Evaluation of the effects of boron nitride nanotubes functionalized with gum arabic on the differentiation of rat mesenchymal stem cells

RSC Advances ◽  
2015 ◽  
Vol 5 (56) ◽  
pp. 45431-45438 ◽  
Author(s):  
Tiago H. Ferreira ◽  
Antonella Rocca ◽  
Attilio Marino ◽  
Virgilio Mattoli ◽  
Edesia M. B. de Sousa ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Proliferation ◽  
Mesenchymal Stem Cells ◽  
Boron Nitride ◽  
Gum Arabic ◽  
Boron Nitride Nanotubes ◽  
Cell Proliferation And Differentiation ◽  
Proliferation And Differentiation

The biocompatibility of boron nitride nanotubes with rat mesenchymal stem cells has been evaluated in terms of cell proliferation and differentiation.

Umbilical cord mesenchymal stem cells suppress B-cell proliferation and differentiation

2012 ◽  
Vol 274 (1-2) ◽  
pp. 46-53 ◽  
Author(s):  
Nan Che ◽  
Xia Li ◽  
Shiliang Zhou ◽  
Rui Liu ◽  
Dongyan Shi ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Proliferation ◽  
Mesenchymal Stem Cells ◽  
B Cell ◽  
Umbilical Cord ◽  
Cell Proliferation And Differentiation ◽  
Proliferation And Differentiation

scholarly journals Bone Marrow Mesenchymal Stem Cells Stimulate Cardiac Stem Cell Proliferation and Differentiation

2010 ◽  
Vol 107 (7) ◽  
pp. 913-922 ◽  
Author(s):  
Konstantinos E. Hatzistergos ◽  
Henry Quevedo ◽  
Behzad N. Oskouei ◽  
Qinghua Hu ◽  
Gary S. Feigenbaum ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Cell Proliferation ◽  
Mesenchymal Stem Cells ◽  
Stem Cell ◽  
Cardiac Stem Cell ◽  
Stem Cell Proliferation ◽  
Cell Proliferation And Differentiation ◽  
Proliferation And Differentiation ◽  
Marrow Mesenchymal Stem Cells

Cyclic ADP-Ribose Increases Human Mesenchymal Stem Cells Proliferation through the Modulation of Spontaneous Ca2+ Oscillation.

Blood ◽  
2007 ◽  
Vol 110 (11) ◽  
pp. 4061-4061
Author(s):  
Rong Tao ◽  
Chu-Pak Lau ◽  
Hon-Cheung Lee ◽  
Gui-Rong Li
Keyword(s):  
Stem Cells ◽  
Cell Proliferation ◽  
Mesenchymal Stem Cells ◽  
Ryanodine Receptors ◽  
Human Mesenchymal Stem Cells ◽  
Hematopoietic Stem ◽  
Cell Proliferation And Differentiation ◽  
Proliferation And Differentiation ◽  
Cyclic Adp Ribose ◽  
Trpm2 Channels

Abstract Human mesenchymal stem cells (hMSCs) play an important role in the regulation of hematopoietic microenvironment. Cyclic ADP-ribose (cADPR), a universal Ca2+ mobilizer produced by hMSCs in the bone marrow, has been demonstrated to be an important factor in the regulation of hematopoietic stem cells proliferation. However, its biological effects on hMSCs are not understood. The present study was designed to investigate the effects of cADPR on Ca2+ signaling, cell proliferation and differentiation in hMSCs. Ca2+ activity in hMSCs was measured with a confocal microscope. It was found that cADPR increased the frequency of spontaneous Ca2+ oscillatory transient, and the effect was abolished by pretreatment with the specific cADPR antagonist 8-Br-cADPR, but not by ryanodine, though the ryanodine receptors are the classic targets of cADPR in other types of cells. RT-PCR showed no gene expression for ryanodine receptors in hMSCs. Interestingly, the cADPR-induced increase of spontaneous Ca2+ oscillation was abolished by the TRPM2 channel inhibitors econazole and clotrimazole. The downregulation of TRPM2 channels with siRNA technique had no significant effects on spontaneous Ca2+ oscillation; however, it abrogated the cADPR-induced increase of spontaneous Ca2+ oscillation. The effects of cADPR on cell proliferation and differentiation were then investigated in hMSCs. We found that cADPR increased hMSCs proliferation, but had no significant effects on osteogeneic and adipogeneic differentiation. Importantly, the effect of cADPR on hMSCs proliferation was antagonized by 8-Br-cADPR or by the selectively knockdown of TRPM2 channels. In conclusion, our results demonstrate the novel information that cADPR regulate cell proliferation by increasing spontaneous Ca2+ oscillation mediated by TRPM2 channels, but has no effects on osteogeneic and adipogeneic differentiation in hMSCs.

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