scholarly journals Effect and Repair Mechanism of Nano Ag Sponge Dressing Combined with Gelatin-Bletilla Striata Gum/Salvia Miltiorrhiza on Refractory Orthopedic Wounds

2021 ◽  
Vol 2021 ◽  
pp. 1-8
Author(s):  
Chuanjie Jiao ◽  
Ming Deng ◽  
Yonggang Ma ◽  
Geliang Hu
Keyword(s):  
Wound Healing ◽  
Blood Vessels ◽  
Healing Rate ◽  
Salvia Miltiorrhiza ◽  
Collagen Fibers ◽  
Hair Follicles ◽  
Elastic Fibers ◽  
Bletilla Striata ◽  
Ctrl Group ◽  
Tgf Β1

Objective. To explore the effect and mechanism of the sponge dressing on the healing of refractory orthopedic wound, and the gelatin-Bletilla striata gum/Salvia miltiorrhiza nano Ag (GBS-Ag) sponge dressing was prepared. Methods. GBS-Ag sponge dressing was prepared by the freeze-drying method. Twenty male SD rats were randomly divided into the control group (Ctrl group) and GBS-Ag group, with 10 rats in each group, and the rats in the two groups were established a model of back wound infection. The Ctrl group was treated with gauze, while the GBS-Ag group was treated with GBS-Ag sponge dressing. Wound healing rate, blood immune indexes, Ag content in each organ, morphological changes of wound, and expression of transforming growth factor-β1 (TGF-β1) in wound transformation were detected in the two groups of rats. Results. The mechanical properties of GBS-Ag sponge dressing were all in line with the standard, and it had good killing effect on the conventional strain after being incubated for 24 hours. Compared with the Ctrl group, the healing rate and lymphocyte percentage in the GBS-Ag group were significantly increased on day 4 and day 10 ( P < 0.05 ), while the total number of white blood cells and the percentage of neutrophils were significantly decreased ( P < 0.05 ). Compared with Ctrl group, the Ag content in liver, spleen, and kidney of rats in the GBS-Ag group was significantly increased ( P < 0.05 ). The histological results showed that the Ctrl group lacked collagen fibers in the dermis, and the angiogenesis was not rich, accompanied by a large number of inflammatory cell infiltration. The epidermal repair of rats in the GBS-Ag group was complete and partially keratinized, the dermis was rich in collagen fibers, with elastic fibers and new blood vessels, inflammatory cells were rare, and new hair follicles and thick-walled blood vessels were also observed. The expression of TGF-β1 protein in the wounds of rats in the GBS-Ag group was higher than that of the Ctrl group. Conclusion. GBS-Ag sponge dressing had multiple effects of sterilization and promoting wound healing, and its mechanism may be related to promoting the TGF-β1 protein expression.

Histomorphological, Histochemical and Histoenzymic Studies on the Dermis of Skin of Goat (Capra hircus)

2021 ◽  
Author(s):  
U.P. Mainde ◽  
S.B. Banubakode ◽  
N.C. Nandeshwar ◽  
R.Y. Charjan ◽  
S. Sathapathy ◽  
...  
Keyword(s):  
Acid Phosphatase ◽  
Connective Tissue ◽  
Hair Follicle ◽  
Blood Vessels ◽  
Phosphatase Activity ◽  
Acid Phosphatase Activity ◽  
Collagen Fibers ◽  
Hair Follicles ◽  
Body Regions ◽  
Root Sheath

Background: Skin is a versatile organ, which is extremely important for protection, perception, water regulation and wound healing. The skin of goat is considered extremely durable used by the tannery Industry to make rugs and carpet binding.Methods: The present study was conducted on the skin of goats of either sex to study the age wise changes in the histomorphology, histochemistry and histoenzymology of dermis. The skin samples were collected from Nagpur Muncipal Corporation Slaughter house immediately after natural death and subsequently processed and stained for histological, histochemical and histoenzymic studies. The total of 220 samples, 20 each from different body regions namely dorsal neck, lateral neck, ventral neck, dorsal thorax, lateral thorax, ventral thorax, dorsal abdomen, lateral abdomen, ventral abdomen, lateral thigh and medial thigh were collected. The animals were divided into two groups, viz. six to twelve month of age (Gr I) and above one year age (Gr II).Result: It was observed that the dermis was composed of collagen, elastic, reticular fibers, cells of connective tissue, blood vessels, nerves, hair follicles, sweat glands, sebaceous glands and arrector pili muscles in all body regions in Gr I as well as in Gr II. The dermis was divided into thin superficial papillary layer and a thick deep reticular layer. The dermal papillae were the upward projections of superficial papillary layer between epidermal pegs. In reticular layer, fibroblasts were larger, while in papillary layer, fibroblasts were thin long, compressed and spindle shaped. The collagen fibers were more in number as compared to other fibers in dermis and they were densely arranged in papillary layer and parallel with the skin surface below the epidermis. The elastic fibers were present in between the collagen fibers around the blood vessels and hair follicles. The arteriovenous shunt was found surrounded by capsule of connective tissue consisting of smooth muscle cells. The more number of hair follicles at deep part of papillary layer and few follicles were present in reticular layer. The hair follicle composed of outer root sheath, inner root sheath, cortex and medulla. The depth of primary hair follicle increased with the advancement of age. Moderate PAS positive activity was noted in the papillary layer, while it was weak to moderate in reticular layer. The reticular layer of the dermis showed moderate Sudan black B activity for the presence of lipid than the papillary layer. The mild acid phosphatase activity was seen in the papillary and reticular layer of dermis, while moderate acid phosphatase activity was noticed in the blood vessels. A mild alkaline phosphatase activity was present in dermis in Gr I and Gr II. Conclusion: The present study would form a baseline data on the histomorphology, histochemistry and histoenzymology of skin of goat which would be helpful in future research prospective.

scholarly journals Characterization of Collagen Fibers (I, III, IV) and Elastin of Normal and Neoplastic Canine Prostatic Tissues

2019 ◽  
Vol 6 (1) ◽  
pp. 22 ◽  
Author(s):  
Luis Gabriel Rivera Calderón ◽  
Priscila Emiko Kobayashi ◽  
Rosemeri Oliveira Vasconcelos ◽  
Carlos Eduardo Fonseca-Alves ◽  
Renée Laufer-Amorim
Keyword(s):  
Smooth Muscle ◽  
Blood Vessels ◽  
Normal Tissue ◽  
Basal Membrane ◽  
Nerve Fibers ◽  
Collagen Fibers ◽  
Elastic Fibers ◽  
Normal Prostate ◽  
Formalin Fixed Paraffin Embedded ◽  
Imagej Software

This study aimed to investigate collagen (Coll-I, III, IV) and elastin in canine normal prostate and prostate cancer (PC) using Picrosirius red (PSR) and Immunohistochemical (IHC) analysis. Eight normal prostates and 10 PC from formalin-fixed, paraffin-embedded samples were used. Collagen fibers area was analyzed with ImageJ software. The distribution of Coll-I and Coll-III was approximately 80% around prostatic ducts and acini, 15% among smooth muscle, and 5% surrounding blood vessels, in both normal prostate and PC. There was a higher median area of Coll-III in PC when compared to normal prostatic tissue (p = 0.001 for PSR and p = 0.05 for IHC). Immunostaining for Coll-IV was observed in the basal membrane of prostate acini, smooth muscle, blood vessels, and nerve fibers of normal and PC samples. Although there was no difference in Coll-IV area between normal tissue and PC, tumors with Gleason score 10 showed absence of Coll-IV, when compared to scores 6 and 8 (p = 0.0095). Elastic fibers were found in the septa dividing the lobules and around the prostatic acini of normal samples and were statistically higher in PC compared to normal tissue (p = 0.00229). Investigation of ECM components brings new information and should be correlated with prognosis in future studies.

Abnormal Angiogenesis, Neurogenesis and Lymphangiogenesis in the Skin Underlies Delayed Wound Healing in the Sickle Mouse: Acceleration of Healing by Topical Opioids.

Blood ◽  
2005 ◽  
Vol 106 (11) ◽  
pp. 3177-3177 ◽  
Author(s):  
Kalpna Gupta ◽  
Tasneem Poonawala ◽  
Brett K. Levay-Young ◽  
Marna E. Ericson ◽  
Niroop S. Ammbashankar ◽  
...  
Keyword(s):  
Wound Healing ◽  
Blood Vessels ◽  
Laser Scanning ◽  
Wound Repair ◽  
Lymphatic Vessel ◽  
Wound Closure ◽  
Lymphatic Vessels ◽  
Hair Follicles ◽  
Sensory Nerves ◽  
Leg Ulcers

Abstract Chronic non-healing and painful leg ulcers are a serious manifestation of sickle cell disease (SCD). We hypothesized that structurally and functionally aberrant interdependent triad of angiogenesis, neurogenesis and lymphangiogenesis leads to localized ischemic insult, pain and edema, respectively, resulting in non-healing, painful and ulcerative wounds in SCD. We examined the dorsal skin of sickle mice with different severity (mild, NY1DD, medium, hBERK and severe, BERK) for blood vessels, sensory nerves and lymphatic vessels, by immunostaining 100 micron thick cryosections with endothelial specific anti-CD31, PGP9.5 and calcitonin gene related peptide (CGRP) and anti-lymphatic vessel endothelium marker LYVE-1, respectively. Z-series images were acquired using using laser scanning confocal microscopy. We observed a drastic difference in the structure, presentation and localization of all three processes in sickle mice as compared to their controls, which increased with the severity of the model. We found that, [i] epidermal thickening in BERK is 1/2 vs HbABERK (control expressing normal human Hb), [ii] majority of blood vessels localized in the sub-epidermal region in BERK vs dermal tissue in controls, [iii] microvasculature shows disorganization,tortuous, stringy and collapsed vessels in sickle, [iv] open lymphatic vessels arranged in parallel union with hair follicles in controls collapsed to 50 and 90% in diameter in mild and severe sickle models, respectively, while they moved by 90° becoming perpendicular to hair follicles in the severe sickle; [v] Peptidergic-immunoreactive nerve fibres increased with the severity of sickle state in the epidermis; and [vi] CGRP, a mediator in pain transmission is upregulated 2–5 times in sickle vs controls. These data suggest that aberrant, non-functional blood vessels lead to the development of localized hypoxia and ischemia resulting in tissue damage and compression of lymphatics which in turn increase edema and ulceration. Upregulated CGRP on peripheral nerves is suggestive of severe pain associated with sickle ulcers. Opioids interact with endothelium as well as nerves and promote angiogenesis and analgesia, respectively. Therefore, we examined the effect of topically applied opioids on healing of ischemic wounds in hBERK. In PBS treated HbABERK control, wound healing was 62% vs 42% in sickle on day 12 (p&lt; 0.02). Complete wound closure was observed on day 19 in HbABERK, whereas, complete healing did not occur in sickle mice until 28 days. Topical application of morphine resulted in complete closure of wounds by days 14 and 23 in HbABERK and sickle mice, respectively. Similarly, hydromorphone stimulated complete wound closure as compared to 85% closure with PBS by day 23 in sickle (p&lt;0.03). Blood and lymphatic vessel lengths were 20–35% higher in morphine or hydromorphone vs PBS-cream treated sickle wounds on days 13 and 28, respectively (p&lt;0.05 PBS-Cream vs others on days 13 and 28). Thus wound repair is delayed in SCD due to aberrant vasculature and lymphatics and opioids stimulate normal angiogenesis and lymphangiogenesis which promote wound healing. We speculate that topical opioids may accelerate wound healing in painful non-healing leg ulcers in SCD.

scholarly journals EEFECT OF SUBTILIN OINTMENT ON MORPHOLOGICAL INDICATORS OF WOUND HEALING IN RABBITS

2019 ◽  
Vol 54 (4) ◽  
pp. 80-86
Author(s):  
V.V. Gimranov ◽  
◽  
I.T. Giniyatullin ◽  
Keyword(s):  
Wound Healing ◽  
Connective Tissue ◽  
Granulation Tissue ◽  
Regular Structure ◽  
Collagen Fibers ◽  
Hair Follicles ◽  
Thin Wall ◽  
Fibrous Connective Tissue ◽  
Skin Damage ◽  
Active Regeneration

The paper studies the effect of applying probiotics based Subtilin ointment on morphological indicators of the experimental wound healing in rabbits. The study showed that 3 days after traumatic skin damage and applying Subtilin ointment tissue necrosis was found on the tissue specimen. Marked cell infiltration in interfascicular spaces and swollen collagen fibers were found deep in the skin. The wound surface was covered with a thick fibrin and tissue crust. On day 7 active regeneration of the epithelium proliferating under the crust was observed. Native granulation tissue formed under the newly formed epithelium. On day 14 epithelization and granulation processes resulted in forming fibrous connective tissue. The fibrous collagen elements of the new tissue thickened, formed bundles and prevailed over the cellular elements, fibroblasts. On day 21 the epidermis formed layers (stratum basale, stratum spinosum and stratum corneum). The connective tissue had a developed capillary network, tissues under the regenerating area had regular structure but contained regenerating hair follicles. On day 28 epithelization was completed and two types of regenerated connective tissue formed. The first type was fibrous connective tissue made up of thin bundles of collagen fibers where fibroblastic cells and thin-wall capillaries were found. The second type of regenerated connective tissue was similar to regular connective tissue in structure. By that time only appendages of the skin such as hair sheaths sebaceous and sudoriferous glands could not fully regenerate. Thus, application of Subtilin ointment for treating experimental skin wounds in rabbits relieves signs of inflammation, accelerates complete epithelization of the wound on day 28, and forms native granulation tissue under the epithelium that gradually matures to regenerated connective tissue. The structure of the regenerated connective tissue is similar to that of regular connective tissue.

scholarly journals Localization and Regulation of Pregnancy-Associated Plasma Protein A Expression in Healing Human Skin

2003 ◽  
Vol 88 (9) ◽  
pp. 4465-4471 ◽  
Author(s):  
Bing-Kun Chen ◽  
Kristin M. Leiferman ◽  
Mark R. Pittelkow ◽  
Michael T. Overgaard ◽  
Claus Oxvig ◽  
...  
Keyword(s):  
Wound Healing ◽  
Epithelial Cells ◽  
Blood Vessels ◽  
Human Skin ◽  
Plasma Protein ◽  
Granulation Tissue ◽  
Protein A ◽  
Hair Follicles ◽  
Dermal Fibroblasts ◽  
Igf I

Pregnancy-associated plasma protein A (PAPP-A) is an IGF-binding protein-4 (IGFBP-4) metalloproteinase that cleaves inhibitory IGFBP-4 to amplify local IGF-I bioavailability in vitro. Thus it has functional implications in injury/repair responses. In this study we determined PAPP-A expression in healing human skin. Wounds were induced with a scalpel on the forearms of three normal subjects and were allowed to heal by first intention. Biopsies obtained on d 0, 2, 8, and 14 were processed for immunohistochemical detection of PAPP-A, IGF-I, and IGFBP-4. In uninjured skin (d 0), strong staining for PAPP-A was present in the epidermis, sweat and sebaceous gland epithelial cells, hair follicles, and blood vessels; no PAPP-A was detected in dermal fibroblasts or with mature collagen bundles. IGF-I localized strongly to epithelial cells of skin glands was weak to moderate in epidermis and blood vessels, and was absent in dermal cells. Weak focal staining for IGFBP-4 was found within uninjured epidermis. During wound healing, PAPP-A expression was induced in dermal granulation tissue within and adjacent to the injury. PAPP-A was present in dermis on d 2 and was increased in intensity and extent on d 8 and 14. PAPP-A expression also increased in the epidermis. PAPP-A expression in cells of granulation tissue colocalized with α-smooth actin staining of myofibroblasts and new blood vessels as well as with CD68 staining of macrophages and was associated with the compact, newly synthesized collagen of the healing wound. IGF-I staining was enhanced in the epidermis localized to the area of the incision and in granulation tissue associated with lymphoid cells. IGFBP-4 staining of the epidermis remained unchanged during wound healing, but was induced in the fibroblastic cells of granulation tissue over time. These data demonstrate localized and regulated expression of PAPP-A in human skin and suggest that PAPP-A may play an important role in an integrated IGF system in wound healing and tissue remodeling in vivo.

The Safety and Efficacy of Mupirocin Topical Spray for Burn Wound Healing in A Rat Model

2019 ◽  
Vol 10 (01) ◽  
Author(s):  
Sritharadol Rutthapol ◽  
Chunhachaichana Charisopon ◽  
Kumlungmak Sukanjana ◽  
Buatong Wilaiporn ◽  
Dechraksa Janwit ◽  
...  
Keyword(s):  
Wound Healing ◽  
Matrix Metalloproteinase ◽  
Rat Model ◽  
Transforming Growth Factor ◽  
Immunohistochemical Study ◽  
Enzyme Linked Immunosorbent Assay ◽  
Burn Wound ◽  
Safety And Efficacy ◽  
Burn Wound Healing ◽  
Tgf Β1

ABSTRACT This study evaluated the effect of mupirocin topical spray on burn wound healing in a rat model. Fifteen male Sprague Dawley rats were used to create full-thickness burns on the rat dorsum using a cylindrical stainless steel rod. The rats were topically treated with normal saline solution (NSS), mupirocin spray, ointment, and solution. The wound size and morphological evaluation were investigated by photographs and clinical criterions for wound healing. The histology was observed by hematoxylin and eosin (HandE) staining assay. The immunohistochemical study was evaluated by detection of transforming growth factor-beta 1 (TGF-β1), and the ratio of matrix metalloproteinase-9 to the tissue inhibitor of matrix metalloproteinase-1 (MMP-9/TIMP-1) was quantified using the enzyme-linked immunosorbent assay (ELISA) assay. A complete healing was observed at 28 days in all treatments. Mupirocin formulations accelerated the wound healing faster than NSS in size. However, the clinical criteria indicated a desirable skin appearance in the mupirocin spray and ointment treated groups. The histological evaluations showed no differences between the treatments while the immunohistochemical study revealed that all treatments reduced the level of TGF-β1 over time, particularly on day 28 in the mupirocin spray and ointment treated groups. The MMP-9/TIMP-1 ratio was significantly lower in the mupirocin spray and ointment treated groups than in the NSS and mupirocin solution groups. This study shows the safety and efficacy in the use of mupirocin topical spray. The topical mupirocin spray is an alternative suitable for development as a human topical anti-infective and wound protection spray.

Role of topical insulin therapy as an adjunct in the management of pressure ulcer

2020 ◽  
Vol 2 (3) ◽  
pp. 01-03
Author(s):  
Ravi Chittoria
Keyword(s):  
Wound Healing ◽  
Insulin Therapy ◽  
Pressure Ulcer ◽  
Healing Rate ◽  
Pressure Sore ◽  
Pressure Sores ◽  
Established Method ◽  
Wound Bed Preparation ◽  
Wound Healing Rate

Pressure ulcer or pressure sore is one of the complications seen in bedridden patients. Management of these ulcers is often challenging. But there is no well-established method that accelerates the wound healing rate. Various adjunctive methods are used for wound bed preparation before definitive reconstruction plan is made. Here we describe our experience in the role of insulin therapy as an adjunct in the management of pressure sores.

scholarly journals The Effects of Silver-Releasing Foam Dressings on Diabetic Foot Ulcer Healing

2021 ◽  
Vol 10 (7) ◽  
pp. 1495
Author(s):  
Yu-Chi Wang ◽  
Hsiao-Chen Lee ◽  
Chien-Lin Chen ◽  
Ming-Chun Kuo ◽  
Savitha Ramachandran ◽  
...  
Keyword(s):  
Wound Healing ◽  
Diabetic Foot ◽  
Healing Rate ◽  
Early Period ◽  
Foot Ulcer ◽  
Diabetic Foot Ulcer ◽  
Wound Management ◽  
Control Group ◽  
Diabetic Patients ◽  
High Morbidity

Diabetic foot ulcers (DFUs) are a serious complication in diabetic patients and lead to high morbidity and mortality. Numerous dressings have been developed to facilitate wound healing of DFUs. This study investigated the wound healing efficacy of silver-releasing foam dressings versus silver-containing cream in managing outpatients with DFUs. Sixty patients with Wagner Grade 1 to 2 DFUs were recruited. The treatment group received silver-releasing foam dressing (Biatain® Ag Non-Adhesive Foam dressing; Coloplast, Humlebaek, Denmark). The control group received 1% silver sulfadiazine (SSD) cream. The ulcer area in the silver foam group was significantly reduced compared with that in the SSD group after four weeks of treatment (silver foam group: 76.43 ± 7.41%, SSD group: 27.00 ± 4.95%, p < 0.001). The weekly wound healing rate in the silver foam group was superior to the SSD group during the first three weeks of treatment (p < 0.05). The silver-releasing foam dressing is more effective than SSD in promoting wound healing of DFUs. The effect is more pronounced in the initial three weeks of the treatment. Thus, silver-releasing foam could be an effective wound dressing for DFUs, mainly in the early period of wound management.

scholarly journals Sustainable Development of Chitosan/Calotropis procera-Based Hydrogels to Stimulate Formation of Granulation Tissue and Angiogenesis in Wound Healing Applications

Molecules ◽  
2021 ◽  
Vol 26 (11) ◽  
pp. 3284
Author(s):  
Muhammad Zahid ◽  
Maria Lodhi ◽  
Zulfiqar Ahmad Rehan ◽  
Hamna Tayyab ◽  
Talha Javed ◽  
...  
Keyword(s):  
Wound Healing ◽  
Blood Vessels ◽  
Granulation Tissue ◽  
Chorioallantoic Membrane ◽  
Calotropis Procera ◽  
Connective Tissues ◽  
Blood Capillaries ◽  
Chick Chorioallantoic Membrane ◽  
Thaw Cycle ◽  
Freeze Thaw Cycle

The formation of new scaffolds to enhance healing magnitude is necessarily required in biomedical applications. Granulation tissue formation is a crucial stage of wound healing in which granulation tissue grows on the surface of a wound by the formation of connective tissue and blood vessels. In the present study, porous hydrogels were synthesized using chitosan incorporating latex of the Calotropis procera plant by using a freeze–thaw cycle to stimulate the formation of granulation tissue and angiogenesis in wound healing applications. Structural analysis through Fourier transform infrared (FTIR) spectroscopy confirmed the interaction between chitosan and Calotropis procera. Latex extract containing hydrogel showed slightly higher absorption than the control during water absorption analysis. Thermogravimetric analysis showed high thermal stability of the 60:40 combination of chitosan (CS) and Calotropis procera as compared to all other treatments and controls. A fabricated scaffold application on a chick chorioallantoic membrane (CAM) showed that all hydrogels containing latex extract resulted in a significant formation of blood vessels and regeneration of cells. Overall, the formation of connective tissues and blood capillaries and healing magnitude decreased in ascending order of concentration of extract.

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