scholarly journals Pinostilbene Hydrate Suppresses Human Oral Cancer Cell Metastasis by Downregulation of Matrix Metalloproteinase-2 Through the Mitogen-Activated Protein Kinase Signaling Pathway

2018 ◽  
Vol 50 (3) ◽  
pp. 911-923 ◽  
Author(s):  
Ming-Ju Hsieh ◽  
Mei-Chung Chin ◽  
Chia-Chieh Lin ◽  
Yi-Ting His ◽  
Yu-Sheng Lo ◽  
...  
Keyword(s):  
Oral Cancer ◽  
Cell Lines ◽  
Cancer Cell ◽  
Mitogen Activated Protein Kinase ◽  
Matrix Metalloproteinase 2 ◽  
Migration And Invasion ◽  
Protein Activity ◽  
Oral Cancer Cell ◽  
Cell Metastasis ◽  
Mitogen Activated Protein

Background/Aims: Cancer is the most common cause of death worldwide with approximately one third of people being diagnosed with cancer in their lifetime. Pinostilbene hydrate (PSH) A methylated derivative of resveratrol Has been reported to possess antioxidative Cardioprotective and anticancer properties. However the antimetastatic effect of pinostilbene in oral squamous cell carcinoma (OSCC) remains unknown. Methods: In this study We investigated the effect of PSH on antimetastatic activity and the relevant signaling pathways underlying mechanisms of SCC-9 SAS and HSC-3 oral cancer cell lines by MTT assay Wound healing Transwell assay Zymography and western blot analysis. Results: Our findings indicated that PSH inhibits migration and invasion ability by reducing the protein activity and expression of matrix metalloproteinases-2 (MMP-2) in all three cell lines. Moreover • The phosphorylation of extracellular signal-regulated kinase (ERK) and p38 mitogen-activated protein kinases (p38) had significant inhibitory effects in the presence of PSH in the SCC9 and SAS cell lines. A combination of ERK1/2 and p38 inhibitors with PSH also reduced the migration and activity of MMP-2 in the SCC9 and SAS cell lines. Conclusion: This study demonstrated that PSH suppresses MMP-2 enzymatic activity by downregulating the p38/ERK1/2 pathway and that it might be a promising agent for preventing OSCC cell metastasis.

scholarly journals Caffeic Acid Phenethyl Ester Inhibits Oral Cancer Cell Metastasis by Regulating Matrix Metalloproteinase-2 and the Mitogen-Activated Protein Kinase Pathway

2012 ◽  
Vol 2012 ◽  
pp. 1-10 ◽  
Author(s):  
Chih-Yu Peng ◽  
Hui-Wen Yang ◽  
Yin-Hung Chu ◽  
Yu-Chao Chang ◽  
Ming-Ju Hsieh ◽  
...  
Keyword(s):  
Oral Cancer ◽  
Matrix Metalloproteinase ◽  
Caffeic Acid ◽  
Cancer Cell ◽  
Cancer Metastasis ◽  
Caffeic Acid Phenethyl Ester ◽  
Matrix Metalloproteinase 2 ◽  
Migration And Invasion ◽  
Oral Cancer Cell ◽  
Cell Metastasis

Caffeic acid phenethyl ester (CAPE), an active component extracted from honeybee hives, exhibits anti-inflammatory and anticancer activities. However, the molecular mechanism by which CAPE affects oral cancer cell metastasis has yet to be elucidated. In this study, we investigated the potential mechanisms underlying the effects of CAPE on the invasive ability of SCC-9 oral cancer cells. Results showed that CAPE attenuated SCC-9 cell migration and invasion at noncytotoxic concentrations (0 μM to 40 μM). Western blot and gelatin zymography analysis findings further indicated that CAPE downregulated matrix metalloproteinase-2 (MMP-2) protein expression and inhibited its enzymatic activity. CAPE exerted its inhibitory effects on MMP-2 expression and activity by upregulating tissue inhibitor of metalloproteinase-2 (TIMP-2) and potently decreased migration by reducing focal adhesion kinase (FAK) phosphorylation and the activation of its downstream signaling molecules p38/MAPK and JNK. These data indicate that CAPE could potentially be used as a chemoagent to prevent oral cancer metastasis.

scholarly journals Interleukin-6 and Interleukin-8 Regulate STAT3 Activation Migration/Invasion and EMT in Chrysophanol-Treated Oral Cancer Cell Lines

Life ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 423
Author(s):  
Po-Chih Hsu ◽  
Yi-Hsuan Chen ◽  
Ching-Feng Cheng ◽  
Chan-Yen Kuo ◽  
Huey-Kang Sytwu
Keyword(s):  
Tumor Microenvironment ◽  
Oral Cancer ◽  
Cell Lines ◽  
Cancer Cell ◽  
Cancer Cell Lines ◽  
Migration And Invasion ◽  
Mesenchymal Transition ◽  
Oral Cancer Cell ◽  
Stat3 Phosphorylation ◽  
E Cadherin

The tumor microenvironment plays a critical role in the control of metastasis. The epithelial–mesenchymal transition (EMT) is strongly associated with tumor metastasis, and consists of several protein markers, including E-cadherin and vimentin. We discovered that chrysophanol causes oral cancer cell apoptosis and the inhibition of migration/invasion and EMT. However, the detailed mechanisms of chrysophanol and its role in oral cancer with respect to the tumor microenvironment remain unknown. In the clinic, proinflammatory cytokines, such as IL-6 and IL-8, exhibit a higher expression in patients with oral cancer. However, the effect of chrysophanol on the production of IL-6 and IL-8 is unknown. We evaluated the expression of IL-6 and IL-8 in human SAS and FaDu oral cancer cell lines in the presence or absence of chrysophanol. The migration and invasion abilities were also determined using a Boyden chamber assay. Our results showed that treatment with chrysophanol significantly decreased the expression of IL-6 and IL-8, as well as the invasion ability of oral cancer cells. Moreover, chrysophanol also attenuated the EMT by increasing the expression of E-cadherin and reducing the expression of vimentin. Mechanistically, chrysophanol inhibited IL-6- and IL-8-induced invasion and STAT3 phosphorylation. IL-6 and IL-8 promote EMT and cell invasion, which is potentially related to the STAT3 signaling pathway in oral cancer. These findings provide insight into new aspects of chrysophanol activity and may contribute to the development of new therapeutic strategies for oral cancer.

scholarly journals PRELIMINARY PHYTOCHEMICAL ANALYSIS AND CYTOTOXICITY POTENTIAL OF PINEAPPLE EXTRACT ON ORAL CANCER CELL LINES

2016 ◽  
pp. 140
Author(s):  
Anirudh Menon ◽  
Vishnu Priya V ◽  
Gayathri R
Keyword(s):  
Oral Cancer ◽  
Cell Lines ◽  
Cancer Cell ◽  
Anticancer Drug ◽  
Cancer Cell Lines ◽  
Phytochemical Analysis ◽  
Phytochemical Composition ◽  
Tetrazolium Bromide ◽  
Oral Cancer Cell ◽  
Natural Way

<p>ABSTRACT<br />Objective: This study aims at performing a preliminary phytochemical analysis to evaluate the phytochemical composition of pineapple extract and<br />its cytotoxicity potential on oral cancer cell lines.<br />Methods: Preliminary phytochemical analysis of pineapple extract was done, 3-(4, 5-Dimethyl-2-thiazolyl)-2, 5-diphenyl-2H-tetrazolium bromide<br />assay for evaluating the cytotoxicity potential of the extract on oral cancer cell lines was performed.<br />Results: Phytoconstituents such as flavonoids, coumarins, and phenols were present in the pineapple extract. The extract also exhibited increased<br />cytotoxicity with increased concentration.<br />Conclusion: This study is conducted to see if pineapple extract is effective in treating oral cancer in a natural way instead of harmful treatments.<br />Keywords: Cytotoxicity, Pineapple extract, Anticancer drug.</p><p> </p>

scholarly journals Progesterone receptor membrane component 1 is involved in oral cancer cell metastasis

2020 ◽  
Vol 24 (17) ◽  
pp. 9737-9751
Author(s):  
Hsun‐Yu Huang ◽  
Hsiu‐Chuan Chou ◽  
Ching‐Hsuan Law ◽  
Wan‐Ting Chang ◽  
Tzu‐Ning Wen ◽  
...  
Keyword(s):  
Oral Cancer ◽  
Progesterone Receptor ◽  
Cancer Cell ◽  
Membrane Component ◽  
Receptor Membrane ◽  
Oral Cancer Cell ◽  
Cell Metastasis
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