Locomotor Activity in Drosophila Melanogaster

1984 ◽  
Vol 35 (3) ◽  
pp. 438-454 ◽  
Author(s):  
W. Scharloo ◽  
H. Van Stolwijk ◽  
F.R. Van Dijken
Keyword(s):  
Drosophila Melanogaster ◽  
Locomotor Activity ◽  
Experimental Conditions ◽  
Ether Anaesthesia ◽  
Locomotory Behaviour ◽  
Culturing Conditions ◽  
Adult Drosophila ◽  
Field Behaviour ◽  
Age And Sex ◽  
Open Field Behaviour

The effects of various experimental conditions on locomotor activity of adult Drosophila melanogaster flies of both sexes were measured in the locometer. This apparatus measures linear progression of flies and is based on the fractionating principle of mazes used for behavioural studies. Groups of approximately 100 flies are tested simultaneously but every individual fly is assigned a locomotor activity score. This score is positively correlated with open-field behaviour as measured in the Elens-Wattiaux-chamber. Strong lasting effects of ether anaesthesia were demonstrated. Other anaesthetics (c.q. CO 2 and N 2 ) did not show such profound effects. The effects of culturing conditions, density, age and sex were measured. The locometer proved to be useful for the investigations of the effects of different stimuli on the locomotory behaviour of fruitflies.

EFFECTS OF INGESTED ETHANOL ON ADULT DROSOPHILA MELANOGASTER (DIPTERA: DROSOPHILIDAE)

1981 ◽  
Vol 113 (4) ◽  
pp. 315-323 ◽  
Author(s):  
S. S. Chawla ◽  
J.-M. Perron ◽  
C. Radouco-Thomas
Keyword(s):  
Drosophila Melanogaster ◽  
Experimental Conditions ◽  
Water Solutions ◽  
Sucrose Solutions ◽  
Adult Drosophila ◽  
Better Than

AbstractAn apparatus consisting of a plastic box and a feeding circuit permits the calculation of μl volumes of ethanol ingested by Drosophila melanogaster Meigen. The net amount of ethanol taken (expressed in 100% ethanol) has been examined in various experimental conditions: sucrose solutions, ethanol–sucrose solutions at various sucrose and alcohol concentrations, ethanol solutions - cube of sucrose and ethanol solutions alone. The data obtained on ingestion by Drosophila show that the consumption of sucrose solutions was inversely proportional to the concentrations of sucrose. The amount of ethanol ingested during 14 days was directly related to the concentration of ethanol in the diets although in general the total volume of solution consumed did not vary significantly in most cases. The analyses of observations of 14 days showed that decreased mortality was obtained with the rise in the concentration of sucrose in sucrose-water solutions in 0.5–3% range and the mortality increased with the rise in the concentration of ethanol in the diets, yet ethanol-sucrose was better than cube of sugar alone.

Parental and preimaginal exposure to methylmercury disrupts locomotor activity and circadian rhythm of adult Drosophila melanogaster

2018 ◽  
Vol 43 (3) ◽  
pp. 255-265 ◽  
Author(s):  
Thaís Doeler Algarve ◽  
Charles Elias Assmann ◽  
Toshiro Aigaki ◽  
Ivana Beatrice Mânica da Cruz
Keyword(s):  
Drosophila Melanogaster ◽  
Circadian Rhythm ◽  
Locomotor Activity ◽  
Adult Drosophila

Genetic analysis of oxygen defense mechanisms in Drosophila melanogaster and identification of a novel behavioural mutant with a Shaker phenotype

Genome ◽  
1996 ◽  
Vol 39 (4) ◽  
pp. 749-757 ◽  
Author(s):  
James M. Humphreys ◽  
Brenda Duyf ◽  
Mei-Ling A. Joiner ◽  
John P. Phillips ◽  
Arthur J. Hilliker
Keyword(s):  
Drosophila Melanogaster ◽  
Defense Mechanisms ◽  
Chromosome Region ◽  
Recessive Mutation ◽  
Ether Anaesthesia ◽  
Ros Metabolism ◽  
Chromosome Mutations ◽  
New Gene ◽  
New Alleles

Mutants of Drosophila melanogaster that lack Cu/Zn superoxide dismutase or urate are hypersensitive to reactive oxygen species (ROS) generated in vivo by the redox-cycling agent paraquat. We have subsequently employed paraquat as a selective agent to identify adult viable mutants potentially defective in other, perhaps unknown, components of ROS metabolism. Paraquat screening of ethyl methanesulfonate-induced second- and third-chromosome mutations yielded 24 paraquat hypersensitive mutants. Two mutants were identified as being new alleles of the previously identified doublesex (dsx) and pink (p) genes. The remainder of the mutations identified previously undescribed genes, including one second chromosome paraquat hypersensitive mutant that was found to exhibit shaking legs, abdomen pulsations, and body shuddering under ether anaesthesia. This recessive mutation was mapped to the polytene chromosome region of 48A5–48B2 and defines a new gene we named quiver (qvr). This mutation is similar in phenotype to the Shaker (Sh), ether-a-gogo (eag), and Hyperkinetic (Hk) mutations, all of which affect potassium channel function in D. melanogaster. Key words : Drosophila, paraquat, EMS-mutagenesis, Shaker, oxidative-stress.

Neuropeptide F immunoreactive clock neurons modify evening locomotor activity and free-running period in Drosophila melanogaster

2012 ◽  
Vol 520 (5) ◽  
pp. 970-987 ◽  
Author(s):  
Christiane Hermann ◽  
Taishi Yoshii ◽  
Verena Dusik ◽  
Charlotte Helfrich-Förster
Keyword(s):  
Drosophila Melanogaster ◽  
Locomotor Activity ◽  
Free Running ◽  
Neuropeptide F ◽  
Free Running Period
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