollah Nasiri ◽  
Amir Shamloo ◽  
Javad Akbari ◽  
Peyton Tebon ◽  
Mehmet R. Dokmeci ◽  
...  
Keyword(s):  
Numerical Simulation ◽  
Angular Velocity ◽  
Microfluidic Device ◽  
Cell Separation ◽  
High Efficiency ◽  
Separation Efficiency ◽  
White Blood Cells ◽  
Cell Lysis ◽  
Target Cells ◽  
Separation Unit

Separation of circulating tumor cells (CTCs) from blood samples and subsequent DNA extraction from these cells play a crucial role in cancer research and drug discovery. Microfluidics is a versatile technology that has been applied to create niche solutions to biomedical applications, such as cell separation and mixing, droplet generation, bioprinting, and organs on a chip. Centrifugal microfluidic biochips created on compact disks show great potential in processing biological samples for point of care diagnostics. This study investigates the design and numerical simulation of an integrated microfluidic device, including a cell separation unit for isolating CTCs from a blood sample and a micromixer unit for cell lysis on a rotating disk platform. For this purpose, an inertial microfluidic device was designed for the separation of target cells by using contraction–expansion microchannel arrays. Additionally, a micromixer was incorporated to mix separated target cells with the cell lysis chemical reagent to dissolve their membranes to facilitate further assays. Our numerical simulation approach was validated for both cell separation and micromixer units and corroborates existing experimental results. In the first compartment of the proposed device (cell separation unit), several simulations were performed at different angular velocities from 500 rpm to 3000 rpm to find the optimum angular velocity for maximum separation efficiency. By using the proposed inertial separation approach, CTCs, were successfully separated from white blood cells (WBCs) with high efficiency (~90%) at an angular velocity of 2000 rpm. Furthermore, a serpentine channel with rectangular obstacles was designed to achieve a highly efficient micromixer unit with high mixing quality (~98%) for isolated CTCs lysis at 2000 rpm.

Integrated Microfluidic Device for Cell Lysis in a Continuous Flow Mode

2015 ◽  
Vol 4 (2) ◽  
pp. 150-153 ◽  
Author(s):  
Nhut Tran-Minh ◽  
Birgitte Kasin Hønsvall ◽  
Frank Karlsen
Keyword(s):  
Microfluidic Device ◽  
Continuous Flow ◽  
Cell Lysis ◽  
Flow Mode

Bead beating-based continuous flow cell lysis in a microfluidic device

RSC Advances ◽  
2015 ◽  
Vol 5 (29) ◽  
pp. 22350-22355 ◽  
Author(s):  
A. Berasaluce ◽  
L. Matthys ◽  
J. Mujika ◽  
M. Antoñana-Díez ◽  
A. Valero ◽  
...  
Keyword(s):  
Microfluidic Device ◽  
Continuous Flow ◽  
Previous Treatment ◽  
Cell Lysis ◽  
Flow Cell ◽  
Bead Beating

This paper describes a bead beating-based miniaturized cell lysis device that works in continuous flow allowing the analysis of large volumes of samples without previous treatment.

Simultaneous cell lysis and bead trapping in a continuous flow microfluidic device

2006 ◽  
Vol 113 (2) ◽  
pp. 944-955 ◽  
Author(s):  
Qasem Ramadan ◽  
Victor Samper ◽  
Daniel Poenar ◽  
Zhu Liang ◽  
Chen Yu ◽  
...  
Keyword(s):  
Microfluidic Device ◽  
Continuous Flow ◽  
Cell Lysis

An Integrated Microfluidic Device for Rapid Cell Lysis and DNA Purification of Epithelial Cell Samples

2011 ◽  
Vol 11 (5) ◽  
pp. 4250-4253 ◽  
Author(s):  
Seung-Mo Ha ◽  
Woong Cho ◽  
Yoomin Ahn ◽  
Seung Yong Hwang
Keyword(s):  
Epithelial Cell ◽  
Microfluidic Device ◽  
Cell Lysis ◽  
Dna Purification

Functional Magnetite Nanoparticle: A Review on the Particles Lysis and Nucleic Acid Separation

2021 ◽  
Vol 33 ◽  
pp. 13-27
Author(s):  
Puspita Nurlilasari ◽  
Camellia Panatarani ◽  
Mia Miranti ◽  
Savira Ekawardhani ◽  
Ferry Faizal ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Magnetite Nanoparticles ◽  
Magnetic Beads ◽  
Functional Materials ◽  
Cell Lysis ◽  
Acid Extraction ◽  
Nucleic Acid Extraction ◽  
Coating Materials ◽  
Magnetite Nanoparticle ◽  
Separation Cell

The functional magnetite nanoparticles are one of the most important functional materials for nucleic acid separation. Cell lysis and magnetic separation are two essential steps involve in optimizing nucleic acid extraction using the magnetic beads method. Many coating materials, coupling agents, chemical cell lysis, and several methods have been proposed to produce the specific desired properties for nucleic acid extraction. The important properties, such as biocompatibility, stability, linking ability, hydrophobicity, and biodegradable, were considered. The appropriate coating material of magnetite core and coupling agent are necessary to give biomolecules a possibility to link with each other through chemical conjugation. In this review, progress in functional magnetite nanoparticles to optimize the high binding performance in nucleic acid extraction is discussed.

Systematic Use Of Heparin In The Continuous Flow Centrifuge (CFC) For Blood Cell Separation

1981 ◽  
Author(s):  
M R Morales ◽  
J Pizzuto ◽  
Ma Reyna ◽  
G Castro
Keyword(s):  
Blood Cell ◽  
Continuous Flow ◽  
Cell Separation ◽  
Reliable Method ◽  
Dosage Level ◽  
Thrombin Time ◽  
Coagulation Disorders ◽  
Time Dilution ◽  
Continuous Flow Centrifuge ◽  
Simultaneous Assessment

To date the use of heparin in the CFC has not been adequately controlled, thus exposing donors and patients to coagulation disorders. For this reason, we decided to evaluate the use of heparin by continuous infusion in dosages that would be modified by a simultaneous assessment of its anticoagulant effect, as shown by the thrombin time dilution test (TTDT).The study was performed during 46 leukopher- esis and 27 plasmapheresis. It was ascertained that heparin is an efficient anticoagulant in the CFC, using the TTDT as an effective and reliable method for its control. The initial dose in leukopheresis is one unit per milliliter of blood during the first hour, then half the dose during the next hour, and then a quarter of the dose until the procedure is completed. A TTDT performed every hour will indicate whether the amount of heparin used should be modified. For plasmapheresis, it is neccesary to establish a specific dose in each instance, using the TTDT as described. In most of the subjects, the anticoagulant level was exactly right. There was no case of bleeding or extracorporeal coagulation of the blood.On the basis of these findings, we recommend the use of heparin in the CFC, applying the results of the TTDT as a guide for its dosage level.

Continuous-flow, well-mixed, microfluidic crystallization device for screening of polymorphs, morphology, and crystallization kinetics at controlled supersaturation

Lab on a Chip ◽  
2019 ◽  
Vol 19 (14) ◽  
pp. 2373-2382 ◽  
Author(s):  
Paria Coliaie ◽  
Manish S. Kelkar ◽  
Nandkishor K. Nere ◽  
Meenesh R. Singh
Keyword(s):  
Crystallization Kinetics ◽  
Microfluidic Device ◽  
Continuous Flow ◽  
Crystalline Materials ◽  
Screening Techniques

While the conventional screening techniques suffer from depletion of supersaturation, the continuous-flow microfluidic device screens crystalline materials at controlled supersaturation.

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