Penile Lichen Sclerosus and “Nevus Elasticus”

2021 ◽  
pp. 1-3
Author(s):  
Maiara Ferreira de Souza ◽  
Wendel Souza Kruschewsky ◽  
Nathanael de Freitas Pinheiro Junior ◽  
Daniel Abensur Athanazio
Keyword(s):  
Blood Vessels ◽  
Cutaneous Lesion ◽  
Lichen Sclerosus ◽  
Elastic Fibers ◽  
Deep Dermis

The association between penile lichen sclerosus and striking accumulation of elastic fibers in deep dermis has been described in rare reports, mostly in vulvar lesions. We describe one case of severe balanopreputial adhesions related to lichen sclerosus and this form of elastosis, with no concomitant neoplasia. Aggregates of elastic fibers were seen in deep dermis and in blood vessels. The lesion mirrors nevus elasticus and nevus elasticus vascularis – a well described cutaneous lesion with no known association with lichen sclerosus.

Increase of elastic fibers in lichen sclerosus et atrophicus

2014 ◽  
Vol 41 (8) ◽  
pp. 646-649 ◽  
Author(s):  
Yohei Shiba ◽  
Koji Ono ◽  
Minoru Akiyama ◽  
Norihiro Fujimoto ◽  
Shingo Tajima
Keyword(s):  
Lichen Sclerosus ◽  
Elastic Fibers ◽  
Lichen Sclerosus Et Atrophicus

scholarly journals Effect and Repair Mechanism of Nano Ag Sponge Dressing Combined with Gelatin-Bletilla Striata Gum/Salvia Miltiorrhiza on Refractory Orthopedic Wounds

2021 ◽  
Vol 2021 ◽  
pp. 1-8
Author(s):  
Chuanjie Jiao ◽  
Ming Deng ◽  
Yonggang Ma ◽  
Geliang Hu
Keyword(s):  
Wound Healing ◽  
Blood Vessels ◽  
Healing Rate ◽  
Salvia Miltiorrhiza ◽  
Collagen Fibers ◽  
Hair Follicles ◽  
Elastic Fibers ◽  
Bletilla Striata ◽  
Ctrl Group ◽  
Tgf Β1

Objective. To explore the effect and mechanism of the sponge dressing on the healing of refractory orthopedic wound, and the gelatin-Bletilla striata gum/Salvia miltiorrhiza nano Ag (GBS-Ag) sponge dressing was prepared. Methods. GBS-Ag sponge dressing was prepared by the freeze-drying method. Twenty male SD rats were randomly divided into the control group (Ctrl group) and GBS-Ag group, with 10 rats in each group, and the rats in the two groups were established a model of back wound infection. The Ctrl group was treated with gauze, while the GBS-Ag group was treated with GBS-Ag sponge dressing. Wound healing rate, blood immune indexes, Ag content in each organ, morphological changes of wound, and expression of transforming growth factor-β1 (TGF-β1) in wound transformation were detected in the two groups of rats. Results. The mechanical properties of GBS-Ag sponge dressing were all in line with the standard, and it had good killing effect on the conventional strain after being incubated for 24 hours. Compared with the Ctrl group, the healing rate and lymphocyte percentage in the GBS-Ag group were significantly increased on day 4 and day 10 ( P < 0.05 ), while the total number of white blood cells and the percentage of neutrophils were significantly decreased ( P < 0.05 ). Compared with Ctrl group, the Ag content in liver, spleen, and kidney of rats in the GBS-Ag group was significantly increased ( P < 0.05 ). The histological results showed that the Ctrl group lacked collagen fibers in the dermis, and the angiogenesis was not rich, accompanied by a large number of inflammatory cell infiltration. The epidermal repair of rats in the GBS-Ag group was complete and partially keratinized, the dermis was rich in collagen fibers, with elastic fibers and new blood vessels, inflammatory cells were rare, and new hair follicles and thick-walled blood vessels were also observed. The expression of TGF-β1 protein in the wounds of rats in the GBS-Ag group was higher than that of the Ctrl group. Conclusion. GBS-Ag sponge dressing had multiple effects of sterilization and promoting wound healing, and its mechanism may be related to promoting the TGF-β1 protein expression.

scholarly journals Characterization of Collagens Fibers (I, III, IV) and Elastin in the Extracellular Matrix of Normal and Neoplastic Canine Prostate

2019 ◽  
Author(s):  
Luis Gabriel Rivera Calderón ◽  
Priscila Emiko Kobayashi ◽  
Rosemeri Oliveira Vasconcelos ◽  
Carlos Eduardo Fonseca-Alves ◽  
Renee Laufer-Amorim
Keyword(s):  
Extracellular Matrix ◽  
Smooth Muscle ◽  
Blood Vessels ◽  
Basal Membrane ◽  
Elastic Fibers ◽  
Normal Prostate ◽  
Type Iv ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Imagej Software

Collagen (Coll) is the most common protein in the extracellular matrix, responsible for providing tissue structure and support. In some types of cancer, including prostate cancer (PC) abundant collagen was identified and related to tumor progression and metastasis. This study aimed to investigate Coll-I, III, IV and elastin in normal canine prostatic tissue and PC, using the Picrosirius red (PSR) and Immunohistochemical (IHC) analysis. Eight normal prostates and 10 PC from formalin-fixed, paraffin-embedded samples were used. Collagen fibers area was analyzed with ImageJ software. The distribution of Coll-I and Col-III was approximately 80% around prostatic ducts and acini, 15% among smooth muscle and 5% around in blood vessels, in both normal prostate and PC. Immunostaining for type IV collagen was observed in the basal membrane of prostate acini, smooth muscle, blood vessels, and never fibers of normal and PC samples. Elastic fibers were found in the septa dividing the lobules and around the prostatic acini of normal samples. A high amount of elastic fibers was observed around the ducts and the urethra in normal and canine PC. The distribution and area percentage of staining for collagen are similar in normal and neoplastic canine prostate when analyzed with PSR and IHC.

scholarly journals Characterization of Collagens Fibers (I, III, IV) and Elastin of Normal and Neoplastic Canine Prostatic Tissues

2019 ◽  
Author(s):  
Luis Gabriel Rivera Calderón ◽  
Priscila Emiko Kobayashi ◽  
Rosemeri Oliveira Vasconcelos ◽  
Carlos Eduardo Fonseca-Alves ◽  
Renee Laufer-Amorim
Keyword(s):  
Smooth Muscle ◽  
Blood Vessels ◽  
Normal Tissue ◽  
Basal Membrane ◽  
Nerve Fibers ◽  
Elastic Fibers ◽  
Normal Prostate ◽  
Formalin Fixed Paraffin Embedded ◽  
Median Area ◽  
Imagej Software

This study aimed to investigate Coll-I, III, IV and elastin in canine normal prostate and PC, using Picrosirius red (PSR) and Immunohistochemical (IHC) analysis. Eight normal prostates and 10 PC from formalin-fixed, paraffin-embedded samples were used. Collagen fibers area was analyzed with ImageJ software. The distribution of Coll-I and Coll-III was approximately 80% around prostatic ducts and acini, 15% among smooth muscle and 5% surrounding blood vessels, in both normal prostate and PC. There was a higher median area of Coll-III in PC, when compared to normal prostatic tissue (p=0.001 for PSR and p= 0.05 for IHC). Immunostaining for Coll-IV was observed in the basal membrane of prostate acini, smooth muscle, blood vessels, and nerve fibers of normal and PC samples. Although there was no difference in Coll-IV area between normal tissue and PC, tumors with Gleason score 10 showed absence of Coll-IV, when compared to scores 6 and 8 (p=0.0095). Elastic fibers were found in the septa dividing the lobules and around the prostatic acini of normal samples, and was statistically higher in PC, compared to normal tissue (p=0.00229). Investigation of ECM components brings new information and should be correlated with prognosis in future studies.

scholarly journals Characterization of Collagens Fibers (I, III, IV) and Elastin in the Extracellular Matrix of Normal and Neoplastic Canine Prostatic Tissues

2019 ◽  
Author(s):  
Luis Gabriel Rivera Calderón ◽  
Priscila Emiko Kobayashi ◽  
Rosemeri Oliveira Vasconcelos ◽  
Carlos Eduardo Fonseca-Alves ◽  
Renee Laufer-Amorim
Keyword(s):  
Extracellular Matrix ◽  
Smooth Muscle ◽  
Blood Vessels ◽  
Basal Membrane ◽  
Elastic Fibers ◽  
Normal Prostate ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Imagej Software ◽  
Area Percentage

Collagen (Coll) is the most common protein in the extracellular matrix, responsible for providing tissue structure and support. In some types of cancer, including prostate cancer (PC) Coll deregulation was described and related to tumor progression and metastasis. This study aimed to investigate Coll-I, III, IV and elastin in canine normal prostate and PC, using Picrosirius red (PSR) and Immunohistochemical (IHC) analysis. Eight normal prostates and 10 PC from formalin-fixed, paraffin-embedded samples were used. Collagen fibers area was analyzed with ImageJ software. The distribution of Coll-I and Coll-III was approximately 80% around prostatic ducts and acini, 15% among smooth muscle and 5% around in blood vessels, in both normal prostate and PC. Immunostaining for Coll-IV was observed in the basal membrane of prostate acini, smooth muscle, blood vessels, and never fibers of normal and PC samples. Elastic fibers were found in the septa dividing the lobules and around the prostatic acini of normal samples. A high amount of elastic fibers was observed around the ducts and the urethra in normal and PC. The distribution and area percentage of staining for collagen are similar in normal and neoplastic canine prostate when analyzed with PSR and IHC.

scholarly journals Histomorphological and Histochemical Studies on Proventriculus in Guinea Fowl (Numida meleagris)

2020 ◽  
Author(s):  
Devendra Saran ◽  
Balwant Meshram
Keyword(s):  
Connective Tissue ◽  
Blood Vessels ◽  
Succinic Dehydrogenase ◽  
Guinea Fowl ◽  
Glandular Cell ◽  
Elastic Fibers ◽  
Significant Activity ◽  
Food Material ◽  
Weak Activity ◽  
Tunica Muscularis

Background: The proventriculus, component of digestive system of bird secretes hydrochloric acid and pepsinogen which helps the churning of food material that takes place in ventriculus. Pepsinogen produces pepsin and it smashes the peptide bonds of amino acid of food material. Methods: The twelve proventriculus samples of Guinea fowl birds were fixed in 10% neutral buffered formalin and Bouin’s fluid for histological procedure while chilled acetone and chilled ethanol were used as the fixatives for histochemical studies. The fresh unfixed samples were used on cryostat for studies of succinic dehydrogenase enzyme.Result: Histomorphologically the proventriculus of Guinea fowl was observed as the typical tubular component with four tunics from inside to outside as tunica mucosa, tunica sub mucosa, tunica muscularis and tunica serosa. The submucosal layer comprised with proventricular submucosal gland (PVSMG) which was encapsulated by elastic fibers. Tunica muscularis was inner longitudinal and outer circular layer, while the tunica serosa was observed with surrounding adipose tissue as the connective tissue constituent to endow the access of nutritional supply in the form of blood vessels. The luminal surface of proventricular submucosal glands has shown the significant activity of glycogen while intercalated, intralobular and interlobular ducts have exhibited the weak activity towards the same. The glandular cell rod of PVSMG, serosal layer and adjoining connective tissue with blood vessels has exhibited very weak activity of alkaline phosphatase. Mucosal folds and lumen of PVSMG has shown the weak activity of acidic phosphatase but the serosal limiting membrane has shown very weak activity. The succinic dehydrogenase activity was seen in the connective tissue septa of submucosal glandular cellular rods and the intense activity was observed at tunica muscularis.

scholarly journals Ultrastructural immunolocalization of elastic fibers in rat blood vessels using the protein A-gold technique.

1987 ◽  
Vol 35 (1) ◽  
pp. 15-21 ◽  
Author(s):  
C Lethias ◽  
D J Hartmann ◽  
M Masmejean ◽  
M Ravazzola ◽  
I Sabbagh ◽  
...  
Keyword(s):  
Blood Vessels ◽  
Protein A ◽  
High Sensitivity ◽  
Ultrastructural Level ◽  
Rat Aorta ◽  
Elastic Fibers ◽  
Thin Sections ◽  
Rat Blood ◽  
Human Dermis ◽  
Arteries And Veins

Identification of elastic fibers at the ultrastructural level is accomplished by a post-embedding immunohistochemical technique using the protein A-colloidal gold method. Antisera against elastins from human dermis and rat aorta have been characterized by radioimmunoassay and then applied to thin sections of rat blood vessels. Two fixative solutions and two embedding media have been tested. Both antibodies bind to elastic fibers of normal arteries and veins, indicating crossreactions among organs and species. The high sensitivity of this method is demonstrated by its application to the detection of neo-elastogenesis in the intimal thickening of aortic grafts.

An increase of elastic tissue fibers in blood vessel walls of placental stem villi and differences in the thickness of blood vessel walls in third trimester pre-eclampsia pregnancies

Open Medicine ◽  
2010 ◽  
Vol 5 (2) ◽  
pp. 227-234 ◽  
Author(s):  
Özlem Baran ◽  
Mehmet Tuncer ◽  
Yusuf Nergiz ◽  
Murat Akkuş ◽  
Mahmut Erdemoğlu ◽  
...  
Keyword(s):  
Blood Vessel ◽  
Blood Vessels ◽  
Elastic Fiber ◽  
Elastic Fibers ◽  
Elastic Tissue ◽  
Fiber System ◽  
Tissue Samples ◽  
Terminal Villi ◽  
Vessel Walls ◽  
Stem Villi

AbstractThis study has goals of examining whether pre-eclampsia may lead to an increase of elastic tissue fibers in blood vessel walls of placental stem villi or whether there are differences in the thickness of blood vessel walls within these villi when compared to normotensive pregnant women. Non-infarcted placental tissue samples from 28 participants with uncomplicated pregnancies and 26 patients with pre-eclampsia were obtained. After routine histological procedures, the sections were processed either for conventional Verhoeff staining for the demonstration of elastic fiber system. Paraffine sections from placenta biopsies prepared for light microscopic examination were gathered. In uncomplicated pregnancies, terminal villi blood vessels were observed with no stained elastic tissue fibers in most areas. In the pre-eclampsia pregnancy of human placenta, the elastic fibers significiantly increased in terminal villi blood vessel walls which were dark in color, using Verhoeff’s tissue stain, when comparing with the uncomplicated pregnancy group. Our results indicate that an increase of elastic tissue fibers in blood vessels of placental stem villus and terminal villi, and also an increase of wall thickness during pre-eclampsia.

scholarly journals Photooxidation crosslinking to recover residual stress in decellularized blood vessel

2021 ◽  
Vol 8 (2) ◽  
Author(s):  
Jintao Wang ◽  
Lingwen Kong ◽  
Alidha Gafur ◽  
Xiaobo Peng ◽  
Natalia Kristi ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Residual Stress ◽  
Blood Vessel ◽  
Blood Vessels ◽  
Vascular Grafts ◽  
Small Diameter ◽  
Opening Angle ◽  
Elastic Fibers ◽  
Transmission Electron ◽  
Small Diameter Vascular Grafts

Abstract Decellularization method based on trypsin-digestion is widely used to construct small diameter vascular grafts. However, this method will reduce the opening angle of the blood vessel and result in the reduction of residual stress. Residual stress reduced has an adverse effect on the compliance and permeability of small diameter vascular grafts. To improve the situation, acellular blood vessels were treated with glutaraldehyde and photooxidation crosslinking respectively, and the changes of opening angle, circumferential residual strain of native blood vessels, decellularized arteries and crosslinked blood vessels were measured by means of histological examination, scanning electron microscopy (SEM) and transmission electron microscopy (TEM) in this study. The opening angle of decellularized arteries significantly restored after photooxidation crosslinking (P = 0.0216), while that of glutaraldehyde crosslinking blood vessels reduced. The elastic fibers inside the blood vessels became densely rearranged after photooxidation crosslinking. The results of finite element simulation showed that the residual stress increased with the increase of opening angle. In this study, we found at the first time that photooxidation crosslinking method could significantly increase the residual stress of decellularized vessels, which provides biomechanical support for the development of new biomaterials of vascular grafts.

scholarly journals STUDIES ON THE BLOOD VESSELS IN THE MEMBRANES OF CHICK EMBRYOS

1930 ◽  
Vol 52 (1) ◽  
pp. 81-87 ◽  
Author(s):  
A. E. Cohn ◽  
Fritz Lange
Keyword(s):  
Blood Vessels ◽  
Cross Section ◽  
Single Cells ◽  
Physiological Property ◽  
Elastic Fibers ◽  
Elastic Tissue ◽  
Chick Embryos ◽  
Small Arteries ◽  
Stage Of Development ◽  
Cell Layers

On the basis of the anatomical studies presented the following inferences or conclusions are drawn. 1. In the course of development there appear in the vascular membranes of chick embryos arterial vessels of all calibers, namely, capillaries, small arteries with 2 or 3 cell layers, and large arteries formed of endothelium, longitudinal and circular layers of muscle and adventitia. 2. In none of the stages are elastic fibers developed. Only in the most central portion of the umbilical artery, in that portion namely which is to be regarded as belonging to the embryo, are elastic fibers discoverable. 3. The structure of capillaries is histologically the same at all stages. The small arteries of embryos 10 days old resemble histologically those of 18. At no stage of development are appearances of degeneration nor of fat to be found in arteries. When the physiological results of our investigations are compared with the anatomical ones the following comments may be made. In respect to Paragraph 1 of the anatomical results we may remark that when we study the different forms of the wall of arterial vessels the most delicate vessels consisting of single cells exhibit the greatest irritability. Those which are built of 3 to 4 muscle layers are less irritable. Stouter vessels appearing for the first time at 10 days of incubation require stronger stimuli to bring about the same reaction. In respect to Paragraph 2 of the anatomical results we may make this comment. The absence of elastic fibers in all arteries of the embryonic membranes throughout the period of their development is important in defining a physiological property of the larger vessels. The medium and larger vessels, beginning with the 4th day of incubation, contract differently from normal adult human arteries. In the contracted state they appear in cross section not as small replicas of larger circular structures, but take on a new form. During the course of contraction they become flat and appear band-like as would a garden hose when it is compressed by a weight. In examining a vessel so contracted one sees on rotating the vessel either a broad side or a narrow one. It is for this reason that such arteries appear alternately narrow as a line or broad as a band. It is not until the narrow artery is elevated with a hook that its uniform band-like nature becomes evident. The absence of elastic tissue, the presence of which in all probability is mainly responsible for the usual shape of arteries on cross section, permits one to see how the phenomenon which has been described may come about. Concerning Paragraph 3 of the anatomical conclusions we have this to say. According to the histological investigation a stage of degeneration is wanting in the blood vessels of the embryonic membrane in a sense in which one is accustomed to see such changes in other blood vessel systems during the course of life. On the day of hatching the constituent cells and fibers of the arteries of all calibers are anatomically the same as in their early development. These vessels do not die as the result of aging: The nutrient fluid ceases to flow because of contraction of the umbilical vessels. The blood vessels die in complete possession of their physiological irritability and anatomical integrity. The unaltered irritability of blood vessels of the same caliber at all ages is consonant with their unaltered anatomical structure.

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