Bead beating-based continuous flow cell lysis in a microfluidic device

RSC Advances ◽  
2015 ◽  
Vol 5 (29) ◽  
pp. 22350-22355 ◽  
Author(s):  
A. Berasaluce ◽  
L. Matthys ◽  
J. Mujika ◽  
M. Antoñana-Díez ◽  
A. Valero ◽  
...  
Keyword(s):  
Microfluidic Device ◽  
Continuous Flow ◽  
Previous Treatment ◽  
Cell Lysis ◽  
Flow Cell ◽  
Bead Beating

This paper describes a bead beating-based miniaturized cell lysis device that works in continuous flow allowing the analysis of large volumes of samples without previous treatment.

Integrated Microfluidic Device for Cell Lysis in a Continuous Flow Mode

2015 ◽  
Vol 4 (2) ◽  
pp. 150-153 ◽  
Author(s):  
Nhut Tran-Minh ◽  
Birgitte Kasin Hønsvall ◽  
Frank Karlsen
Keyword(s):  
Microfluidic Device ◽  
Continuous Flow ◽  
Cell Lysis ◽  
Flow Mode

Simultaneous cell lysis and bead trapping in a continuous flow microfluidic device

2006 ◽  
Vol 113 (2) ◽  
pp. 944-955 ◽  
Author(s):  
Qasem Ramadan ◽  
Victor Samper ◽  
Daniel Poenar ◽  
Zhu Liang ◽  
Chen Yu ◽  
...  
Keyword(s):  
Microfluidic Device ◽  
Continuous Flow ◽  
Cell Lysis

scholarly journals Fabrication of rigid microstructures with thiol-ene-based soft lithography for continuous-flow cell lysis

2014 ◽  
Vol 8 (5) ◽  
pp. 056503 ◽  
Author(s):  
Jeffrey M. Burke ◽  
Kunal R. Pandit ◽  
John P. Goertz ◽  
Ian M. White
Keyword(s):  
Continuous Flow ◽  
Soft Lithography ◽  
Cell Lysis ◽  
Flow Cell

Continuous flow microfluidic device for cell separation, cell lysis and DNA purification

2007 ◽  
Vol 584 (2) ◽  
pp. 237-243 ◽  
Author(s):  
Xing Chen ◽  
Dafu Cui ◽  
Changchun Liu ◽  
Hui Li ◽  
Jian Chen
Keyword(s):  
Microfluidic Device ◽  
Continuous Flow ◽  
Cell Separation ◽  
Cell Lysis ◽  
Dna Purification ◽  
Separation Cell

Monitoring of Microwave-Assisted Acid-Catalyzed Transesterification for Biodiesel Production Using FT-NIR Spectroscopy with Continuous-Flow Cell

2015 ◽  
Vol 3 (1) ◽  
pp. 68-77 ◽  
Author(s):  
Evandro L. Dall'Oglio ◽  
Paulo T. de Sousa ◽  
Leonardo Gomes de Vasconcelos ◽  
Carlos Adriano Parizotto ◽  
Ewerton Ferreira Barros ◽  
...  
Keyword(s):  
Continuous Flow ◽  
Nir Spectroscopy ◽  
Flow Cell ◽  
Biodiesel Production ◽  
Microwave Assisted ◽  
Acid Catalyzed

Continuous-flow, well-mixed, microfluidic crystallization device for screening of polymorphs, morphology, and crystallization kinetics at controlled supersaturation

Lab on a Chip ◽  
2019 ◽  
Vol 19 (14) ◽  
pp. 2373-2382 ◽  
Author(s):  
Paria Coliaie ◽  
Manish S. Kelkar ◽  
Nandkishor K. Nere ◽  
Meenesh R. Singh
Keyword(s):  
Crystallization Kinetics ◽  
Microfluidic Device ◽  
Continuous Flow ◽  
Crystalline Materials ◽  
Screening Techniques

While the conventional screening techniques suffer from depletion of supersaturation, the continuous-flow microfluidic device screens crystalline materials at controlled supersaturation.

scholarly journals Effects of Hydrocortisone on the Yield and Bactericidal Function of Granulocytes Collected by Continuous-flow Centrifugation

Blood ◽  
1974 ◽  
Vol 44 (3) ◽  
pp. 435-443 ◽  
Author(s):  
Mamoru Shoji ◽  
William R. Vogler
Keyword(s):  
Single Dose ◽  
Flow Rate ◽  
Continuous Flow ◽  
Treated Group ◽  
Flow Cell ◽  
Controlled Study ◽  
Single Intravenous Injection ◽  
Cell Separator ◽  
Bactericidal Function

Abstract The usefulness of granulocyte transfusions is in part dependent upon the number of granulocytes transfused. The invention of the continuous-flow cell separator has made it possible to obtain granulocytes from normal donors. Efforts to improve the yield are under study. This controlled study was undertaken to determine the effect of a single dose of hydrocortisone on granulocyte yield from volunteer donors and on granulocyte bactericidal function. Twenty-two normal volunteers were randomized between no therapy or a single intravenous injection of 120 mg/sq m of hydrocortisone 2 hr prior to initiation of a 4-hr leukapheresis using the Aminco cell separator operated at 750 rpm and a flow rate of 41 ml/min. Significant increases in granulocyte yield and reductions in lymphocyte and monocyte yields were obtained in the hydrocortisone-treated group. Granulocytes from each group were equally effective in the phagocytosis of yeast particles and in vitro bactericidal activity.

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