scholarly journals Raman Spectrum-Based Diagnosis Strategy for Bladder Tumor

2021 ◽  
pp. 1-7
Author(s):  
Zhenghong Liu ◽  
Pu Zhang ◽  
Heng Wang ◽  
Bin Zheng ◽  
Li Sun ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Bladder Tumor ◽  
Large Population ◽  
Review Article ◽  
Optical Technique ◽  
Bladder Tumors ◽  
Clinical Value ◽  
Tissue Sections ◽  
Diagnosis Method ◽  
Endoscopic Methods

Raman spectroscopy is an optical technique that can potentially serve as a molecular diagnosis method. This approach is excellent in many aspects for diagnosing bladder tumors, and over the last 20 years, there has been a rapid increase in the number of related studies. However, no review article has covered the wide use of Raman spectroscopy in bladder tumors. A total of 26 original studies have suggested that Raman spectroscopy shows good performance in diagnosing bladder tumors from 4 aspects, including tissue sections, endoscopic methods, cell screening, and biomarkers. However, Raman spectroscopy needs to be modified by combining it with other techniques, and studies based on a large population are still urgently needed to expand its clinical value.

Comparison of Endoscopic Versus Percutaneous Approach (Blind) to Control the Obturator Jerk in Patients Undergoing Transurethral Resection of Bladder Tumors Under Spinal Anesthesia

2020 ◽  
Vol 21 (2) ◽  
pp. 120-126
Author(s):  
Alamgir Md ◽  
Karim Km Monwarul ◽  
Nandy SP ◽  
Md Monwar Ul Haque ◽  
Sakhawat Mahmud Khan
Keyword(s):  
Statistical Analysis ◽  
Spinal Anesthesia ◽  
Transurethral Resection ◽  
Bladder Tumor ◽  
Complete Resection ◽  
Satisfaction Level ◽  
Bladder Tumors ◽  
Percutaneous Approach ◽  
Group A ◽  
Group B

Objective: The aim of the study was to compare the endoscopic versus percutaneous approach (blind) to control the obturator jerk in patients undergoing transurethral resection of bladder tumors under spinal anesthesia. Materials and methods: A prospective observational study was performed in Department of Urology, Chittagong Medical College, Chittagong and some Private Hospitals (Ltd.) in Chittagong city during the period from January 2016 to June 2016. Total 100 patients were grouped into two, on alternate basis. Fifty(50) patients in group- A conducted with endoscopic infiltration with 20ml of injection 2% lignocaine at the bladder tumor base and another 50 patients in group-B, conducted with blind percutaneous technique with same drug and volume ( 20ml inj.2% lignocaine) to control obturator jerk. Severity of obturator jerk in both procedure, percentage of complete resection, ONB procedure related time, ONB procedure related complications and surgeon’s satisfaction level were recorded and compared between two approaches. Chi-square analysis was performed to compare the ease of approach and outcome of the two techniques. A value of P<0.05 was considered statistically significant. Results: The mean age of the patients were 59.44+7.681. In group-A, 50 patients were given inj. 2% lignocaine endoscopically at the bladder tumor base to control obturator jerk. Twenty five patients (50%) had no jerk, 20 patients(40%) developed mild jerk and 5 patients (10%) developed moderate jerk and no patients developed severe jerk. Second attempt was taken in moderate jerk patients (5 patients) and succeeded in 3(6%) patients. So, in this group, complete resection of bladder tumor was possible in 96%. In group B, complete resection of bladder tumor was possible in 84%. Statistical analysis was done and result is significant in case of endoscopic procedure to control obturator jerk(p<0.05). ONB Procedure related time was <20 mins. in 32(64%) patients in group-A and 45 (90%) patients in group- B. 20 mins. or more time was required for 18 (36%) patients in group-A and 5 (10%) patients in group-B. Statistical analysis was done and result is significant in percutaneous (blind) technique (p<0.05). ONB procedure related complications in group-A and Group –B were noted. Statistical analysis was done and result is insignificant (p>0.05). Surgeons satisfaction level were recorded on the basis of obturator jerk block and complete resection and which was statistically significant in favour of endoscopy group (p<0.05). Conclusion: It is concluded that endoscopic injection of 2% lignocaine into the bladder tumor base is better in case of jerk elimination and complete resection than blind percutaneous approach. Though, ONB procedure related time was significantly less in percutaneous group. Bangladesh Journal of Urology, Vol. 21, No. 2, July 2018 p.120-126

scholarly journals Group Testing-Based Robust Algorithm for Diagnosis of COVID-19

Diagnostics ◽  
2020 ◽  
Vol 10 (6) ◽  
pp. 396
Author(s):  
Jin-Taek Seong
Keyword(s):  
Large Population ◽  
Group Testing ◽  
Incidence Rates ◽  
Posteriori Probability ◽  
Iterative Detection ◽  
Robust Algorithm ◽  
A Posteriori Probability ◽  
Small Set ◽  
Diagnosis Method ◽  
Severe Infections

At the time of writing, the COVID-19 infection is spreading rapidly. Currently, there is no vaccine or treatment, and researchers around the world are attempting to fight the infection. In this paper, we consider a diagnosis method for COVID-19, which is characterized by a very rapid rate of infection and is widespread. A possible method for avoiding severe infections is to stop the spread of the infection in advance by the prompt and accurate diagnosis of COVID-19. To this end, we exploit a group testing (GT) scheme, which is used to find a small set of confirmed cases out of a large population. For the accurate detection of false positives and negatives, we propose a robust algorithm (RA) based on the maximum a posteriori probability (MAP). The key idea of the proposed RA is to exploit iterative detection to propagate beliefs to neighbor nodes by exchanging marginal probabilities between input and output nodes. As a result, we show that our proposed RA provides the benefit of being robust against noise in the GT schemes. In addition, we demonstrate the performance of our proposal with a number of tests and successfully find a set of infected samples in both noiseless and noisy GT schemes with different COVID-19 incidence rates.

scholarly journals Inflammatory Myofibroblastic Bladder Tumor in a Patient with Von Recklinghausen's Syndrome

2010 ◽  
Vol 2010 ◽  
pp. 1-4 ◽  
Author(s):  
Eleftherios Chatzidarellis ◽  
Evangelos Mazaris ◽  
Andreas Skolarikos ◽  
Demonakou Maria ◽  
Iraklis Mitsogiannis ◽  
...  
Keyword(s):  
Transurethral Resection ◽  
Inflammatory Myofibroblastic Tumor ◽  
Bladder Tumor ◽  
Inflammatory Pseudotumor ◽  
Benign Tumor ◽  
Pathologic Examination ◽  
Bladder Tumors ◽  
Macroscopic Hematuria ◽  
History Of ◽  
Suprapubic Prostatectomy

Myofibroblastic tumor, also known as inflammatory pseudotumor or pseudosarcoma, is a benign tumor with mesenchymal origin. Bladder location is very uncommon. We report the case of a 58-year-old man with a history of von Recklinghausen's disease who complained for painless macroscopic hematuria 5 months after suprapubic prostatectomy. The radiograph evaluation revealed a bladder tumor, and the pathologic examination following a transurethral resection showed inflammatory myofibroblastic tumor of the bladder. The patient finally underwent a radical cystectomy due to the uncertain pathogenesis of inflammatory myofibroblastic tumor as well as the rarity of cases published on bladder tumors in Von Recklinghausen's patients.

Neoadjuvant chemotherapy and bladder-sparing surgery for invasive bladder cancer: ten-year outcome.

1998 ◽  
Vol 16 (4) ◽  
pp. 1298-1301 ◽  
Author(s):  
H W Herr ◽  
D F Bajorin ◽  
H I Scher
Keyword(s):  
Bladder Cancer ◽  
Radical Cystectomy ◽  
Bladder Tumor ◽  
Tumor Node Metastasis ◽  
Bladder Tumors ◽  
Primary Tumor Site ◽  
Node Metastasis ◽  
Bladder Sparing ◽  
Salvage Cystectomy

PURPOSE To evaluate the 10-year outcome of patients with invasive (T2-3N0M0, staged according to the tumor, node, metastasis system) bladder cancer who responded completely to a combination of methotrexate, vinblastine, adriamycin, and cisplatin (MVAC) chemotherapy followed by bladder-sparing surgery. PATIENTS AND METHODS Of 111 surgical candidates who received neoadjuvant MVAC, 60 (54%) achieved a complete clinical response (T0) on transurethral resection (TUR) of the primary tumor site. Of these, 28 requested follow-up with TUR alone, 15 had a partial cystectomy, and 17 elected a radical cystectomy. The patients were followed up for a median of 10 years (range, 8 to 13 years). RESULTS Of 43 patients who had bladder-sparing surgery, 32 (74%) are alive, which includes 25 (58%) with an intact functioning bladder. Twenty-four patients (56%) developed bladder tumor recurrences from 5 to 96 months, which were invasive in 13 (30%) and superficial in 11 (26%). Thirteen patients required a salvage cystectomy, of whom 6 died, which includes 4 (9%) from a new invasive neoplasm. Of the 17 patients who had radical cystectomy, 11 (65%) are alive. CONCLUSION The majority of patients with invasive bladder tumors who achieve T0 status after neoadjuvant MVAC chemotherapy preserve their bladders for up to 10 years with bladder-sparing surgery. The bladder remains at risk for new invasive tumors. Cystectomy salvages the majority, but not all, of relapsing patients.

scholarly journals Can CT Virtual Cystoscopy Replace Conventional Cystoscopy in Early Detection of Bladder Cancer?

2015 ◽  
Vol 2015 ◽  
pp. 1-6 ◽  
Author(s):  
Sachin Abrol ◽  
Ankush Jairath ◽  
Sanika Ganpule ◽  
Arvind Ganpule ◽  
Shashikant Mishra ◽  
...  
Keyword(s):  
Bladder Cancer ◽  
Early Detection ◽  
Predictive Value ◽  
Bladder Tumor ◽  
Urothelial Cancer ◽  
Routine Clinical Practice ◽  
Bladder Tumors ◽  
Virtual Cystoscopy ◽  
Sensitivity Specificity

Aim. To correlate findings of conventional cystoscopy with CT virtual cystoscopy (CTVC) in detecting bladder tumors and to evaluate accuracy of virtual cystoscopy in early detection of bladder cancer.Material and Method. From June 2013 to June 2014, 50 patients (46 males, four females) with history and investigations suggestive of urothelial cancer, with mean age 62.76 ± 10.45 years, underwent CTVC by a radiologist as per protocol and subsequently underwent conventional cystoscopy (CPE) the same day or the next day. One urologist and one radiologist, blinded to the findings of conventional cystoscopy, independently interpreted the images, and any discrepant readings were resolved with consensus.Result. CTVC detected 23 out of 25 patients with bladder tumor(s) correctly. Two patients were falsely detected as negative while two were falsely labeled as positive in CTVC. Virtual and conventional cystoscopy were comparable in detection of tumor growth in urinary bladder. The sensitivity, specificity, positive predictive value, and negative predictive value of virtual cystoscopy were 92% each.Conclusion. CTVC correlates closely with the findings of conventional cystoscopy. Bladder should be adequately distended and devoid of urine at the time of procedure. However, more studies are required to define the role of virtual cystoscopy in routine clinical practice.

scholarly journals Bladder Tumor in Women with Microscopic Hematuria: An Iranian Experience and a Review of the Literature

2009 ◽  
Vol 2009 ◽  
pp. 1-5 ◽  
Author(s):  
Shahin Abbaszadeh ◽  
Saeed Taheri ◽  
Mohammad Hossein Nourbala
Keyword(s):  
Urge Incontinence ◽  
Bladder Tumor ◽  
Malignant Tumors ◽  
Bladder Wall ◽  
Microscopic Hematuria ◽  
Urinary Stones ◽  
Bladder Tumors ◽  
Presenting Symptoms ◽  
Study Population ◽  
Tract Infections

Aim. In this study we report our experience with microhematuria and its relation with bladder tumors in Iranian women.Materials and Methods. Overall 249 women were evaluated. Microscopic hematuria was defined as three or more red blood cells per high-power field on at least two different occasions. Patients with a history of gross hematuria or coagulation disorders, having organic diseases, urinary stones, urinary tract infections, nephrological diseases, and local lesions such as urethral caruncle were excluded from the study population. Final diagnosis of malignant tumors was done with cystoscopy and biopsy specimen pathological assessment in all cases.Results. Age for the study population was49.7±11.8years. 95 (38%) of patients were identified during routine check up and presenting symptoms in other patients were frequency, dysuria, stress urge incontinence, urge incontinence, feeling of incomplete urine emptying, and flunk pain, respectively. Finally, 7 (2.8%) of study subjects were confirmed as having bladder tumors. One of tumor cases was diagnosed 24 months after initial assessments. Patients with bladder tumor were significantly older; more frequently had diverticulum in their bladder wall (P<.05).Conclusion. Female microscopic hematuria is relevant and deserves evaluations, especially in elderly patients. Patients whose reason for microhematuria would not be diagnosed at the initial evaluations should be followed.

Endoscopic Snare Resection of Bladder Tumors: Evaluation of an Alternative Technique for Bladder Tumor Resection*

2012 ◽  
Vol 26 (6) ◽  
pp. 614-617 ◽  
Author(s):  
Matthew J. Maurice ◽  
Gino J. Vricella ◽  
Gregory MacLennan ◽  
Peter Buehner ◽  
Lee E. Ponsky
Keyword(s):  
Bladder Tumor ◽  
Tumor Resection ◽  
Alternative Technique ◽  
Bladder Tumors

scholarly journals A Novel CpG Oligodeoxynucleotide Enhanced the Therapeutic Effect of Epirubicin on Bladder Tumors in Rats

2021 ◽  
Author(s):  
Yang Luo ◽  
Xiaoyi Fu ◽  
Bin Dong ◽  
Hongsheng Men ◽  
Shulin Zhang ◽  
...  
Keyword(s):  
Therapeutic Effect ◽  
Rat Model ◽  
Bladder Tumor ◽  
Interleukin 2 ◽  
P53 Gene ◽  
Clinical Effects ◽  
Bacillus Calmette Guerin ◽  
Bladder Tumors ◽  
Cpg Oligodeoxynucleotides ◽  
Cpg Oligodeoxynucleotide

Abstract Background CpG oligodeoxynucleotides, which boast anti-inflammatory, anti-infectious, and chemotherapeutic activities, are promising immunomodulators. Recently, some preclinical studies have highlighted the potent immunostimulatory and anti-tumor effects of CpG oligodeoxynucleotides, which has aroused interest in their potential clinical effects on human cancers. Methods In this study, we evaluated the therapeutic effect of a new type of CpG oligodeoxynucleotide whose sequence (5’-AACGTTGTCGTCGACGTCGTCGTCAGGCCTGACGTTATCGATGGCGTTGTCGTCAACGTTGTCGTTAACGTT-3’) was designed by our laboratory in combination with epirubicin in a bladder tumor rat model induced by N-methyl-N-nitrosourea instillation. Moreover, we explored the safety of the novel CpG oligodeoxynucleotide for bladder tumor therapy by observing the degree of cystolith in the bladder and comparing the results against those of Bacillus Calmette–Guérin therapy, which is a gold-standard treatment for bladder tumor. Results All results showed that CpG oligodeoxynucleotide combined with epirubicin significantly inhibited the growth of bladder tumors and reduced the pathological grading. As compared with bladder cells or cytokines observed under the positive control or epirubicin-alone treatment conditions, all indexes including histopathological grading, Mutation P53 gene protein expression, and Interleukin-2 (IL-2) level were significantly optimized by instillation of CpG oligodeoxynucleotide. Immunohistochemical examination indicated that CpG oligodeoxynucleotide reduced the expression of Mutation P53 gene protein in the bladder tumor rat model. Specifically, the level of IL-2 in rat serum was increased by more than 30% CpG oligodeoxynucleotide treatment combined with epirubicin. Also, in comparison with the degree of cystolith observed in the Bacillus Calmette–Guérin group, no obvious side effects were caused by CpG oligodeoxynucleotide. Conclusions CpG oligodeoxynucleotide as an immunomodulator can enhance the efficacy of epirubicin and presents higher safety than Bacillus Calmette–Guérin in treating bladder cancer.

scholarly journals COVID-19 Vaccines in Pakistan: Efficacy, Adverse Effects and Availability

2021 ◽  
Vol 10 (2) ◽  
pp. 125-130
Author(s):  
Soma Siddique ◽  
Shaheer Ahmed
Keyword(s):  
Clinical Trials ◽  
Side Effects ◽  
Adverse Effects ◽  
Mode Of Action ◽  
Public Awareness ◽  
Grey Literature ◽  
Large Population ◽  
Review Article ◽  
Pain On Injection ◽  
The World

In this review article, we aim to document the efficacy, adverse effects, mode of action, required doses, and availability of the major vaccines available in Pakistan till 20 May 2021. We reviewed all available literature on COVID-19 vaccines in PubMed and Google scholar. We also reviewed articles from grey literature. Currently, Pfizer–BioNTech and Moderna, Sinopharm (China), Sputnik V (Russia), CoronaVac (popularly known as Sinovac) (China), Cansino, and Vaxzevria vaccines have been authorized for emergency use in several countries. Pakistan has sanctioned the use of all the aforementioned vaccines except Pfizer and Moderna. As per their efficacy, Pfizer and Moderna have been found most effective among all the vaccines with 95% effectiveness, while the Vaxzevria, Sputnik V, Sinopharm, and Cansino have shown 70%, 91.6%, 79.34%, and 90% effectiveness, respectively. All the vaccines have shown milder side effects like headache, fever, and pain on injection sites. To curb the pandemic, more clinical trials are being conducted throughout the world. Importantly public awareness is warranted to achieve the target of vaccinating a large population.

Fast Characteristic of Skin Lesions by Machine-Learning of Raman Spectrum

2021 ◽  
Author(s):  
Hua Zhang ◽  
Danhua Wang ◽  
Limei Qu ◽  
Ying Xue ◽  
Xinli Li ◽  
...  
Keyword(s):  
Machine Learning ◽  
Raman Spectroscopy ◽  
Raman Spectrum ◽  
Skin Lesions ◽  
Support Vector ◽  
Learning Technology ◽  
K Nearest Neighbor ◽  
Seborrheic Keratosis ◽  
Inspection Method ◽  
Tissue Sections

Abstract Background: The traditional diagnosis of skin lesions mainly relies on dermoscope and pathological biopsy, of which the former is non-objective and the latter is invasive and time-consuming. It is necessary to find an objective and non-invasive inspection method for the diagnosis of skin cancer which is the most common malignant tumor. Herein, we aimed to fast identify the skin cancers on ultrathin frozen fresh tissue sections by combining Raman spectroscopy detection and machine learning technology. Methods and material: 22 fresh frozen tissue sections including 3 squamous cell carcinomas, 11 basal cell carcinomas, 2 malignant melanomas, 3 seborrheic keratosis, and 3 melanocytic nevi, were included and performed Raman detection. To prevent the discrete Raman data distribution affecting the generalization ability of the learning model, a series of adaptive preprocessing algorithms were first applied to standardize the raw Raman data of five skin lesions. The processed Raman data were performed visualized cluster analysis by principal components analysis (PCA) and t-distributed stochastic neighbor embedding (t-SNE). And, using K-nearest Neighbor (KNN) and support vector machine (SVM) classifiers, two predictive models for diagnose were established and evaluated in the training set and test set by the confusion matrixes and receiver operating characteristic (ROC) curves.Results: The mean variance Raman spectrum graph of 5 skin lesion types were acquired after standardization procession and 4 peak positions with large differences were found. Through dimensionality reduction by PCA and t-SNE, the visual clustering results of Raman data showed heterogeneous intra-cluster homogeneity and inter-cluster dispersion. The test accuracies reached 94.56% and 98.94% in KNN and SVM classifiers respectively. The areas under the ROCs of the two classifiers, in the category dimension and the sample dimension, were all more than 0.99 which is close to the perfect classification effect. Conclusions: Raman spectroscopy is a competitive candidate for the fast and accurate diagnosis of skin lesions and the molecular information provided may be used in the pathological classification, predicting immunotherapy responsiveness and stratifying prognostic risk. Furthermore, the combination of Raman spectroscopy and machine learning methods showed great diagnostic capabilities with high accuracy is a promising tool for the diagnosis of skin lesions.

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