The flavonoids rutin and quercetin and two of their decomposition products, phloroglucinol and protocatechuic acid, were rapidly degraded in soil. Less than 10% of their phenolic nuclei were detected in soil, and from 27 to 57% of their structural carbons remained in soil after 9 weeks of incubation. Phenolic intermediates were not detected in soils amended with rutin or quercetin. There were no differences in the decomposition of any of the substrates in noninfested soil and in soils infested with F. solani f. sp. phaseoli. In pure culture, two virulent isolates of the pathogen could effectively metabolize a glucose–rhamnose mixture and protocatechuic acid, but only one of the isolates could use phloroglucinol and rutin to any great extent. When incorporated into soil at a rate of 0.1% and 0.3%, rutin, phloroglucinol, protocatechuic acid, and a glucose–rhamnose mixture significantly increased propagule numbers of the pathogen over that of the control. From 1 week, to the termination of the experiment after 9 weeks, more than 80% of the propagules of F. solani f. sp. phaseoli existed in the soils as chlamydospores. Propagule numbers were higher in soils amended with 0.3% substrate than in soils amended with 0.1% substrate, and the numbers 1 week after amendment addition were similar to those obtained 9 weeks after amendment addition. In the first and second crops of beans (planted 3 and 6 weeks after amendment incorporation), protocatechuic acid and phloroglucinol, at a concentration of 0.3%, were very effective in reducing root-rot severity. The other amendments, except quercetin, were effective in suppressing disease to various degrees. The effectiveness of the various amendments depended upon amendment concentration and time of planting. Quercetin, added to soil at either concentration, did not increase inoculum density but did increase disease severity.
Hepatitis C (HCV) therapy for HCV mono-infected and HIV-HCV co-infected individuals living in Nepal