scholarly journals Downregulation of Arntl mRNA Expression in Women with Hypertension: A Case-Control Study

2021 ◽  
pp. 1-8
Author(s):  
Zhengmei Fang ◽  
Lijun Zhu ◽  
Yuelong Jin ◽  
Yan Chen ◽  
Weiwei Chang ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Negative Correlation ◽  
Mononuclear Cells ◽  
Case Control Study ◽  
Clock Genes ◽  
Quantitative Polymerase Chain Reaction ◽  
Significant Negative Correlation ◽  
Case Control ◽  
Peripheral Blood Mononuclear ◽  
Control Study

<b><i>Background:</i></b> Previous studies have reported that disturbance of endogenous circadian rhythms enhances the chance of hypertension and suggested that circadian clock genes could have a crucial function in the onset of the disease. This case-control study was aimed to investigate the association of the mRNA expression of aryl hydrocarbon receptor nuclear translocator like (<i>Arntl</i>), clock circadian regulator (<i>Clock</i>), and period circadian regulators 1 and 2 (<i>Per1</i> and <i>Per2</i>) with hypertension and blood pressure levels. <b><i>Methods:</i></b> A total of 172 subjects were recruited in this study, including 86 hypertension and 86 nonhypertension controls. The mRNA expression levels in peripheral blood mononuclear cells were determined by real-time quantitative polymerase chain reaction. The differences in <i>Arntl</i>, <i>Clock</i>, <i>Per1</i>, and <i>Per2</i> mRNA expression were compared between the 2 groups, and the relationship between mRNA expression and cardiometabolic risk profiles was also assessed. <b><i>Results:</i></b> We found that the mRNA expression of <i>Arntl</i> was downregulated in the hypertension cases compared with controls in women (1.10 [0.66, 1.71] vs. 1.30 [0.99, 2.06], <i>p</i> = 0.031). There was a significant negative correlation between the <i>Arntl</i> mRNA expression and SBP (<i>r</i> = −0.301, <i>p</i> = 0.004) and DBP (<i>r</i> = −0.222, <i>p</i> = 0.034) in women. In men, a negative correlation between the <i>Per1</i> mRNA expression and SBP (<i>r</i> = −0.247, <i>p</i> = 0.026) was found. <b><i>Conclusions:</i></b> The <i>Arntl</i> mRNA expression may play an important role in progression of hypertension in women.

scholarly journals Job stress and resilience in Iranian nurses during the COVID-19 pandemic: A case-control study

Work ◽  
2021 ◽  
pp. 1-10
Author(s):  
Gholam-Abbas Shirali ◽  
Arman Amiri ◽  
Khalil Taherzadeh Chanani ◽  
Maryam Silavi ◽  
Sanaz Mohipoor ◽  
...  
Keyword(s):  
Job Stress ◽  
Negative Correlation ◽  
Case Control Study ◽  
Case Control ◽  
Target Group ◽  
Control Group ◽  
Wide Range ◽  
The Face ◽  
And Control ◽  
Control Study

BACKGROUND: The COVID-19 pandemic has created a wide range of sociocultural pressures on nurses. Resilience is defined as one’s ability to adapt to an unpredictable situation and it can be a factor in accepting an undesirable psychosocial situation. OBJECTIVE: The aim of the present study was to examine resilience in nurses in the face of job stress during the COVID-19 pandemic. METHODS: The study was carried out as a case-control study with participation of 400 nurses as the target group (nurses exposed to COVID-19 patients) and the control group (nurses not exposed to COVID-19 patients). To examine resilience and job stress, Conor and Davidson’s questionnaire and OSIPOW questionnaire were used respectively. RESULTS: The mean scores of job stress and resilience were significantly different between the target and control groups (p <  0.05). So that resilience in the target group was less than that in the control group. In addition, job stress in the target group was higher than that of the control group (p <  0.05). There was a significant and negative correlation between resilience and job stress and the correlation was stronger in the target group (p <  0.05). CONCLUSIONS: Given the high job stress score in the participants and its negative correlation with resilience, there is need to provide the health personnel with efficient preventive and treatment approaches, improve and educate the principles of resilience, improve mental health services system, and introduce programs to control some of demographical factors in job stress such as physical activity, and employment status of nurses.

scholarly journals Associations of Genetic Variation in Glyceraldehyde 3-Phosphate Dehydrogenase Gene with Noise-Induced Hearing Loss in a Chinese Population: A Case-Control Study

2020 ◽  
Vol 17 (8) ◽  
pp. 2899 ◽  
Author(s):  
Liu Wan ◽  
Boshen Wang ◽  
Juan Zhang ◽  
Baoli Zhu ◽  
Yuepu Pu
Keyword(s):  
Hearing Loss ◽  
Genetic Variation ◽  
Chinese Population ◽  
Case Control Study ◽  
Quantitative Polymerase Chain Reaction ◽  
Case Control ◽  
Control Group ◽  
Case Group ◽  
Noise Induced Hearing Loss ◽  
Control Study

Objective: The purpose of this paper was to clarify the association between genetic variation in the glyceraldehyde 3-phosphate dehydrogenase (GAPDH) gene and the risk of noise-induced hearing loss (NIHL). Methods: A case-control study (633 cases and 625 controls) was conducted in this study. Logistic regression was used to analyze the relationships between environmental and individual factors and NIHL. Gene expression levels were compared among each GAPDH rs6489721 genotype and between the case and control groups based on real-time fluorescence quantitative Polymerase Chain Reaction (PCR). Results: The T allele of GADPH rs6489721 was significantly associated with NIHL (odds ratio (OR) = 1.262, 95% confidence interval (CI) (1.066, 1.493), p = 0.006) and showed strong associations in the codominant and dominant models (TT vs. CC: OR = 1.586, 95% CI (1.131, 2.225), p = 0.008; TT vs. TC/CC: OR = 1.391, 95% CI (1.073, 1.804), p = 0.013). The expression level of the TT genotype was significantly higher than that of the CC genotype (p = 0.012), and the expression of the case group was also higher than that of the control group (p = 0.013). Conclusions: The homozygous risk allele (TT) of rs6489721 was associated with an enhanced GAPDH expression, resulting in the development of NIHL in a Chinese population.

Recurrent miscarriage is associated with increased ghrelin mRNA expression in the endometrium- a case-control study

2018 ◽  
Vol 18 (1) ◽  
pp. 12-17 ◽  
Author(s):  
Aneta Zwierzchowska ◽  
Anna Iwan ◽  
Anna Hyc ◽  
Barbara Suchońska ◽  
Jacek Malejczyk ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Case Control Study ◽  
Recurrent Miscarriage ◽  
Case Control ◽  
Control Study

scholarly journals Combined Effect of Telomere Length and Mitochondrial DNA Copy Number As a Potential Biomarker Indicating PE Risk: a Case-Control Study in a Chinese Population

2022 ◽  
Author(s):  
Ruyi Zhang ◽  
Jiangbo Du ◽  
Zhendong Xiao ◽  
Yuan Jiang ◽  
Qiao Weng ◽  
...  
Keyword(s):  
Cord Blood ◽  
Telomere Length ◽  
Copy Number ◽  
Case Control Study ◽  
Quantitative Polymerase Chain Reaction ◽  
Maternal Blood ◽  
Case Control ◽  
Combined Effect ◽  
Potential Biomarker ◽  
Control Study

Abstract Purpose To explore changes of Telomere length (TL) and mitochondrial copy number (mtDNA-CN) in preeclampsia (PE) and to evaluatethe combined effect of maternal TL and mtDNA-CN on PE risk.Methods A case-control study of 471 subjects (130 PE cases and 341 age frequency matched controls) was conducted in Nanjing Drum Tower Hospital, Jiangsu Province of China. Relative telomere length (RTL) and mtDNA-CN were measured using quantitative polymerase chain reaction (qPCR) and PE risk was calculated between groups by logistic regression analyses.Results PE patients displayed longer RTL (0.48 versus 0.30) and higher mtDNA-CN (3.02 versus 2.00) in maternal bloodas well as longer cord blood RTL(0.61 versus 0.35) but lower mtDNA-CN (1.69 versus 5.49) in cord blood (all p<0.001). Exercise during pregnancy exerted an obvious effect of prolonging maternal telomere length. Multiparous, women with folic acid intake during early pregnancy and those delivered vaginally showed longer telomere length while those factors imposed no or opposite effect on RTL in PE cases. Furthermore, RTL and mtDNA-CN were positively correlated in controls (in maternal blood r=0.18, p<0.01; in cord blood r=0.19, p<0.001), but this correlation was disrupted in PE cases, no matter in maternal blood or in cord blood. Longer maternal RTL and higher mtDNA-CN were associated with higher risk of PE, and the ROC curve of RTL and mtDNA-CN in predicting PE risk presented an AUC of 0.755(95%CI: 0.698-0.812).Conclusions Interaction of TL and mtDNA-CN may play an important role in pathogenesis of PE and it could be a potential biomarker indicating PE risk.

scholarly journals Hsa-miR-27a-3p overexpression in men with nonobstructive azoospermia: A case-control study

2020 ◽  
Author(s):  
Hamid Norioun ◽  
Majid Motovali-bashi ◽  
Seyed Morteza Javadirad
Keyword(s):  
Male Infertility ◽  
Case Control Study ◽  
Quantitative Polymerase Chain Reaction ◽  
Case Control ◽  
Micro Rna ◽  
Expression Level ◽  
Obstructive Azoospermia ◽  
Aberrant Expression ◽  
Micro Rnas ◽  
Control Study

Background: The role of KDM3A and its downstream genes in male fertility has been approved in animal models. Additionally, the expression shrinkage of KDM3A is significantly correlated with human azoospermia phenotype. Aberrant expression of micro-RNAs could mislead spermatogenesis and mostly lead to diverse phenotypes of male infertility. Objective: The aim of this study was to evaluate the expression level of hsa-miR-27a- 3p in azoospermic men to reveal its possible association with infertility. Materials and Methods: This case-control study was conducted on 30 azoospermic men, of whom, 19 had non obstructive azoospermia (NOA) and 11 obstructive azoospermia (OA) according to the pathological examinations. Comprehensive bioinformatics investigations were performed securely and hsa-miR-27a-3p was selected afterward. Reverse Transcriptase-quantitative polymerase chain reaction (RTqPCR) method was used and statistical analysis was performed to compare the expression level of hsa-miR-27a-3p in both OA and NOA individuals. Results: In silico analysis suggested hsa-miR-27a-3p, with its potential binding ability to target KDM3A transcripts. The expression analysis of candidate hsa-miR-27a-3p indicated its significant overexpression in NOA men. Conclusion: The hsa-miR-27a-3p was overexpressed in NOA men compared to OA-control individuals. As a consequence, the overexpressed micro-RNA could downregulate directly KDM3A and indirectly TNP1 and PRM1. Therefore, spermatogenesis could be misled and male infertility could be developed. Key words: hsa-miR-27a-3p, Male infertility, KDM3A.

scholarly journals Upregulation of Apoptosis Pathway Genes in Peripheral Blood Mononuclear Cells of HIV-Infected Individuals with Antiretroviral Therapy-Associated Mitochondrial Toxicity

2017 ◽  
Vol 61 (8) ◽  
Author(s):  
Yram Foli ◽  
Musie Ghebremichael ◽  
Min Li ◽  
Elijah Paintsil
Keyword(s):  
Antiretroviral Therapy ◽  
Mononuclear Cells ◽  
Case Control Study ◽  
Mitochondrial Toxicity ◽  
Peripheral Blood Mononuclear ◽  
Apoptosis Pathway ◽  
Causal Pathway ◽  
Study Participants ◽  
Upregulated Genes ◽  
Control Study

ABSTRACT A case-control study of the effect of antiretroviral therapy (ART) on apoptosis pathway genes comprising 16 cases (HIV infected with mitochondrial toxicity) and 16 controls (HIV uninfected) was conducted. A total of 26 of 84 genes of the apoptosis pathway were differentially expressed. Two of the upregulated genes, DFFA and TNFRSF1A, classified 75% of study participants correctly as either a case or control. Thus, apoptosis may be in the causal pathway of ART-associated mitochondrial toxicity. These two genes could be markers for detecting and monitoring ART-induced mitochondrial toxicity.

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