scholarly journals Ultrastructural immunolocalization of elastic fibers in rat blood vessels using the protein A-gold technique.

1987 ◽  
Vol 35 (1) ◽  
pp. 15-21 ◽  
Author(s):  
C Lethias ◽  
D J Hartmann ◽  
M Masmejean ◽  
M Ravazzola ◽  
I Sabbagh ◽  
...  
Keyword(s):  
Blood Vessels ◽  
Protein A ◽  
High Sensitivity ◽  
Ultrastructural Level ◽  
Rat Aorta ◽  
Elastic Fibers ◽  
Thin Sections ◽  
Rat Blood ◽  
Human Dermis ◽  
Arteries And Veins

Identification of elastic fibers at the ultrastructural level is accomplished by a post-embedding immunohistochemical technique using the protein A-colloidal gold method. Antisera against elastins from human dermis and rat aorta have been characterized by radioimmunoassay and then applied to thin sections of rat blood vessels. Two fixative solutions and two embedding media have been tested. Both antibodies bind to elastic fibers of normal arteries and veins, indicating crossreactions among organs and species. The high sensitivity of this method is demonstrated by its application to the detection of neo-elastogenesis in the intimal thickening of aortic grafts.

scholarly journals Effect of particle size on labeling density for catalase in protein A-gold immunocytochemistry.

1988 ◽  
Vol 36 (1) ◽  
pp. 107-109 ◽  
Author(s):  
S Yokota
Keyword(s):  
Particle Size ◽  
Quantitative Analysis ◽  
Rat Liver ◽  
Protein A ◽  
High Sensitivity ◽  
Thin Sections ◽  
Gold Particles ◽  
Effect Of Particle Size ◽  
Lowicryl K4m ◽  
Liver Peroxisomes

Effect of particle size on labeling intensity in protein A-gold immunocytochemistry was studied. Catalase labeling of rat liver peroxisomes was used as a labeling model. Ultra-thin sections of Lowicryl K4M-embedded rat liver were stained for catalase with protein A-gold (pAg) probes. Five different sizes of colloidal gold probes, from 5 nm to 38 nm in diameter, were prepared. Labeling intensity decreased as the particle size of the pAg probes increased. The highest labeling was obtained by the 5-nm pAg probe and the lowest by the 38-nm pAg probe. Quantitative analysis also showed that labeling density was inversely proportional to the size of gold particles. The results suggest that the pAg probe with small gold particles has high sensitivity.

scholarly journals An improved immunocytochemical method for subcellular localization of serotonin in rat enterochromaffin cells.

1987 ◽  
Vol 35 (3) ◽  
pp. 319-326 ◽  
Author(s):  
O Nilsson ◽  
A Dahlström ◽  
M Geffard ◽  
H Ahlman ◽  
L E Ericson
Keyword(s):  
Osmium Tetroxide ◽  
Secretory Granules ◽  
Protein A ◽  
Ultrastructural Level ◽  
Proximal Colon ◽  
Intracellular Distribution ◽  
Thin Sections ◽  
Ec Cells ◽  
Immunocytochemical Method ◽  
A Minor

Serotonin-like immunoreactivity (5-HT-LI) has been localized at the ultrastructural level in enterochromaffin (EC) cells of rat gastrointestinal tract. Ultra-thin sections of tissues embedded in epoxy resin were incubated with 5-HT antisera and antibody binding sites were visualized with protein A-gold. Three different antisera were compared and were shown to require different fixation regimens for optimal preservation of 5-HT-LI. For one antiserum, tissues fixed in glutaraldehyde and osmium tetroxide could be used to demonstrate 5-HT-LI in EC cells. Immunocytochemical localization of 5-HT can thus be performed with good ultrastructural preservation of tissues. Quantitative evaluation of the intracellular distribution of 5-HT-LI was performed on EC cells from antrum, duodenum, and proximal colon, fixed in glutaraldehyde only. In all three locations, the majority of the gold particles (90%) in EC cells were localized over the dense core of the secretory granules, while a minor fraction (10%) were localized in parts of the cytoplasm devoid of granules. In EC cells fixed in glutaraldehyde and post-fixed in osmium tetroxide, 5-HT-LI was reduced by about 85%, although intracellular distribution was essentially the same as in cells fixed in glutaraldehyde alone. The results indicate that 5-HT in EC cells is stored mainly in secretory granules, with a small fraction of 5-HT being localized outside the granules.

scholarly journals Palladium chloride as a stain for elastin at the ultrastructural level.

1978 ◽  
Vol 26 (8) ◽  
pp. 635-644 ◽  
Author(s):  
S M Morris ◽  
P J Stone ◽  
W A Rosenkrans ◽  
J D Calore ◽  
J T Albright ◽  
...  
Keyword(s):  
Room Temperature ◽  
Ultrastructural Level ◽  
Elastic Fibers ◽  
Palladium Chloride ◽  
Lead Citrate ◽  
Glutaraldehyde Fixation ◽  
Thin Sections ◽  
Mammalian Tissues ◽  
Chemical Studies ◽  
Sodium Borohydride Reduction

Palladium chloride in aqueous solution stains elastic fibers in thin sections of Epon-embedded tissues. When palladium chloride is used with a lead citrate counterstain, high contrast sections with gray to black elastic fibers are obtained. The stain was tested on newborn and adult mammalian tissues and on adult tissues from lower animals. Sections were mounted on stainless steel grids, stained with 1% palladium chloride solution for 5 to 15 min, rinsed thoroughly, and counterstained with lead citrate for 7 min. Palladium chloride staining solution is stable for several months at room temperature and if the stain is filtered immediately before use, contamination of sections is not a problem. Chemical studies indicate that palladium binds directly to purified bovine ligamentum nuchae elastin and that this binding is not affected by glutaraldehyde fixation or by sodium borohydride reduction of elastin. Osmium post-fixation of glutaraldehyde-fixed elastin did significantly lower the amount of palladium bound. Palladium was shown to be chemically bound to sites on the elastin and not weakly associated. The nature of these sites is discussed.

scholarly journals Морфологічні особливості кровоносних судин тимуса новонароджених телят

2017 ◽  
Vol 19 (82) ◽  
pp. 12-15
Author(s):  
Zh. Stegney
Keyword(s):  
Basement Membrane ◽  
Blood Vessels ◽  
Muscle Cells ◽  
Elastic Fibers ◽  
Involuntary Muscle ◽  
Calf Thymus ◽  
The Media ◽  
Thoracic Part ◽  
Newborn Calves ◽  
Arteries And Veins

The blood vessels of thymus of newborn calves were studied by using a complex of histological methods and methods of injection of blood vessels. Calf thymus gland is an unpaired organ consisting of a pair of cervical, unpaired cervical and thoracic lobes. Radiating insertions (septa) extrude from the capsule, dividing organ on lobules. Stroma (6.53 ± 2.33%) consists of loose connective tissue. The base of the lobules is formed by a lymphoid tissue (epithelial with cells of the lymphoid series). The area of the parenchyma of the diurnal calves’ thymus is 80.57 ± 3.46%. The thymus lobes consist of cortex (57.97 ± 3.38%) and medulla (22.60 ± 2.71%). There are concentric, nest-like bodies called Hassall's corpuscles in the medulla. The blood vessels of the thymus are interlobular and intralobular. Interlobular arteries are a continuation of extraorganic arteries. The diameter, caliber and thickness of the wall of arteries and veins, as well as microcirculatory vessels are different, which is due to functional activity. Some blood vessels branch out in the interlobular stroma, while others penetrate into the lobules, where they branch into microcirculatory vessels. In the cortex the blood vessels are radially branched, and in the medulla they form polygonal plexuses. Blood vessels of the thymus are represented by arteries, veins and microcirculatory vessels. The parameters of the wall of intralobular vessels are less than interlobular. The total area of the blood vessels of the thoracic part of the thymus is 12.89 ± 0.97%, interlobular – 4.28 ± 0.61% and intralobular – 8.61 ± 0.54%. The area of interlobular arteries is 11.71 ± 0.41%, which is slightly smaller than the area of interlobular veins (19.09 ± 0.86%).Microcirculatory vessels occupy the smallest area – 2.33 ± 0.05%. The area of intralobular arteries (9.60± 0.83%) is less, and the veins (22.08 ± 0.45%) are larger than interlobular. Microcirculatory vessels occupy the largest area in the lobe – 35.14 ± 0.88%. The wall of arteries and veins consists of intima, media and adventitia. Microcirculatory vessels are represented by arterioles, precapillaries, capillaries, postcapillaries and venules. The wall of arterioles consists of an intima, which is formed by endotheliocytes on the basement membrane, a media, which is made up principally of smooth (involuntary) muscle cells and elastic fibers arranged in roughly spiral layers, and an adventitia. The structure of the precapillary wall is similar to such in arterioles, but only single smooth muscle cells are in the media. The wall of capillaries and venous section of microcirculatory vessels are formed by endotheliocytes and the basement membrane. 

Ultrastructure and Staining of Developing Elastic Tissue

1971 ◽  
Vol 29 ◽  
pp. 244-245
Author(s):  
E. N. Albert
Keyword(s):  
Fine Structure ◽  
Phosphotungstic Acid ◽  
Staining Method ◽  
Rat Aorta ◽  
Uranyl Acetate ◽  
The Other ◽  
Elastic Fibers ◽  
Elastic Tissue ◽  
Lead Citrate ◽  
New Born

Silver tetraphenylporphine sulfonate (Ag-TPPS) was synthesized in this laboratory and used as an electron dense stain for elastic tissue (Fig 1). The procedures for the synthesis of tetraphenylporphine sulfonate and the staining method for mature elastic tissue have been described previously.The fine structure of developing elastic tissue was observed in fetal and new born rat aorta using tetraphenylporphine sulfonate, phosphotungstic acid, uranyl acetate and lead citrate. The newly forming elastica consisted of two morphologically distinct components. These were a central amorphous and a peripheral fibrous. The ratio of the central amorphous and the peripheral fibrillar portion changed in favor of the former with increasing age.It was also observed that the staining properties of the two components were entirely different. The peripheral fibrous component stained with uranyl acetate and/or lead citrate while the central amorphous portion demonstrated no affinity for these stains. On the other hand, the central amorphous portion of developing elastic fibers stained vigorously with silver tetraphenylporphine sulfonate, while the fibrillar part did not (compare figs 2, 3, 4). Based upon the above observations it is proposed that developing elastica consists of two components that are morphologically and chemically different.

Permeability of Endometrial Blood Vessels to Exogenous Horse Radish Peroxidase in Mice. Electron Microscope Study

1973 ◽  
Vol 31 ◽  
pp. 562-563
Author(s):  
M.C. Castillo-Jessen ◽  
A. González-Angulo
Keyword(s):  
Electron Microscopy ◽  
Blood Vessels ◽  
Electron Microscope Study ◽  
Ultrastructural Level ◽  
Low Molecular Weight ◽  
Horse Radish Peroxidase ◽  
Intravascular Injection ◽  
Normal Morphology ◽  
Functional Implications ◽  
Low Molecular Weight Proteins

Information regarding the normal morphology of uterine blood vessels at ultrastructural level in mammals is scarce Electron microscopy studies dealing with endometrial vasculature despite the functional implications due to hormone priming are not available. Light microscopy observations with combined injection of dyes and microradiography along with histochemical studies does not enable us to know the detailed fine structure of the possible various types of blood vessels in this tissue. The present work has been designed to characterize the blood vessels of endometrium of mice as well as the behavior of the endothelium to injection of low molecular weight proteins during the normal estrous cycle in this animal. One hundred and forty female albino mice were sacrificed after intravascular injection of horse radish peroxidase (HRP) at 30 seconds, 5, 15, 30 and 60 minutes.

Where is the prolamine located in rice endosperm?

1990 ◽  
Vol 48 (3) ◽  
pp. 696-697
Author(s):  
Hsin-Kan Wu ◽  
Mei-Chu Chung
Keyword(s):  
Storage Protein ◽  
Sodium Phosphate ◽  
Recent Experiment ◽  
Protein A ◽  
Uranyl Acetate ◽  
Protein Bodies ◽  
Lead Citrate ◽  
Thin Sections ◽  
Rice Endosperm ◽  
Ethanol Series

In one of our earlier papers (Wu et al. 1978), we suggested that glutelin is the major composition of the round storage protein bodies although they also contain relatively more prolamine than the angular one does. Immunochemical studies of Krishnan et al. (1986) later showed the presence of glutelin in the irregularly-shaped (angular) protein bodies while the prolamines were found in the round ones. Our recent experiment using protein A-gold technique found that prolamine is mainly deposited into the angular protein bodies.Small blocks (1 mm3) of 7 DAF (days after flowering) caryopsis of Orvza perennis were fixed with 3% paraformaldehyde and 3% glutaraldehyde in 0.1M sodium phosphate, pH7.4, dehydrated in a graded ethanol series and infiltrated with Spurr’s resin. Thin sections, after gold labeling, were stained with uranyl acetate and lead citrate. Rabbit antibodies were raised against purified prolamine. Protein A-gold sol complex was prepared based on the technique of Horisberger et al. (1977).

Studies on Thrombolysis with Streptokinase

1971 ◽  
Vol 25 (02) ◽  
pp. 354-378 ◽  
Author(s):  
R Gottlob ◽  
L Stockinger ◽  
U Pötting ◽  
G Schattenmann
Keyword(s):  
Electron Microscopy ◽  
Cross Section ◽  
Whole Blood ◽  
Jugular Vein ◽  
Thin Sections ◽  
The Third ◽  
Morphological Findings ◽  
Blood Clots ◽  
Arteries And Veins

SummaryIn vitro whole blood clots of various ages, experimental thrombi produced in the jugular vein of rabbits and human thrombi from arteries and veins were examined in semi-thin sections and by means of electron microscopy.In all types of clots examined a typical course of retraction was found. Retraction starts with a dense excentrical focus which grows into a densification ring. After 24 hours the entire clot becomes almost homogeneously dense; later a secondary swelling sets in.Shortly after coagulation the erythrocytes on the rim of the clot are bi-concave discs. They then assume the shape of crenate spheres, turn into smooth spheres and finally become indented ghosts which have lost the largest part of their contents. In the inner zone, which makes up the bulk of the clot, we observed bi-concave discs prior to retraction. After retraction we see no crenations but irregularly shaped erythrocytes. Once the secondary swelling sets in, the cross-section becomes polygonal and later spherical. After extensive hemolysis we observe the “retiform thrombus” made up of ghosts.Experimental and clinical thrombi present the same morphology but are differentiated from in vitro clots by: earlier hemolysis, immigration of leukocytes, formation of a rim layer consisting of fibrin and thrombocytes, and the symptoms of organization. Such symptoms of organization which definitely will prevent lysis with streptokinase were found relatively late in experimental and clinical thrombi. Capillary buds and capillary loops were never found in clinical thrombi prior to the third month.The morphological findings agree with earlier physical and enzymatic investigations. The observation that phenomena of reorganization occur relatively late and frequently only in the rim areas of large thrombi explains why lytic therapy is possible in some of the chronic obliterations.

Subtypes of α1adrenoceptors in rat blood vessels

1990 ◽  
Vol 190 (1-2) ◽  
pp. 97-104 ◽  
Author(s):  
Han Chide ◽  
Li Jinling ◽  
Kenneth P. Minneman
Keyword(s):  
Blood Vessels ◽  
Rat Blood

Penile Lichen Sclerosus and “Nevus Elasticus”

2021 ◽  
pp. 1-3
Author(s):  
Maiara Ferreira de Souza ◽  
Wendel Souza Kruschewsky ◽  
Nathanael de Freitas Pinheiro Junior ◽  
Daniel Abensur Athanazio
Keyword(s):  
Blood Vessels ◽  
Cutaneous Lesion ◽  
Lichen Sclerosus ◽  
Elastic Fibers ◽  
Deep Dermis

The association between penile lichen sclerosus and striking accumulation of elastic fibers in deep dermis has been described in rare reports, mostly in vulvar lesions. We describe one case of severe balanopreputial adhesions related to lichen sclerosus and this form of elastosis, with no concomitant neoplasia. Aggregates of elastic fibers were seen in deep dermis and in blood vessels. The lesion mirrors nevus elasticus and nevus elasticus vascularis – a well described cutaneous lesion with no known association with lichen sclerosus.

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