Boron Nitride Nanotubes Reinforce Tricalcium Phosphate Scaffolds and Promote the Osteogenic Differentiation of Mesenchymal Stem Cells

2016 ◽  
Vol 12 (5) ◽  
pp. 934-947 ◽  
Author(s):  
Cijun Shuai ◽  
Chengde Gao ◽  
Pei Feng ◽  
Tao Xiao ◽  
Kun Yu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Boron Nitride ◽  
Osteogenic Differentiation ◽  
Tricalcium Phosphate ◽  
Boron Nitride Nanotubes

Boron nitride nanotube-enhanced osteogenic differentiation of mesenchymal stem cells

2015 ◽  
Vol 104 (2) ◽  
pp. 323-329 ◽  
Author(s):  
Xia Li ◽  
Xiupeng Wang ◽  
Xiangfen Jiang ◽  
Maho Yamaguchi ◽  
Atsuo Ito ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Boron Nitride ◽  
Osteogenic Differentiation ◽  
Boron Nitride Nanotube

Evaluation of the effects of boron nitride nanotubes functionalized with gum arabic on the differentiation of rat mesenchymal stem cells

RSC Advances ◽  
2015 ◽  
Vol 5 (56) ◽  
pp. 45431-45438 ◽  
Author(s):  
Tiago H. Ferreira ◽  
Antonella Rocca ◽  
Attilio Marino ◽  
Virgilio Mattoli ◽  
Edesia M. B. de Sousa ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Proliferation ◽  
Mesenchymal Stem Cells ◽  
Boron Nitride ◽  
Gum Arabic ◽  
Boron Nitride Nanotubes ◽  
Cell Proliferation And Differentiation ◽  
Proliferation And Differentiation

The biocompatibility of boron nitride nanotubes with rat mesenchymal stem cells has been evaluated in terms of cell proliferation and differentiation.

Beta-tricalcium phosphate promotes osteogenic differentiation of bone marrow-derived mesenchymal stem cells through macrophages

2021 ◽  
Vol 16 (2) ◽  
pp. 025005
Author(s):  
Mengting Zheng ◽  
Mengjia Weng ◽  
Xiaoyu Zhang ◽  
Ruomei Li ◽  
Qian Tong ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Osteogenic Differentiation ◽  
Tricalcium Phosphate ◽  
Beta Tricalcium Phosphate

scholarly journals Modifications in Gene Expression in the Process of Osteoblastic Differentiation of Multipotent Bone Marrow-Derived Human Mesenchymal Stem Cells Induced by a Novel Osteoinductive Porous Medical-Grade 3D-Printed Poly(ε-caprolactone)/β-tricalcium Phosphate Composite

2021 ◽  
Vol 22 (20) ◽  
pp. 11216
Author(s):  
Ivan López-González ◽  
Camilo Zamora-Ledezma ◽  
María Isabel Sanchez-Lorencio ◽  
Elena Tristante Barrenechea ◽  
José Antonio Gabaldón-Hernández ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Osteogenic Differentiation ◽  
Tricalcium Phosphate ◽  
Osteoblastic Differentiation ◽  
Alizarin Red ◽  
3D Printed ◽  
Medical Grade

In this work, we evaluated the influence of a novel hybrid 3D-printed porous composite scaffold based on poly(ε-caprolactone) (PCL) and β-tricalcium phosphate (β-TCP) microparticles in the process of adhesion, proliferation, and osteoblastic differentiation of multipotent adult human bone marrow mesenchymal stem cells (ah-BM-MSCs) cultured under basal and osteogenic conditions. The in vitro biological response of ah-BM-MSCs seeded on the scaffolds was evaluated in terms of cytotoxicity, adhesion, and proliferation (AlamarBlue Assay®) after 1, 3, 7, and 14 days of culture. The osteogenic differentiation was assessed by alkaline phosphatase (ALP) activity, mineralization (Alizarin Red Solution, ARS), expression of surface markers (CD73, CD90, and CD105), and reverse transcription–quantitative polymerase chain reaction (qRT-PCR) after 7 and 14 days of culture. The scaffolds tested were found to be bioactive and biocompatible, as demonstrated by their effects on cytotoxicity (viability) and extracellular matrix production. The mineralization and ALP assays revealed that osteogenic differentiation increased in the presence of PCL/β-TCP scaffolds. The latter was also confirmed by the gene expression levels of the proteins involved in the ossification process. Our results suggest that similar bio-inspired hybrid composite materials would be excellent candidates for osteoinductive and osteogenic medical-grade scaffolds to support cell proliferation and differentiation for tissue engineering, which warrants future in vivo research.

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